Bruceine A (Synonyms: Dihydrobrusatol; NSC310616)

Name: Bruceine A
Brand: MedChemExpress
SKU: HY-N0841
Rating: 5.0 (3 reviews)

Cat. No.: HY-N0841 Purity: 98.01%: FAQs
Data Sheet SDS COA Handling Instructions

Molarity Calculator

Bruceine A(NSC310616; Dihydrobrusatol) is a natural quassinoid compound extracted from the dried fruits of Brucea javanica (L.

For research use only. We do not sell to patients.

Bruceine A Chemical Structure

CAS No. : 25514-31-2

Size	Price	Stock	Quantity
Solid + Solvent (Highly Recommended)
10 mM * 1 mL in DMSO ready for reconstitution	USD 172	In-stock	Estimated Time of Arrival: December 31
Solution
10 mM * 1 mL in DMSO	USD 172	In-stock	Estimated Time of Arrival: December 31
Solid
5 mg	USD 150	In-stock	Estimated Time of Arrival: December 31
10 mg	USD 210	In-stock	Estimated Time of Arrival: December 31
25 mg	USD 390	In-stock	Estimated Time of Arrival: December 31
50 mg	USD 600	In-stock	Estimated Time of Arrival: December 31
100 mg		Get quote
200 mg		Get quote

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This product is a controlled substance and not for sale in your territory.

Customer Review

Based on 3 publication(s) in Google Scholar

3 Publications Citing Use of MCE Bruceine A

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Biological Activity
Purity & Documentation
References
Customer Review

Description

Bruceine A(NSC310616; Dihydrobrusatol) is a natural quassinoid compound extracted from the dried fruits of Brucea javanica (L.); are potential candidates for the treatment of canine babesiosis. IC50 value: Target: Bruceine A inhibited the in vitro growth of Babesia gibsoni in canine erythrocytes at lower concentration compared with the standard antibabesial drug diminazene aceturate and killed the parasites within 24 hr at a concentration of 25 nM. Oral administration of bruceine A at a dosage of 6.4 mg/kg/day for 5 days resulted in no clinical findings in a dog with normal ranges of hematological and biochemical values in the blood. Three dogs were infected with B. gibsoni and two of them were treated with bruceine A at a dosage of 6.4 mg/kg/day for 6 days from day 5 post-infection.

Cellular Effect

Cell Line	Type	Value	Description	References
HCT-116	IC₅₀	0.082 μM Compound: 9b	Antiproliferative activity against human HCT-116 cells incubated for 72 hrs by WST-1 assay Antiproliferative activity against human HCT-116 cells incubated for 72 hrs by WST-1 assay	[PMID: 33289552]
LNCaP	ED₅₀	96 nM Compound: 7	Cytotoxicity against hormone-dependent human LNCAP cells after 2 days by sulforhodamine B assay Cytotoxicity against hormone-dependent human LNCAP cells after 2 days by sulforhodamine B assay	[PMID: 19026551]
Lu1	ED₅₀	79 nM Compound: 7	Cytotoxicity against human Lu1 cells after 2 days by sulforhodamine B assay Cytotoxicity against human Lu1 cells after 2 days by sulforhodamine B assay	[PMID: 19026551]
MCF7	IC₅₀	0.18 μM Compound: 9b	Cytotoxicity against human MCF-7 cells incubated for 72 hrs by MTT assay Cytotoxicity against human MCF-7 cells incubated for 72 hrs by MTT assay	[PMID: 33289552]
MCF7	ED₅₀	1.5 nM Compound: 7	Cytotoxicity against human MCF7 cells after 2 days by sulforhodamine B assay in presence of 43 uM (-)-hydnocarpin Cytotoxicity against human MCF7 cells after 2 days by sulforhodamine B assay in presence of 43 uM (-)-hydnocarpin	[PMID: 19026551]
MCF7	ED₅₀	15 nM Compound: 7	Cytotoxicity against human MCF7 cells after 2 days by sulforhodamine B assay Cytotoxicity against human MCF7 cells after 2 days by sulforhodamine B assay	[PMID: 19026551]
MDA-MB-231	IC₅₀	0.23 μM Compound: 9b	Cytotoxicity against human MDA-MB-231 cells incubated for 72 hrs by MTT assay Cytotoxicity against human MDA-MB-231 cells incubated for 72 hrs by MTT assay	[PMID: 33289552]

Molecular Weight

522.54

Formula

C₂₆H₃₄O₁₁

CAS No.

