2. Autophagy Immunology/Inflammation Metabolic Enzyme/Protease NF-κB
  3. Autophagy Interleukin Related HIF/HIF Prolyl-Hydroxylase SOD NF-κB
  4. Astragaloside II

Astragaloside II  (Synonyms: Astrasieversianin VIII)

Cat. No.: HY-N0433 Purity: 99.45%
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Astragaloside II is an orally active Cycloartane-type triterpene glycoside. Astragaloside II can be extracted from Astragalus membranaceus. Astragaloside II inhibits Autophagy, decreases pro-inflammatory cytokines (IL-6, IL-1β), HIF-α, p-p65, p-IκB and increases SOD. Astragaloside II regulates immunity and reduces inflammatory responses. Astragaloside II can be used in the research of diseases such as liver cancer, osteoporosis, immunosuppressive diseases, and ulcerative colitis.

For research use only. We do not sell to patients.

Astragaloside II Chemical Structure

Astragaloside II Chemical Structure

CAS No. : 84676-89-1

Size Price Stock Quantity
Solid + Solvent (Highly Recommended)
10 mM * 1 mL in DMSO
ready for reconstitution
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Solution
10 mM * 1 mL in DMSO In-stock
Solid
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10 mg In-stock
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Customer Review

Based on 2 publication(s) in Google Scholar

Other Forms of Astragaloside II:

Astragaloside II Related Antibodies

Top Publications Citing Use of Products

1 Publications Citing Use of MCE Astragaloside II

Proliferation Assay

    Astragaloside II purchased from MedChemExpress. Usage Cited in: Am J Transl Res. 2019 Nov 15;11(11):7074-7083.  [Abstract]

    AS II (Astragaloside II) attenuates oxidative stress damage in LPS-stimulated CCD-18Co cells via decreasing the production of inflammatory factors. CCD-18Co cells are treated with LPS (1 μg/mL) for 0, 12, 24 and 48 h. In addition, CCD-18Co cells are treated with 1 μM AS II and 1 μg/mL LPS for 48 h. The level of IL-6 in the culture media is measured with ELISA. The level of TNF-α in the culture media is measured with ELISA. The level of IL-β in the culture media is measured with ELISA. CCD-18Co c

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    IL-1 IL-2 IL-3 IL-4 IL-5 IL-6 IL-8 IL-10 IL-12 IL-13 IL-15 IL-17 IL-23 IL-7 IL-33 IL-22

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    NF-κB NF-κB1/p50 RelA/p65 RelB c-Rel

    • Biological Activity

    • Purity & Documentation

    • References

    • Customer Review

    Description

    Astragaloside II is an orally active Cycloartane-type triterpene glycoside. Astragaloside II can be extracted from Astragalus membranaceus. Astragaloside II inhibits Autophagy, decreases pro-inflammatory cytokines (IL-6, IL-1β), HIF-α, p-p65, p-IκB and increases SOD. Astragaloside II regulates immunity and reduces inflammatory responses. Astragaloside II can be used in the research of diseases such as liver cancer, osteoporosis, immunosuppressive diseases, and ulcerative colitis[1][2][3][4][5].

    In Vitro

    Astragaloside II (0.1 nM-10 μM; 48-72 h) significantly promotes the cell viability of rat primary osteoblasts in a concentration-dependent manner[1].
    Astragaloside II (10-30 nM; 48-96 h) significantly enhances the proliferation of primary splenocytes induced by ConA, alloantigen or anti-CD3[3].
    Astragaloside II (80 μM; 48 h) sensitizes Bel-7402/FU cells to 5-fluorouracil (HY-90006)-induced cell death via suppression of autophagy[4].
    Astragaloside II (1 μM; 48 h) inhibits the levels of HIF-α, p-p65 and p-IκB in Lipopolysaccharide-stimulated CCD-18Co cells, exerting an anti-inflammatory effect[5].

    MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

    Astragaloside II Related Antibodies

    Western Blot Analysis[4]

    Cell Line: Bel-7402/FU
    Concentration: 40 μM, 80 μM, 160 μM, 320 μM, 80 μM
    Incubation Time: 48 h
    Result: Decreased the expression of autophagy-related proteins LC3-II and Beclin-1.

    Cell Viability Assay[5]

    Cell Line: CCD-18Co
    Concentration: 0.1 μM, 0.33 μM, 1 μM, 3 μM
    Incubation Time: 48 h
    Result: Significantly decreased the viability of CCD-18Co cells at 3 μM.
    Had very limited cytotoxicity at 1 μM.
    In Vivo

    Astragaloside II (30-50 mg/kg; p.o.; once per day; 10 days) alleviates the symptoms of DSS (HY-116282)-induced ulcerative colitis in mice[5].

    MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

    Animal Model: Male BABL/c mice (20-22 g) with DSS-induced ulcerative colitis[5]
    Dosage: 30 mg/kg (dissolved in 2% Tween 20 in PBS), 50 mg/kg (dissolved in 2% Tween 20 in PBS)
    Administration: Oral gavage, once per day, for 10 days
    Result: Significantly reduced the DAI score.
    Markedly prevented the body weight loss, increased the colon length, decreased the levels of pro-inflammatory cytokines IL-6, TNF-α, IL-1β, NO, MPO and MDA, and increased the level of SOD in colon tissues.
    Decreased the expression of inflammatory proteins HIF-α, p-p65 and p-IκB.
    Molecular Weight

    827.01

    Formula

    C43H70O15
    CAS No.
    Appearance

    Solid

    Color

    White to off-white

    SMILES

    O[C@H]1[C@H](O)[C@@H](OC(C)=O)[C@]([H])(O[C@@H]2C(C)(C)[C@@]([C@@H](O[C@]3([H])O[C@H](CO)[C@@H](O)[C@H](O)[C@H]3O)C[C@]4([H])[C@@]56CC[C@@]7(C)[C@@]4(C)C[C@H](O)[C@@]7([C@]8(C)O[C@H](C(O)(C)C)CC8)[H])([H])[C@]5(C6)CC2)OC1
    Structure Classification
    Initial Source
    Shipping

    Room temperature in continental US; may vary elsewhere.
    Storage
    Powder -20°C 3 years
    4°C 2 years
    In solvent -80°C 2 years
    -20°C 1 year
    Solvent & Solubility
    In Vitro: 

    DMSO : 150 mg/mL (181.38 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

    Preparing
    Stock Solutions
    Concentration Solvent Mass 1 mg 5 mg 10 mg
    1 mM 1.2092 mL 6.0459 mL 12.0918 mL
    5 mM 0.2418 mL 1.2092 mL 2.4184 mL
    View the Complete Stock Solution Preparation Table

    * Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
    Storage method and period of stock solution: -80°C, 2 years; -20°C, 1 year. When stored at -80°C, please use it within 2 years. When stored at -20°C, please use it within 1 year.

    • Molarity Calculator

    • Dilution Calculator

    Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

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    Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

    This equation is commonly abbreviated as: C1V1 = C2V2

    Concentration (start)

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    Volume (start)

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    In Vivo:

    Select the appropriate dissolution method based on your experimental animal and administration route.

    For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
    To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
    The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

    • Protocol 1

      Add each solvent one by one:  10% DMSO    40% PEG300    5% Tween-80    45% Saline

      Solubility: ≥ 2.5 mg/mL (3.02 mM); Clear solution

      This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).

      Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.

      Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
    • Protocol 2

      Add each solvent one by one:  10% DMSO    90% Corn Oil

      Solubility: ≥ 2.5 mg/mL (3.02 mM); Clear solution

      This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown). If the continuous dosing period exceeds half a month, please choose this protocol carefully.

      Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL Corn oil, and mix evenly.

    In Vivo Dissolution Calculator
    Please enter the basic information of animal experiments:

    Dosage

    mg/kg

    Animal weight
    (per animal)

    g

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    (per animal)

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    Number of animals

    Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
    Please enter your animal formula composition:
    %
    DMSO +
    % 
    +
    %
    Tween-80 +
    %
    Saline
    Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
    The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).
    Calculation results:
    Working solution concentration: mg/mL
    Method for preparing stock solution: mg drug dissolved in μL  DMSO (Stock solution concentration: mg/mL).
    The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only. If necessary, please contact MedChemExpress (MCE).
    Method for preparing in vivo working solution for animal experiments: Take μL DMSO stock solution, add μL . μL , mix evenly, next add μL Tween 80, mix evenly, then add μL Saline.
     If the continuous dosing period exceeds half a month, please choose this protocol carefully.
    Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.
    Purity & Documentation
    References

    Complete Stock Solution Preparation Table

    * Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
    Storage method and period of stock solution: -80°C, 2 years; -20°C, 1 year. When stored at -80°C, please use it within 2 years. When stored at -20°C, please use it within 1 year.

    Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
    DMSO 1 mM 1.2092 mL 6.0459 mL 12.0918 mL 30.2294 mL
    5 mM 0.2418 mL 1.2092 mL 2.4184 mL 6.0459 mL
    10 mM 0.1209 mL 0.6046 mL 1.2092 mL 3.0229 mL
    15 mM 0.0806 mL 0.4031 mL 0.8061 mL 2.0153 mL
    20 mM 0.0605 mL 0.3023 mL 0.6046 mL 1.5115 mL
    25 mM 0.0484 mL 0.2418 mL 0.4837 mL 1.2092 mL
    30 mM 0.0403 mL 0.2015 mL 0.4031 mL 1.0076 mL
    40 mM 0.0302 mL 0.1511 mL 0.3023 mL 0.7557 mL
    50 mM 0.0242 mL 0.1209 mL 0.2418 mL 0.6046 mL
    60 mM 0.0202 mL 0.1008 mL 0.2015 mL 0.5038 mL
    80 mM 0.0151 mL 0.0756 mL 0.1511 mL 0.3779 mL
    100 mM 0.0121 mL 0.0605 mL 0.1209 mL 0.3023 mL
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    Astragaloside II Related Classifications

    Help & FAQs
    • Do most proteins show cross-species activity?

      Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

    Keywords:

    Astragaloside II84676-89-1Astrasieversianin VIIIAutophagyInterleukin RelatedHIF/HIF Prolyl-HydroxylaseSODNF-κBILHypoxia-inducible factorsHIFsHIF-PHSuperoxide DismutaseNuclear factor-κBNuclear factor-kappaBosteoporosisimmunosuppressive diseasesulcerative colitismiceInhibitorinhibitorinhibit

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