AR7

Name: AR7
Brand: MedChemExpress
SKU: HY-101106
Rating: 5.0 (8 reviews)

Cat. No.: HY-101106 Purity: 99.38%: FAQs
Data Sheet
SDS

COA
Handling Instructions Technical Support

AR7 is an atypical RARA/RARα (retinoic acid receptor, alpha) antagonist. AR7 specifically activates chaperone-mediated-autophagy (CMA) activity without affecting macroautophagy.

For research use only. We do not sell to patients.

AR7 Chemical Structure

CAS No. : 80306-38-3

Size	Stock	Quantity

Free Sample (0.1 - 0.5 mg)	Apply Now
Solid + Solvent (Highly Recommended)
10 mM * 1 mL in DMSO ready for reconstitution	In-stock	Estimated Time of Arrival: December 31
Solution
10 mM * 1 mL in DMSO	In-stock	Estimated Time of Arrival: December 31
Solid
5 mg	In-stock	Estimated Time of Arrival: December 31
10 mg	In-stock	Estimated Time of Arrival: December 31
25 mg	In-stock	Estimated Time of Arrival: December 31
50 mg	In-stock	Estimated Time of Arrival: December 31
100 mg	In-stock	Estimated Time of Arrival: December 31
200 mg	Get quote
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This product is a controlled substance and not for sale in your territory.

Customer Review

Based on 8 publication(s) in Google Scholar

7 Publications Citing Use of MCE AR7

•Biochem Biophys Res Commun. 2020 Jul 23;528(2):276-284. [Abstract]

: AR7 purchased from MedChemExpress. Usage Cited in: Biochem Biophys Res Commun. 2020 Jul 23;528(2):276-284. [Abstract]; After treated with 1 μM AR7 for 3 days, the protein expression of CD11b, CEBPβ is detected by immunobloting and quantified.

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RXRα RXRβ RXRγ

Biological Activity
Purity & Documentation
References
Customer Review

Description

AR7 is an atypical RARA/RARα (retinoic acid receptor, alpha) antagonist. AR7 specifically activates chaperone-mediated-autophagy (CMA) activity without affecting macroautophagy^[1].

Cellular Effect

Cell Line	Type	Value	Description	References
HepG2	IC₅₀	10 μM Compound: 11	Cytotoxicity against human HepG2 cells in hypoxic condition after 48 hrs by MTT assay Cytotoxicity against human HepG2 cells in hypoxic condition after 48 hrs by MTT assay	[PMID: 19515559]

In Vitro

Treatment with RARA antagonist, AR7 (20 μM; for 16 h), increased lysosomal activity in WT and LRRK2^R1441G KI mutant MEFs^[1].
AR7 (10, 20, 30 uM; 12, 24 hours) has no effect on macroautophagy in NIH 3T3 cells^[2].
Chaperone-mediated autophagy (CMA) contributes to cellular quality control and the cellular response to stress through the selective degradation of cytosolic proteins in lysosomes. Decrease in CMA activity occurs in aging and in age-related disorders. Signaling through the retinoic acid receptor alpha (RARα) inhibits CMA. AR7 significantly activates CMA activity in mouse fibroblasts. A marked increase in CMA-activating potency is found when AR7 and GR1 are combined, supporting their cooperative effect. Treatment with the transcriptional repressor Actinomycin D partially reduces the stimulatory effect of AR7 on CMA, consistent with transcriptional changes contributing to the upregulation of CMA^[3].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Molecular Weight

257.71

Formula

C₁₅H₁₂ClNO

CAS No.

80306-38-3

Appearance

Solid

Color

White to off-white

SMILES

CC1=CC=C(C2=NC3=CC=C(Cl)C=C3OC2)C=C1

Shipping

Room temperature in continental US; may vary elsewhere.

Storage

Powder	-20°C	3 years
	4°C	2 years
In solvent	-80°C	6 months
	-20°C	1 month

Solvent & Solubility

In Vitro:

DMSO : 25 mg/mL (97.01 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Preparing
Stock Solutions

Concentration Solvent Mass	1 mg	5 mg	10 mg

1 mM	3.8803 mL	19.4017 mL	38.8033 mL
5 mM	0.7761 mL	3.8803 mL	7.7607 mL
10 mM	0.3880 mL	1.9402 mL	3.8803 mL

View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Molarity Calculator
Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

This equation is commonly abbreviated as: C₁V₁ = C₂V₂

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In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

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Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Calculation results:

Working solution concentration: mg/mL

Purity & Documentation

Purity: 99.38%

Select Batch:

Data Sheet (273 KB) SDS (399 KB)

COA (246 KB) HNMR (135 KB) LCMS (104 KB)

Handling Instructions (2659 KB)

References

[1]. Philip Wing-Lok Ho, et al. Age-dependent accumulation of oligomeric SNCA/α-synuclein from impaired degradation in mutant LRRK2 knockin mouse model of Parkinson disease: role for therapeutic activation of chaperone-mediated autophagy (CMA). Autophagy. 2020 Feb;16(2):347-370. [Content Brief]

[2]. Mathieu Bourdenx, et al. Chaperone-mediated autophagy prevents collapse of the neuronal metastable proteome. Cell. 2021 May 13;184(10):2696-2714.e25. [Content Brief]

[3]. Anguiano J, et al. Chemical modulation of chaperone-mediated autophagy by retinoic acid derivatives. Nat Chem Biol. 2013 Jun;9(6):374-82. [Content Brief]

Complete Stock Solution Preparation Table

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg

DMSO	1 mM	3.8803 mL	19.4017 mL	38.8033 mL	97.0083 mL
	5 mM	0.7761 mL	3.8803 mL	7.7607 mL	19.4017 mL
	10 mM	0.3880 mL	1.9402 mL	3.8803 mL	9.7008 mL
	15 mM	0.2587 mL	1.2934 mL	2.5869 mL	6.4672 mL
	20 mM	0.1940 mL	0.9701 mL	1.9402 mL	4.8504 mL
	25 mM	0.1552 mL	0.7761 mL	1.5521 mL	3.8803 mL
	30 mM	0.1293 mL	0.6467 mL	1.2934 mL	3.2336 mL
	40 mM	0.0970 mL	0.4850 mL	0.9701 mL	2.4252 mL
	50 mM	0.0776 mL	0.3880 mL	0.7761 mL	1.9402 mL
	60 mM	0.0647 mL	0.3234 mL	0.6467 mL	1.6168 mL
	80 mM	0.0485 mL	0.2425 mL	0.4850 mL	1.2126 mL

AR7 Related Classifications

Help & FAQs

Do most proteins show cross-species activity?
Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

AR780306-38-3AR 7AR-7RAR/RXRRetinoic acid receptorsRetinoid X receptorsretinoicacidreceptorCMAmacroautophagyInhibitorinhibitorinhibit

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AR7

Customer Review

AR7 Related Antibodies

7 Publications Citing Use of MCE AR7

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•Related Small Molecules:

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Biological Activity

Purity & Documentation

References

Customer Review

AR7 Related Antibodies

Molarity Calculator

Dilution Calculator

Complete Stock Solution Preparation Table

AR7 Related Classifications

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SAFETY DATA SHEET (SDS)

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