25514-31-2

Appearance

Solid

Color

White to off-white

SMILES

O=C([C@@]1([C@H]([C@H](O)[C@]2([H])[C@]3(C4)C)O)[C@@]([C@H](C5=O)OC(CC(C)C)=O)([H])[C@@]2([C@@](O5)([H])C[C@@]3([H])C(C)=C(O)C4=O)CO1)OC

Structure Classification

Initial Source

Shipping

Room temperature in continental US; may vary elsewhere.

Storage

Powder	-20°C	3 years
	4°C	2 years
In solvent	-80°C	6 months
	-20°C	1 month

Solvent & Solubility

In Vitro:

DMSO : ≥ 25 mg/mL (47.84 mM; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

*"≥" means soluble, but saturation unknown.

Preparing
Stock Solutions

Concentration Solvent Mass	1 mg	5 mg	10 mg

1 mM	1.9137 mL	9.5686 mL	19.1373 mL
5 mM	0.3827 mL	1.9137 mL	3.8275 mL
10 mM	0.1914 mL	0.9569 mL	1.9137 mL

View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Molarity Calculator
Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

This equation is commonly abbreviated as: C₁V₁ = C₂V₂

Concentration _(start)

Volume _(start)

Concentration _(final)

Volume _(final)

In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

Protocol 1

Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline
Solubility: ≥ 2.08 mg/mL (3.98 mM); Clear solution

This protocol yields a clear solution of ≥ 2.08 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (20.8 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.
Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
Protocol 2

Add each solvent one by one: 10% DMSO 90% Corn Oil
Solubility: ≥ 2.08 mg/mL (3.98 mM); Clear solution

This protocol yields a clear solution of ≥ 2.08 mg/mL (saturation unknown). If the continuous dosing period exceeds half a month, please choose this protocol carefully.
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (20.8 mg/mL) to 900 μL Corn oil, and mix evenly.

In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Please enter your animal formula composition:

DMSO +

Tween-80 +

Saline

Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.

The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).

Calculation results:

Working solution concentration: mg/mL

Method for preparing stock solution: mg drug dissolved in μL DMSO (Stock solution concentration: mg/mL).

The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only. If necessary, please contact MedChemExpress (MCE).

Method for preparing in vivo working solution for animal experiments: Take μL DMSO stock solution, add μL . μL , mix evenly, next add μL Tween 80, mix evenly, then add μL Saline.

If the continuous dosing period exceeds half a month, please choose this protocol carefully.

Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.

Purity & Documentation

Purity: 98.01%

Select Batch:

Data Sheet (276 KB) SDS (251 KB)

COA (254 KB) HNMR (315 KB) LCMS (263 KB)

Handling Instructions (2659 KB)

References

[1]. Nakao R, et al. Evaluation of efficacy of bruceine A, a natural quassinoid compound extracted from a medicinal plant, Brucea javanica, for canine babesiosis. J Vet Med Sci. 2009 Jan;71(1):33-41. [Content Brief]

Complete Stock Solution Preparation Table

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg

DMSO	1 mM	1.9137 mL	9.5686 mL	19.1373 mL	47.8432 mL
	5 mM	0.3827 mL	1.9137 mL	3.8275 mL	9.5686 mL
	10 mM	0.1914 mL	0.9569 mL	1.9137 mL	4.7843 mL
	15 mM	0.1276 mL	0.6379 mL	1.2758 mL	3.1895 mL
	20 mM	0.0957 mL	0.4784 mL	0.9569 mL	2.3922 mL
	25 mM	0.0765 mL	0.3827 mL	0.7655 mL	1.9137 mL
	30 mM	0.0638 mL	0.3190 mL	0.6379 mL	1.5948 mL
	40 mM	0.0478 mL	0.2392 mL	0.4784 mL	1.1961 mL

Bruceine A Related Classifications

Infection

Help & FAQs

Do most proteins show cross-species activity?
Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

Bruceine A25514-31-2Dihydrobrusatol NSC310616NSC310616NSC 310616NSC-310616ParasiteAntibioticInhibitorinhibitorinhibit

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Bruceine A (Synonyms: Dihydrobrusatol; NSC310616)

Customer Review

Bruceine A Related Antibodies

3 Publications Citing Use of MCE Bruceine A

Featured Recommendations

•Related Screening Libraries:

•Related Small Molecules:

•Related Proteins:

View All Parasite Isoform Specific Products:

View All Antibiotic Isoform Specific Products:

Biological Activity

Purity & Documentation

References

Customer Review

Molarity Calculator

Dilution Calculator

Complete Stock Solution Preparation Table

Bruceine A Related Classifications

Keywords:

Your Recently Viewed Products:

Customer Review

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SAFETY DATA SHEET (SDS)

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