A-802715

Cat. No.: HY-U00142 Purity: 98.45%: FAQs
Data Sheet SDS COA Handling Instructions

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A802715 is a methylxanthine derivative. A802715 has a TD₅₀ (toxic dose of 50%) of 0.9-1.1 mM.

For research use only. We do not sell to patients.

A-802715 Chemical Structure

CAS No. : 107767-58-8

Size	Price	Stock	Quantity
Solid + Solvent (Highly Recommended)
10 mM * 1 mL in DMSO ready for reconstitution	USD 227	In-stock	Estimated Time of Arrival: December 31
Solution
10 mM * 1 mL in DMSO	USD 227	In-stock	Estimated Time of Arrival: December 31
Solid
5 mg	USD 320	In-stock	Estimated Time of Arrival: December 31
10 mg	USD 510	In-stock	Estimated Time of Arrival: December 31
25 mg	USD 1000	In-stock	Estimated Time of Arrival: December 31
50 mg	USD 1500	In-stock	Estimated Time of Arrival: December 31
100 mg	USD 2250	In-stock	Estimated Time of Arrival: December 31
200 mg		Get quote
500 mg		Get quote

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Description

A802715 is a methylxanthine derivative. A802715 has a TD₅₀ (toxic dose of 50%) of 0.9-1.1 mM.

In Vitro

The toxicity of the methylxanthine derivative A802715 is determined against the two human melanoma lines, Be11 and MeWo, and against the two human squamous cell carcinoma lines, 4197 and 4451, by vital dye staining assay. A802715 has a TD₅₀ of 0.9-1.1 mM and is the most toxic. In p53 wt cells BrdU incorporations show that the irradiation-induced suppression of S-phase entry is strongly enhanced by A802715. A802715 prolongs the G₂/M block or remain ineffective depending on the p53 status of the cell line^[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Molecular Weight

322.40

Formula

C₁₆H₂₆N₄O₃

CAS No.

107767-58-8

Appearance

Solid

Color

White to off-white

SMILES

O=C(N1CCCCC(C)(O)C)N(C)C2=C(N(CCC)C=N2)C1=O

Shipping

Room temperature in continental US; may vary elsewhere.

Storage

Powder	-20°C	3 years
	4°C	2 years
In solvent	-80°C	2 years
	-20°C	1 year

Solvent & Solubility

In Vitro:

DMSO : 190 mg/mL (589.33 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Preparing
Stock Solutions

Concentration Solvent Mass	1 mg	5 mg	10 mg

1 mM	3.1017 mL	15.5087 mL	31.0174 mL
5 mM	0.6203 mL	3.1017 mL	6.2035 mL
10 mM	0.3102 mL	1.5509 mL	3.1017 mL

View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 2 years; -20°C, 1 year. When stored at -80°C, please use it within 2 years. When stored at -20°C, please use it within 1 year.

Molarity Calculator
Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

This equation is commonly abbreviated as: C₁V₁ = C₂V₂

Concentration _(start)

Volume _(start)

Concentration _(final)

Volume _(final)

In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

Protocol 1

Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline
Solubility: ≥ 7.5 mg/mL (23.26 mM); Clear solution

This protocol yields a clear solution of ≥ 7.5 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (75.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.
Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
Protocol 2

Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in Saline)
Solubility: ≥ 7.5 mg/mL (23.26 mM); Clear solution

This protocol yields a clear solution of ≥ 7.5 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (75.0 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.
Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
Protocol 3

Add each solvent one by one: 10% DMSO 90% Corn Oil
Solubility: ≥ 7.5 mg/mL (23.26 mM); Clear solution

This protocol yields a clear solution of ≥ 7.5 mg/mL (saturation unknown). If the continuous dosing period exceeds half a month, please choose this protocol carefully.
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (75.0 mg/mL) to 900 μL Corn oil, and mix evenly.

In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Please enter your animal formula composition:

DMSO +

Tween-80 +

Saline

Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.

The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).

Calculation results:

Working solution concentration: mg/mL

Method for preparing stock solution: mg drug dissolved in μL DMSO (Stock solution concentration: mg/mL).

The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only. If necessary, please contact MedChemExpress (MCE).

Method for preparing in vivo working solution for animal experiments: Take μL DMSO stock solution, add μL . μL , mix evenly, next add μL Tween 80, mix evenly, then add μL Saline.

If the continuous dosing period exceeds half a month, please choose this protocol carefully.

Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.

Purity & Documentation

Purity: 98.45%

Select Batch:

Data Sheet (272 KB) SDS (252 KB)

COA (246 KB) LCMS (269 KB)

Handling Instructions (2659 KB)

References

[1]. Bohm L, et al. Influence of pentoxifylline, A-802710, propentofylline and A-802715 (Hoechst) on the expression of cell cycle blocks and S-phase content after irradiation damage. Biochim Biophys Acta. 2000 Dec 11;1499(1-2):1-10. [Content Brief]

Cell Assay
^[1]

DNA analysis is performed using a FACScan flow cytometer emitting a 488 nm beam. Red fluorescence from PI emission is collected as a linear signal through a 600 nm bandpass filter and recorded as a measure of total DNA content. Processing the red fluorescence into height, area and width (doublet discrimination mode) eliminated cell doublets. Data are collected in list mode and 10000 events are recorded per sample and displayed as a frequency distribution histogram. Estimates of the percentages of cells in the different periods of postirradiation incubation with marker statistics reveal the time at which the G₂/M block is maximally expressed. These times are used for each cell line as a starting point at which the methylxanthine drugs were added. Cell debris, nuclei doublets and triplets were excluded by gating^[1].

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

References

[1]. Bohm L, et al. Influence of pentoxifylline, A-802710, propentofylline and A-802715 (Hoechst) on the expression of cell cycle blocks and S-phase content after irradiation damage. Biochim Biophys Acta. 2000 Dec 11;1499(1-2):1-10. [Content Brief]

Complete Stock Solution Preparation Table

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg

DMSO	1 mM	3.1017 mL	15.5087 mL	31.0174 mL	77.5434 mL
	5 mM	0.6203 mL	3.1017 mL	6.2035 mL	15.5087 mL
	10 mM	0.3102 mL	1.5509 mL	3.1017 mL	7.7543 mL
	15 mM	0.2068 mL	1.0339 mL	2.0678 mL	5.1696 mL
	20 mM	0.1551 mL	0.7754 mL	1.5509 mL	3.8772 mL
	25 mM	0.1241 mL	0.6203 mL	1.2407 mL	3.1017 mL
	30 mM	0.1034 mL	0.5170 mL	1.0339 mL	2.5848 mL
	40 mM	0.0775 mL	0.3877 mL	0.7754 mL	1.9386 mL
	50 mM	0.0620 mL	0.3102 mL	0.6203 mL	1.5509 mL
	60 mM	0.0517 mL	0.2585 mL	0.5170 mL	1.2924 mL
	80 mM	0.0388 mL	0.1939 mL	0.3877 mL	0.9693 mL
	100 mM	0.0310 mL	0.1551 mL	0.3102 mL	0.7754 mL

A-802715 Related Classifications

Help & FAQs

Do most proteins show cross-species activity?
Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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A-802715

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Complete Stock Solution Preparation Table

A-802715 Related Classifications

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