Brofaromine (Synonyms: CGP 11305A)

Cat. No.: HY-13339 Purity: 99.60%: FAQs
Data Sheet SDS COA Handling Instructions

Molarity Calculator

Brofaromine (CGP 11305A) is a monoamine oxidase (MAO) inhibitor with IC₅₀ of 0.2 μM for MAO-A.

For research use only. We do not sell to patients.

Brofaromine Chemical Structure

CAS No. : 63638-91-5

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Other Forms of Brofaromine:

Brofaromine hydrochloride Get quote

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MAO-A MAO-B

Biological Activity
Purity & Documentation
References
Customer Review

Description

Brofaromine (CGP 11305A) is a monoamine oxidase (MAO) inhibitor with IC₅₀ of 0.2 μM for MAO-A.

IC₅₀ & Target

IC50: 0.2 μM (MAO-A)^[1]

In Vitro

In order to assess whether the protective effect is related to the inhibition of the MAO-A or due to another mechanism, the effect of Brofaromine (CGP 11305A, BRO) is determined on the activity of MAO-A in cultured cortical cells. It shows, firstly, that cultured cortical cells have a significant MAO-A activity, and secondly, that Brofaromine inhibits the enzyme in a concentration-dependent manner. The IC₅₀ is 0.19 μM for Brofaromine . Hill coefficient is 0.96. The enzyme is almost completely inhibited at 10 μM . Brofaromine inhibits MAO-A activity of our cultured cortical cells in a concentration-dependent manner. The IC₅₀ range of Brofaromine is 0.01 μM to 0.1 μM^[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Molecular Weight

310.19

Formula

C₁₄H₁₆BrNO₂

CAS No.

63638-91-5

Appearance

Solid

Color

Off-white to light yellow

SMILES

BrC1=CC(OC)=CC2=C1OC(C3CCNCC3)=C2

Shipping

Room temperature in continental US; may vary elsewhere.

Storage

Powder	-20°C	3 years
	4°C	2 years
In solvent	-80°C	2 years
	-20°C	1 year

Solvent & Solubility

In Vitro:

DMSO : 250 mg/mL (805.96 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Preparing
Stock Solutions

Concentration Solvent Mass	1 mg	5 mg	10 mg

1 mM	3.2238 mL	16.1192 mL	32.2383 mL
5 mM	0.6448 mL	3.2238 mL	6.4477 mL
10 mM	0.3224 mL	1.6119 mL	3.2238 mL

View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 2 years; -20°C, 1 year. When stored at -80°C, please use it within 2 years. When stored at -20°C, please use it within 1 year.

Molarity Calculator
Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

This equation is commonly abbreviated as: C₁V₁ = C₂V₂

Concentration _(start)

Volume _(start)

Concentration _(final)

Volume _(final)

In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

Protocol 1

Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline
Solubility: ≥ 6.25 mg/mL (20.15 mM); Clear solution

This protocol yields a clear solution of ≥ 6.25 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (62.5 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.
Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
Protocol 2

Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in Saline)
Solubility: ≥ 6.25 mg/mL (20.15 mM); Clear solution

This protocol yields a clear solution of ≥ 6.25 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (62.5 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.
Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
Protocol 3

Add each solvent one by one: 10% DMSO 90% Corn Oil
Solubility: ≥ 6.25 mg/mL (20.15 mM); Clear solution

This protocol yields a clear solution of ≥ 6.25 mg/mL (saturation unknown). If the continuous dosing period exceeds half a month, please choose this protocol carefully.
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (62.5 mg/mL) to 900 μL Corn oil, and mix evenly.

In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Please enter your animal formula composition:

DMSO +

Tween-80 +

Saline

Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.

The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).

Calculation results:

Working solution concentration: mg/mL

Method for preparing stock solution: mg drug dissolved in μL DMSO (Stock solution concentration: mg/mL).

The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only. If necessary, please contact MedChemExpress (MCE).

Method for preparing in vivo working solution for animal experiments: Take μL DMSO stock solution, add μL . μL , mix evenly, next add μL Tween 80, mix evenly, then add μL Saline.

If the continuous dosing period exceeds half a month, please choose this protocol carefully.

Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.

Purity & Documentation

Purity: 99.60%

Select Batch:

Data Sheet (270 KB) SDS (252 KB)

COA (245 KB) LCMS (92 KB)

Handling Instructions (2659 KB)

References

[1]. Boland A, et al. Pirlindole and dehydropirlindole protect rat cultured neuronal cells against oxidative stress-induced cell death through a mechanism unrelated to MAO-A inhibition. Br J Pharmacol. 2002 Feb;135(3):713-20. [Content Brief]

Complete Stock Solution Preparation Table

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg

DMSO	1 mM	3.2238 mL	16.1192 mL	32.2383 mL	80.5958 mL
	5 mM	0.6448 mL	3.2238 mL	6.4477 mL	16.1192 mL
	10 mM	0.3224 mL	1.6119 mL	3.2238 mL	8.0596 mL
	15 mM	0.2149 mL	1.0746 mL	2.1492 mL	5.3731 mL
	20 mM	0.1612 mL	0.8060 mL	1.6119 mL	4.0298 mL
	25 mM	0.1290 mL	0.6448 mL	1.2895 mL	3.2238 mL
	30 mM	0.1075 mL	0.5373 mL	1.0746 mL	2.6865 mL
	40 mM	0.0806 mL	0.4030 mL	0.8060 mL	2.0149 mL
	50 mM	0.0645 mL	0.3224 mL	0.6448 mL	1.6119 mL
	60 mM	0.0537 mL	0.2687 mL	0.5373 mL	1.3433 mL
	80 mM	0.0403 mL	0.2015 mL	0.4030 mL	1.0074 mL
	100 mM	0.0322 mL	0.1612 mL	0.3224 mL	0.8060 mL

Brofaromine Related Classifications

Neurological Disease

Help & FAQs

Do most proteins show cross-species activity?
Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

Brofaromine63638-91-5CGP 11305AMonoamine OxidaseMAOInhibitorinhibitorinhibit

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