2. Anti-infection
  3. Parasite Influenza Virus
  4. Broxyquinoline

Broxyquinoline  (Synonyms: Dibromohydroxyquinoline; 5,7-Dibromo-8-hydroxyquinoline)

Cat. No.: HY-B1212 Purity: 99.93%
SDS COA Handling Instructions

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Broxyquinoline (Dibromohydroxyquinoline) is an effective inhibitor of severe fever with thrombocytopenia syndrome virus (SFTSV) with an IC50 of 5.8 µM. Additionally, Broxyquinoline is an inhibitor of CpACBP1 with an IC50 of 64.9 μM. CpACBP1 is a fatty acyl-CoA binding protein of Cryptosporidium parvum, thus Broxyquinoline has the potential to inhibit parasite growth. Broxyquinoline shows promise for research in the field of infectious diseases.

For research use only. We do not sell to patients.

Broxyquinoline Chemical Structure

Broxyquinoline Chemical Structure

CAS No. : 521-74-4

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10 mM * 1 mL in DMSO
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Customer Review

Based on 1 publication(s) in Google Scholar

Broxyquinoline Related Antibodies

Top Publications Citing Use of Products

1 Publications Citing Use of MCE Broxyquinoline

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Amebae Coccidia Leishmania Mite Plasmodium Toxoplasma Trypanosoma Schistosome

  • Biological Activity

  • Purity & Documentation

  • References

  • Customer Review

Description

Broxyquinoline (Dibromohydroxyquinoline) is an effective inhibitor of severe fever with thrombocytopenia syndrome virus (SFTSV) with an IC50 of 5.8 µM. Additionally, Broxyquinoline is an inhibitor of CpACBP1 with an IC50 of 64.9 μM. CpACBP1 is a fatty acyl-CoA binding protein of Cryptosporidium parvum, thus Broxyquinoline has the potential to inhibit parasite growth. Broxyquinoline shows promise for research in the field of infectious diseases[1][2].

In Vitro

Broxyquinoline (1 mM, 10 μM, 44 h) has almost no active effect on Human ileocaecal colorectal adenocarcinoma (HCT-8) cells[1].
Broxyquinoline (0-1.0 mM, 44 h) exhibits antiparasitic activity in vitro[1].
Broxyquinoline (10 μM, 72 h) does not show significant toxicity to Vero cells and exhibits activity against severe fever with thrombocytopenia syndrome virus (SFTSV) [2].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Broxyquinoline Related Antibodies

Cell Viability Assay[1]

Cell Line: Human ileocaecal colorectal adenocarcinoma (HCT-8) cells
Concentration: 1 mM, 10 μM
Incubation Time: 44 h
Result: Did not exhibit inhibitory effects on Human ileocaecal colorectal adenocarcinoma (HCT-8) cells at both high concentration (1 mM) and low concentration (10 μM).

Cell Viability Assay[2]

Cell Line: Vero cells
Concentration: 10 μM
Incubation Time: 72 h
Result: Did not exhibit significant inhibitory effects on Vero cells.
In Vivo

A 5:1 mixture of broxyquinoline and broxaldin (0.1 g/kg, p.o., once daily for 32 days) did not cause any histological changes in the visceral organs of rats[3].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
Molecular Weight

302.95

Formula

C9H5Br2NO
CAS No.
Appearance

Solid

Color

Yellow to brown

SMILES

OC1=C2N=CC=CC2=C(Br)C=C1Br
Shipping

Room temperature in continental US; may vary elsewhere.
Storage

4°C, protect from light

*In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)

Solvent & Solubility
In Vitro: 

DMSO : 31.25 mg/mL (103.15 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

H2O : < 0.1 mg/mL (insoluble)

Preparing
Stock Solutions
Concentration Solvent Mass 1 mg 5 mg 10 mg
1 mM 3.3009 mL 16.5044 mL 33.0087 mL
5 mM 0.6602 mL 3.3009 mL 6.6017 mL
View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

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  • Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Mass
=
Concentration
×
Volume
×
Molecular Weight *

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start)

C1

×
Volume (start)

V1

=
Concentration (final)

C2

×
Volume (final)

V2

In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

  • Protocol 1

    Add each solvent one by one:  10% DMSO    40% PEG300    5% Tween-80    45% Saline

    Solubility: 2.08 mg/mL (6.87 mM); Suspended solution; Need ultrasonic

    This protocol yields a suspended solution of 2.08 mg/mL. Suspended solution can be used for oral and intraperitoneal injection.

    Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (20.8 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.

    Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
  • Protocol 2

    Add each solvent one by one:  10% DMSO    90% Corn Oil

    Solubility: ≥ 1.67 mg/mL (5.51 mM); Clear solution

    This protocol yields a clear solution of ≥ 1.67 mg/mL (saturation unknown). If the continuous dosing period exceeds half a month, please choose this protocol carefully.

    Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (16.7 mg/mL) to 900 μL Corn oil, and mix evenly.

In Vivo Dissolution Calculator
Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

g

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Please enter your animal formula composition:
%
DMSO +
% 
+
%
Tween-80 +
%
Saline
Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).
Calculation results:
Working solution concentration: mg/mL
Method for preparing stock solution: mg drug dissolved in μL  DMSO (Stock solution concentration: mg/mL).

*In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)

The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only. If necessary, please contact MedChemExpress (MCE).
Method for preparing in vivo working solution for animal experiments: Take μL DMSO stock solution, add μL . μL , mix evenly, next add μL Tween 80, mix evenly, then add μL Saline.
 If the continuous dosing period exceeds half a month, please choose this protocol carefully.
Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.
Purity & Documentation

Purity: 99.93%

SDS (393 KB)

COA (252 KB) HNMR (253 KB) LCMS (99 KB)

Handling Instructions (2659 KB)
References

Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
DMSO 1 mM 3.3009 mL 16.5044 mL 33.0087 mL 82.5219 mL
5 mM 0.6602 mL 3.3009 mL 6.6017 mL 16.5044 mL
10 mM 0.3301 mL 1.6504 mL 3.3009 mL 8.2522 mL
15 mM 0.2201 mL 1.1003 mL 2.2006 mL 5.5015 mL
20 mM 0.1650 mL 0.8252 mL 1.6504 mL 4.1261 mL
25 mM 0.1320 mL 0.6602 mL 1.3203 mL 3.3009 mL
30 mM 0.1100 mL 0.5501 mL 1.1003 mL 2.7507 mL
40 mM 0.0825 mL 0.4126 mL 0.8252 mL 2.0630 mL
50 mM 0.0660 mL 0.3301 mL 0.6602 mL 1.6504 mL
60 mM 0.0550 mL 0.2751 mL 0.5501 mL 1.3754 mL
80 mM 0.0413 mL 0.2063 mL 0.4126 mL 1.0315 mL
100 mM 0.0330 mL 0.1650 mL 0.3301 mL 0.8252 mL
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Broxyquinoline Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

Broxyquinoline521-74-4Dibromohydroxyquinoline 5,7-Dibromo-8-hydroxyquinolineParasiteInfluenza VirusHuman ileocaecal colorectal adenocarcinoma (HCT-8) cellsVero cellsAntiviral activityAntiparasitic activityInfectionInhibitorinhibitorinhibit

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