BAPTA-AM

Name: BAPTA-AM
Brand: MedChemExpress
SKU: HY-100545
Rating: 5.0 (66 reviews)

Cat. No.: HY-100545 Purity: 99.68%: FAQs
Data Sheet SDS COA Handling Instructions

Molarity Calculator

BAPTA-AM is a well-known membrane permeable Ca²⁺ chelator. BAPTA-AM inhibits hERG channels, hKv1.3 and hKv1.5 channels in HEK 293 cells with IC₅₀s of 1.3 μM, 1.45 μM and 1.23 μM, respectively.

For research use only. We do not sell to patients.

BAPTA-AM Chemical Structure

CAS No. : 126150-97-8

Size	Price	Stock	Quantity

Free Sample (0.1 - 0.5 mg)		Apply Now
Solid + Solvent (Highly Recommended)
10 mM * 1 mL in DMSO ready for reconstitution	USD 55	In-stock	Estimated Time of Arrival: December 31
Solution
10 mM * 1 mL in DMSO	USD 55	In-stock	Estimated Time of Arrival: December 31
Solid
5 mg	USD 40	In-stock	Estimated Time of Arrival: December 31
10 mg	USD 65	In-stock	Estimated Time of Arrival: December 31
50 mg	USD 220	In-stock	Estimated Time of Arrival: December 31
100 mg		Get quote
200 mg		Get quote

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Customer Review

Based on 66 publication(s) in Google Scholar

63 Publications Citing Use of MCE BAPTA-AM

: BAPTA-AM purchased from MedChemExpress. Usage Cited in: Cell Stem Cell. 2022 Oct 12;S1934-5909(22)00417-9. [Abstract]; When HBMEC cells are pre-treated with BAPTA-AM (for 5, 15, 30, 60, 90 min), histamine-induced rapid activation of NF-kB signaling is significantly inhibited.

: BAPTA-AM purchased from MedChemExpress. Usage Cited in: Part Fibre Toxicol. 2018 Oct 19;15(1):39. [Abstract]; Total proteins of BV2 cells are extracted, and the levels of P₂X₇, NF-κB, ERK and p38 signaling pathway molecules are analyzed via Western Blot.

: BAPTA-AM purchased from MedChemExpress. Usage Cited in: Oncotarget. 2016 Feb 9;7(6):6711-26. [Abstract]; Under conditions of glucose limitation, BAPTA-AM treatment prevents HBx-induced phosphorylation of AMPK and ACC (A). This inhibitory effect is also observed in HBV-infected HepG2.2.15 cells in a concentration-dependent manner (B), implying that HBx-mediated mitochondrial calcium channels are required for activation of the AMPK pathway under such stressful conditions.

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Biological Activity
Protocol
Purity & Documentation
References
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Description

IC₅₀ & Target

Ca²⁺ chelator^[1]
IC50: 1.3 μM (hERG channel, in HEK 293 cells), 1.45 μM (hKv1.3, in HEK 293 cells), 1.23 μM (hKv1.5, in HEK 293 cells)^[1]

In Vitro

BAPTA-AM inhibits neuronal Ca²⁺-activated K⁺ channel currents, and up-regulates the decreased cardiac sodium current (INa) density by chelating intracellular Ca²⁺^[1].
BAPTA-AM (BAPTA/AM), an intracellular calcium chelator, induces delayed necrosis by lipoxygenase-mediated free radicals in mouse cortical cultures. BAPTA-AM prevents free radical-mediated toxicity promote apoptosis in non-neuronal cells and produce a beneficial effect in neuronal cells by protecting neurons from ischemic damage. In addition, it has been suggested that BAPTA-AM induces a late, but not early, increase of intracellular calcium in I-IL-60 neoplastic cells. Mixed cortical cell cultures (DIV 13-16) exposed to 10 μM BAPTA-AM for 24- or 48-hr show moderate (45-70%) neuronal injury as evaluated by increased LDH release into the bathing medium after 24-48-hr. Exposure of cortical cultures to 3-10 μM BAPTA-AM for 48-hr evoke dose-dependent neuronal damage^[2].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Molecular Weight

764.68

Formula

C₃₄H₄₀N₂O₁₈

CAS No.

126150-97-8

Appearance

Solid

Color

White to off-white

SMILES

O=C(OCOC(C)=O)CN(C(C=CC=C1)=C1OCCOC(C=CC=C2)=C2N(CC(OCOC(C)=O)=O)CC(OCOC(C)=O)=O)CC(OCOC(C)=O)=O

Shipping

Room temperature in continental US; may vary elsewhere.

Storage

Powder	-20°C	3 years
	4°C	2 years
In solvent	-80°C	2 years
	-20°C	1 year

Solvent & Solubility

In Vitro:

DMSO : 100 mg/mL (130.77 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Preparing
Stock Solutions

Concentration Solvent Mass	1 mg	5 mg	10 mg

1 mM	1.3077 mL	6.5387 mL	13.0774 mL
5 mM	0.2615 mL	1.3077 mL	2.6155 mL
10 mM	0.1308 mL	0.6539 mL	1.3077 mL

View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 2 years; -20°C, 1 year. When stored at -80°C, please use it within 2 years. When stored at -20°C, please use it within 1 year.

