Anemarrhenasaponin I

Name: Anemarrhenasaponin I
Brand: MedChemExpress
SKU: HY-N4213
Rating: 5.0 (4 reviews)

Cat. No.: HY-N4213 Purity: 99.43%: FAQs
Data Sheet SDS COA Handling Instructions

Molarity Calculator

Anemarrhenasaponin I, a traditional Chinese medicine, shows remarkable inhibiting effect on platelet aggregation.

For research use only. We do not sell to patients.

Anemarrhenasaponin I Chemical Structure

CAS No. : 163047-21-0

Size	Price	Stock	Quantity
Solid + Solvent (Highly Recommended)
10 mM * 1 mL in DMSO ready for reconstitution	USD 150	In-stock	Estimated Time of Arrival: December 31
Solution
10 mM * 1 mL in DMSO	USD 150	In-stock	Estimated Time of Arrival: December 31
Solid
5 mg	USD 90	In-stock	Estimated Time of Arrival: December 31
10 mg	USD 144	In-stock	Estimated Time of Arrival: December 31
50 mg		Get quote
100 mg		Get quote

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Customer Review

Based on 4 publication(s) in Google Scholar

2 Publications Citing Use of MCE Anemarrhenasaponin I

Proliferation Assay

: Anemarrhenasaponin I purchased from MedChemExpress. Usage Cited in: Oncologie. 2023 Mar 10.; Anemarrhenasaponin I (An-I; 25, 50, 75 μM) downregulates the expression level of GLI1 in SKOV3 cells in a concentration-dependent manner.

: Anemarrhenasaponin I purchased from MedChemExpress. Usage Cited in: Oncologie. 2023 Mar 10.; Both Anemarrhenasaponin I (An-I; 25 μM; 24 h) and GANT61 (20 μM; 24 h) can suppress the clonogenic ability of SKOV3 cells remarkably.

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Biological Activity
Purity & Documentation
References
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Description

Anemarrhenasaponin I, a traditional Chinese medicine, shows remarkable inhibiting effect on platelet aggregation.

In Vitro

Six steroidal saponins are isolated from Anemarrhena asphodeloides Bunge (Liliaceae), a traditional chinese medicine, and named Anemarrhenasaponin I (An-I), Anemarrhenasaponin Ia (An-Ia), Timosaponin B-I (TB-I), Timosaponin B-II (TB-II), Timosaponin B-III (TB-III), and Timosaponin A-III (TA-III)^[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Molecular Weight

758.93

Formula

C₃₉H₆₆O₁₄

CAS No.

163047-21-0

Appearance

Solid

Color

White to off-white

SMILES

C[C@@]12[C@]([C@@H](O)[C@@]3([H])[C@]2([H])[C@@H](C(CCC(C)C)(O)O3)C)([H])[C@@]4([H])[C@]([C@@]5([C@](C[C@@H](O[C@@]6([H])[C@@H]([C@H]([C@@H](O)[C@@H](CO)O6)O)O[C@]7([H])O[C@@H]([C@@H](O)[C@H](O)[C@H]7O)CO)CC5)([H])CC4)C)([H])CC1

Structure Classification

Steroids

Initial Source

Shipping

Room temperature in continental US; may vary elsewhere.

Storage

4°C, protect from light

*In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)

Solvent & Solubility

In Vitro:

DMSO : ≥ 50 mg/mL (65.88 mM; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

*"≥" means soluble, but saturation unknown.

Preparing
Stock Solutions

Concentration Solvent Mass	1 mg	5 mg	10 mg

1 mM	1.3176 mL	6.5882 mL	13.1764 mL
5 mM	0.2635 mL	1.3176 mL	2.6353 mL
10 mM	0.1318 mL	0.6588 mL	1.3176 mL

View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Molarity Calculator
Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

This equation is commonly abbreviated as: C₁V₁ = C₂V₂

Concentration _(start)

Volume _(start)

Concentration _(final)

Volume _(final)

In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

Protocol 1

Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline
Solubility: ≥ 2.5 mg/mL (3.29 mM); Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.
Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
Protocol 2

Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in Saline)
Solubility: ≥ 2.5 mg/mL (3.29 mM); Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.
Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
Protocol 3

Add each solvent one by one: 10% DMSO 90% Corn Oil
Solubility: ≥ 2.5 mg/mL (3.29 mM); Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown). If the continuous dosing period exceeds half a month, please choose this protocol carefully.
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL Corn oil, and mix evenly.

In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Please enter your animal formula composition:

DMSO +

Tween-80 +

Saline

Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.

The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).

Calculation results:

Working solution concentration: mg/mL

Method for preparing stock solution: mg drug dissolved in μL DMSO (Stock solution concentration: mg/mL).

*In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)

The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only. If necessary, please contact MedChemExpress (MCE).

Method for preparing in vivo working solution for animal experiments: Take μL DMSO stock solution, add μL . μL , mix evenly, next add μL Tween 80, mix evenly, then add μL Saline.

If the continuous dosing period exceeds half a month, please choose this protocol carefully.

Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.

Purity & Documentation

Purity: 99.43%

Select Batch:

Data Sheet (274 KB) SDS (251 KB)

COA (253 KB) HNMR (327 KB) RP-HPLC (171 KB)

Handling Instructions (2659 KB)

References

[1]. Zhang J, et al. Effect of six steroidal saponins isolated from anemarrhenae rhizoma on platelet aggregation and hemolysis in human blood. Clin Chim Acta. 1999 Nov;289(1-2):79-88. [Content Brief]

Complete Stock Solution Preparation Table

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg

DMSO	1 mM	1.3176 mL	6.5882 mL	13.1764 mL	32.9411 mL
	5 mM	0.2635 mL	1.3176 mL	2.6353 mL	6.5882 mL
	10 mM	0.1318 mL	0.6588 mL	1.3176 mL	3.2941 mL
	15 mM	0.0878 mL	0.4392 mL	0.8784 mL	2.1961 mL
	20 mM	0.0659 mL	0.3294 mL	0.6588 mL	1.6471 mL
	25 mM	0.0527 mL	0.2635 mL	0.5271 mL	1.3176 mL
	30 mM	0.0439 mL	0.2196 mL	0.4392 mL	1.0980 mL
	40 mM	0.0329 mL	0.1647 mL	0.3294 mL	0.8235 mL
	50 mM	0.0264 mL	0.1318 mL	0.2635 mL	0.6588 mL
	60 mM	0.0220 mL	0.1098 mL	0.2196 mL	0.5490 mL