Chlorhexidine dihydrochloride

Name: Chlorhexidine dihydrochloride
Brand: MedChemExpress
SKU: HY-B1145
Rating: 5.0 (10 reviews)

Cat. No.: HY-B1145 Purity: 99.83%: FAQs
Data Sheet SDS COA Handling Instructions

Molarity Calculator

Chlorhexidine dihydrochloride is an orally active antimicrobial agent. Chlorhexidine dihydrochloride can damage the bacteria cell wall, inhibit proliferation of S. sobrinus and induce DNA damage.

For research use only. We do not sell to patients.

Chlorhexidine dihydrochloride Chemical Structure

CAS No. : 3697-42-5

Size	Price	Stock	Quantity

Free Sample (0.1 - 0.5 mg)		Apply Now
Solid + Solvent (Highly Recommended)
10 mM * 1 mL in DMSO ready for reconstitution	USD 33	In-stock	Estimated Time of Arrival: December 31
Solution
10 mM * 1 mL in DMSO	USD 33	In-stock	Estimated Time of Arrival: December 31
Solid
5 g	USD 30	In-stock	Estimated Time of Arrival: December 31
10 g	USD 43	In-stock	Estimated Time of Arrival: December 31
50 g		Get quote

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This product is a controlled substance and not for sale in your territory.

Customer Review

Based on 10 publication(s) in Google Scholar

Other Forms of Chlorhexidine dihydrochloride:

Chlorhexidine (digluconate) In-stock
Chlorhexidine In-stock
Chlorhexidine-d₈ dihydrochloride Get quote
Chlorhexidine acetate hydrate Get quote
Chlorhexidine diacetate Get quote
Chlorhexidine dihydrochloride (Standard) Get quote

9 Publications Citing Use of MCE Chlorhexidine dihydrochloride

: Chlorhexidine dihydrochloride purchased from MedChemExpress. Usage Cited in: Cancer Res. 2019 Feb 1;79(3):534-545. [Abstract]; The half-life of p53 protein is detected in GYS2 overexpressed cells by added with Chlorhexidine (CHX, 20 μg/mL) for different times.

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Biological Activity
Purity & Documentation
References
Customer Review

Description

Chlorhexidine dihydrochloride is an orally active antimicrobial agent. Chlorhexidine dihydrochloride can damage the bacteria cell wall, inhibit proliferation of S. sobrinus and induce DNA damage^[1]^[2]^[3].

In Vitro

Chlorhexidine dihydrochloride (100-200 μM, 15-240 min) causes clear dose-dependent reduction of S. aureus viability^[1].
Chlorhexidine dihydrochloride (1.25-20 μM, 24 h) inhibites the growth of human gingival fibroblast^[2].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Cell Viability Assay^[1]

Cell Line:	S. aureus
Concentration:	100 μM, 200 μM
Incubation Time:	15min, 30 min, 15 min, 60 min, 120 min, 240 min
Result:	Showed a slow decrease during the first two hours and a sharp drop between 2 and 4 h of incubation at 200 μM.

In Vivo

Chlorhexidine dihydrochloride (0.5 ml of 0.12% chlorhexidine digluconate, orally, twice daily for 8 days) caused DNA damage in rats^[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model:	Wistar rats^[1]
Dosage:	0.5 ml of 0.12% chlorhexidine digluconate
Administration:	Oral
Result:	Observed significant increase of DNA damage in leukocytes and oral mucosal cells.

Clinical Trial

Molecular Weight

578.37

Formula

C₂₂H₃₂Cl₄N₁₀

CAS No.

3697-42-5

Appearance

Solid

Color

White to off-white

SMILES

N=C(NC1=CC=C(Cl)C=C1)NC(NCCCCCCNC(NC(NC2=CC=C(Cl)C=C2)=N)=N)=N.[H]Cl.[H]Cl

Shipping

Room temperature in continental US; may vary elsewhere.

Storage

4°C, sealed storage, away from moisture

*In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)

Solvent & Solubility

In Vitro:

DMSO : 14 mg/mL (24.21 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Preparing
Stock Solutions

Concentration Solvent Mass	1 mg	5 mg	10 mg

1 mM	1.7290 mL	8.6450 mL	17.2900 mL
5 mM	0.3458 mL	1.7290 mL	3.4580 mL
10 mM	0.1729 mL	0.8645 mL	1.7290 mL

View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Molarity Calculator
Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

This equation is commonly abbreviated as: C₁V₁ = C₂V₂

Concentration _(start)

Volume _(start)

Concentration _(final)

Volume _(final)

In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

Protocol 1

Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline
Solubility: ≥ 2.08 mg/mL (3.60 mM); Clear solution

This protocol yields a clear solution of ≥ 2.08 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (20.8 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.
Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
Protocol 2

Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in Saline)
Solubility: ≥ 2.08 mg/mL (3.60 mM); Clear solution

This protocol yields a clear solution of ≥ 2.08 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (20.8 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.
Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
Protocol 3

Add each solvent one by one: 10% DMSO 90% Corn Oil
Solubility: ≥ 2.08 mg/mL (3.60 mM); Clear solution

This protocol yields a clear solution of ≥ 2.08 mg/mL (saturation unknown). If the continuous dosing period exceeds half a month, please choose this protocol carefully.
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (20.8 mg/mL) to 900 μL Corn oil, and mix evenly.

In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Please enter your animal formula composition:

DMSO +

Tween-80 +

Saline

Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.

The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).

Calculation results:

Working solution concentration: mg/mL

Method for preparing stock solution: mg drug dissolved in μL DMSO (Stock solution concentration: mg/mL).

*In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)

The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only. If necessary, please contact MedChemExpress (MCE).

Method for preparing in vivo working solution for animal experiments: Take μL DMSO stock solution, add μL . μL , mix evenly, next add μL Tween 80, mix evenly, then add μL Saline.

If the continuous dosing period exceeds half a month, please choose this protocol carefully.

Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.

Purity & Documentation

Purity: 99.83%

Select Batch:

Data Sheet (271 KB) SDS (393 KB)

COA (243 KB) HNMR (281 KB) LCMS (95 KB)

Handling Instructions (2659 KB)

References

[1]. Grigor'eva A, et al. Changes in the Ultrastructure of Staphylococcus aureus Treated with Cationic Peptides and Chlorhexidine. Microorganisms. 2020 Dec 14;8(12):1991. [Content Brief]

[2]. Dogan S, et al. Effects of low-concentrated chlorhexidine on growth of Streptococcus sobrinus and primary human gingival fibroblasts. Clin Oral Investig. 2003 Dec;7(4):212-6. [Content Brief]

[3]. Ribeiro DA, et al. Chlorhexidine induces DNA damage in rat peripheral leukocytes and oral mucosal cells. J Periodontal Res. 2004 Oct;39(5):358-61. [Content Brief]

Complete Stock Solution Preparation Table

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg

DMSO	1 mM	1.7290 mL	8.6450 mL	17.2900 mL	43.2249 mL
	5 mM	0.3458 mL	1.7290 mL	3.4580 mL	8.6450 mL
	10 mM	0.1729 mL	0.8645 mL	1.7290 mL	4.3225 mL
	15 mM	0.1153 mL	0.5763 mL	1.1527 mL	2.8817 mL
	20 mM	0.0864 mL	0.4322 mL	0.8645 mL	2.1612 mL