2. Apoptosis Anti-infection Autophagy Membrane Transporter/Ion Channel Neuronal Signaling
  3. Apoptosis Parasite Autophagy Caspase Calcium Channel
  4. Cinchonine

Cinchonine  (Synonyms: (8R,9S)-Cinchonine; LA40221)

Cat. No.: HY-Y0152 Purity: 99.56%
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Cinchonine is a natural compound present in Cinchona bark with antimalarial, antitumor, anti-inflammatory, anti platelet-aggregation and anti-obesity properties. Cinchonine inhibits cells proliferation and autophagy and induces apoptosis through activation of Caspase-3. Cinchonine activates endoplasmic reticulum stress-induced apoptosis in human liver cancer cells.

For research use only. We do not sell to patients.

Cinchonine Chemical Structure

Cinchonine Chemical Structure

CAS No. : 118-10-5

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Based on 2 publication(s) in Google Scholar

Other Forms of Cinchonine:

Cinchonine Related Antibodies

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N-type calcium channel L-type calcium channel P/Q-type calcium channel T-type calcium channel Calcium Channel

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Description

Cinchonine is a natural compound present in Cinchona bark with antimalarial, antitumor, anti-inflammatory, anti platelet-aggregation and anti-obesity properties. Cinchonine inhibits cells proliferation and autophagy and induces apoptosis through activation of Caspase-3. Cinchonine activates endoplasmic reticulum stress-induced apoptosis in human liver cancer cells[1].

Cellular Effect
Cell Line Type Value Description References
HeLa EC50
697 μg/mL
Compound: I
Cytotoxicity against human HeLa cells by MTT assay
Cytotoxicity against human HeLa cells by MTT assay
[PMID: 22846917]
WI-38 IC50
> 20 μg/mL
Compound: 1c
Cytotoxicity against human WI38 cells by MTT assay
Cytotoxicity against human WI38 cells by MTT assay
[PMID: 23816880]
WI-38 IC50
> 68.03 μM
Compound: CN
Cytotoxicity against human WI38 cells by MTT assay
Cytotoxicity against human WI38 cells by MTT assay
[PMID: 26063305]
In Vitro

Cinchonine induces apoptosis and inhibits cell proliferations in HepG2 and SMCC7721 (180 μM, 24 h), as well as in cells HeLa and A549 (180 μM, 48-96 h) [2][3].
Cinchonine (50-100 μM, 24 h) inihibits tumor growth in lungen cancer cells A549 and H1975, with IC50 values of 76.67 and 87.44 μM, respectively[4].
Cinchonine (0-20 μM, 24 h) blocks autophagy flux through the inhibition of a maturation of lysosomal hydrolases[4].
Cinchonine (20 μM) induces osteoclast differentiation and osteogenesis[6].
Cinchonine inhibits platelet aggregation through inhibition of Ca2+ flux (IC50: 300 μM) and protein kinase C (IC50: 20 μM)[7].
Cinchonine inhibits proliferation (0-100 μM, 72 h) of T. evansi , with IC50 of 16.96 μM in 24 h [8].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Cinchonine Related Antibodies

Western Blot Analysis[2][3][4]

Cell Line: HeLa, A549, HepG2, SMCC7721, H1975
Concentration: 180 μM (HeLa, A549, HepG2, SMCC7721), 200 μM (A549 and H1975)
Incubation Time: 24 h-96 h (HeLa and A549), 48 h (HepG2 and SMCC7721), 24 h (A549 and H1975)
Result: Inhibited cell proliferation in cells HeLa, A549, HepG2 and SMCC7721.
Cinchonine inhibited phosphorylation of AKT and TAK1.
Cinchonine activated Casapase-3, promoted GRP78 and phosphorylation of PERK and sIF2-α.
Cinchonine reduced mature cathepsin levels and increased immature cathespin levels.
In Vivo

Cinchonine (0.265-0.530 mg/kg, intratumorally injection, LLC cells for 14 days) induces cell apoptosis, suppresses tumor growth in BALB/c nude mice[2].
Cinchonine exhibits anti-metastatic activity in lung cancer cells with low toxicity[4].
Cinchonine(50mg/kg, i.p.) exhibits antiobesity activity in C57BL/6B mice[5].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model: BALB/c nude mice [4]
Dosage: 4 mg/kg/day
Administration: Intraperitoneal injection, for 19 days
Result: Inhibited metastatic activity
Animal Model: BALB/c nude mice [2]
Dosage: 0.265 and 0.530 mg/kg
Administration: Intratumorally injection, for 14 days
Result: Inhibited tumor growth with more TUNEL positive cells (DNA fragmentation indicator).
Animal Model: High-fat-diet (HFD) induced obesity in C57BL/6B mice[5]
Dosage: 50 mg/kg
Administration: Intraperitoneal injection
Result: Reduced body weight gain (−38%), visceral fat-pad weights (−26%).
Molecular Weight

294.39

Formula

C19H22N2O
CAS No.
Appearance

Solid

Color

White to off-white

SMILES

O[C@@H](C1=CC=NC2=CC=CC=C12)[C@]3([H])[N@@]4C[C@H](C=C)[C@@H](CC4)C3
Structure Classification
Initial Source
Shipping

Room temperature in continental US; may vary elsewhere.
Storage

4°C, protect from light, stored under nitrogen

*In solvent : -80°C, 6 months; -20°C, 1 month (protect from light, stored under nitrogen)

Solvent & Solubility
In Vitro: 

DMSO : 4.76 mg/mL (16.17 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Preparing
Stock Solutions
Concentration Solvent Mass 1 mg 5 mg 10 mg
1 mM 3.3969 mL 16.9843 mL 33.9685 mL
5 mM 0.6794 mL 3.3969 mL 6.7937 mL
View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light, stored under nitrogen). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

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Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

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Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

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In Vivo Dissolution Calculator
Please enter the basic information of animal experiments:

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Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Calculation results:
Working solution concentration: mg/mL
Purity & Documentation

Purity: 99.56%

SDS (393 KB)

COA (253 KB) HNMR (284 KB) LCMS (87 KB)

Handling Instructions (2659 KB)
References

Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light, stored under nitrogen). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
DMSO 1 mM 3.3969 mL 16.9843 mL 33.9685 mL 84.9214 mL
5 mM 0.6794 mL 3.3969 mL 6.7937 mL 16.9843 mL
10 mM 0.3397 mL 1.6984 mL 3.3969 mL 8.4921 mL
15 mM 0.2265 mL 1.1323 mL 2.2646 mL 5.6614 mL
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Cinchonine Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

Cinchonine118-10-5(8R,9S)-Cinchonine LA40221LA40221LA 40221LA-40221ApoptosisParasiteAutophagyCaspaseCalcium ChannelCa2+ channelsCa channelsanti platelet-aggregationanti-obesityantimalarialantitumoranti-inflammatoryBALB/c miceC57BL/6B miceA549HepG2SMCC7721H1975Inhibitorinhibitorinhibit

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