Molarity Calculator
Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

This equation is commonly abbreviated as: C₁V₁ = C₂V₂

Concentration _(start)

Volume _(start)

Concentration _(final)

Volume _(final)

In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

Protocol 1

Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline
Solubility: ≥ 2.5 mg/mL (3.27 mM); Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.
Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
Protocol 2

Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in Saline)
Solubility: 2.5 mg/mL (3.27 mM); Suspended solution; Need ultrasonic

This protocol yields a suspended solution of 2.5 mg/mL. Suspended solution can be used for oral and intraperitoneal injection.
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.
Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
Protocol 3

Add each solvent one by one: 10% DMSO 90% Corn Oil
Solubility: ≥ 2.5 mg/mL (3.27 mM); Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown). If the continuous dosing period exceeds half a month, please choose this protocol carefully.
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL Corn oil, and mix evenly.

In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Please enter your animal formula composition:

DMSO +

Tween-80 +

Saline

Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.

The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).

Calculation results:

Working solution concentration: mg/mL

Method for preparing stock solution: mg drug dissolved in μL DMSO (Stock solution concentration: mg/mL).

The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only. If necessary, please contact MedChemExpress (MCE).

Method for preparing in vivo working solution for animal experiments: Take μL DMSO stock solution, add μL . μL , mix evenly, next add μL Tween 80, mix evenly, then add μL Saline.

If the continuous dosing period exceeds half a month, please choose this protocol carefully.

Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.

Purity & Documentation

Purity: 99.68%

Select Batch:

Data Sheet (282 KB) SDS (251 KB)

COA (259 KB) RP-HPLC (245 KB)

Handling Instructions (2659 KB)

References

[1]. Tang Q, et al. The membrane permeable calcium chelator BAPTA-AM directly blocks human ether a-go-go-related gene potassium channels stably expressed in HEK 293 cells. Biochem Pharmacol. 2007 Dec 3;74(11):1596-607. [Content Brief]

[2]. Wie MB, et al. BAPTA/AM, an intracellular calcium chelator, induces delayed necrosis by lipoxygenase-mediated free radicals in mouse cortical cultures. Prog Neuropsychopharmacol Biol Psychiatry. 2001 Nov;25(8):1641-59. [Content Brief]

Cell Assay
^[1]

Neuronal injury is quantitatively estimated by measuring lactate dehydrogenase (LDH) released from damaged cells into the bathing medium 24- or 48-hr after the 10 μM BAPTA/AM treatment. The morphological findings are confirmed by staining with neuron-specific enolase (NSE) antibody and tryphan blue^[1].

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

References

[1]. Tang Q, et al. The membrane permeable calcium chelator BAPTA-AM directly blocks human ether a-go-go-related gene potassium channels stably expressed in HEK 293 cells. Biochem Pharmacol. 2007 Dec 3;74(11):1596-607. [Content Brief]

[2]. Wie MB, et al. BAPTA/AM, an intracellular calcium chelator, induces delayed necrosis by lipoxygenase-mediated free radicals in mouse cortical cultures. Prog Neuropsychopharmacol Biol Psychiatry. 2001 Nov;25(8):1641-59. [Content Brief]

Complete Stock Solution Preparation Table

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg

DMSO	1 mM	1.3077 mL	6.5387 mL	13.0774 mL	32.6934 mL
	5 mM	0.2615 mL	1.3077 mL	2.6155 mL	6.5387 mL
	10 mM	0.1308 mL	0.6539 mL	1.3077 mL	3.2693 mL
	15 mM	0.0872 mL	0.4359 mL	0.8718 mL	2.1796 mL
	20 mM	0.0654 mL	0.3269 mL	0.6539 mL	1.6347 mL
	25 mM	0.0523 mL	0.2615 mL	0.5231 mL	1.3077 mL
	30 mM	0.0436 mL	0.2180 mL	0.4359 mL	1.0898 mL
	40 mM	0.0327 mL	0.1635 mL	0.3269 mL	0.8173 mL
	50 mM	0.0262 mL	0.1308 mL	0.2615 mL	0.6539 mL
	60 mM	0.0218 mL	0.1090 mL	0.2180 mL	0.5449 mL
	80 mM	0.0163 mL	0.0817 mL	0.1635 mL	0.4087 mL
	100 mM	0.0131 mL	0.0654 mL	0.1308 mL	0.3269 mL

BAPTA-AM Related Classifications

Cardiovascular Disease

Help & FAQs

Do most proteins show cross-species activity?
Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

BAPTA-AM126150-97-8Potassium ChannelKcsAInhibitorinhibitorinhibit

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BAPTA-AM

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BAPTA-AM Related Antibodies

63 Publications Citing Use of MCE BAPTA-AM

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Complete Stock Solution Preparation Table

BAPTA-AM Related Classifications

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