2. Anti-infection
  3. Bacterial
  4. D-(+)-Melezitose

D-(+)-Melezitose  (Synonyms: (+)-Melezitose; D-Melezitose)

Cat. No.: HY-N2340 Purity: ≥98.0%
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D-(+)-Melezitose can be used to identify clinical isolates of indole-positive and indole-negative Klebsiella spp.

For research use only. We do not sell to patients.

D-(+)-Melezitose Chemical Structure

D-(+)-Melezitose Chemical Structure

CAS No. : 597-12-6

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Solid + Solvent (Highly Recommended)
10 mM * 1 mL in DMSO
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10 mM * 1 mL in DMSO USD 66 In-stock
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Based on 1 publication(s) in Google Scholar

Other Forms of D-(+)-Melezitose:

D-(+)-Melezitose Related Antibodies

Top Publications Citing Use of Products

  • Biological Activity

  • Purity & Documentation

  • References

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Description

D-(+)-Melezitose can be used to identify clinical isolates of indole-positive and indole-negative Klebsiella spp.

In Vitro

A total of 102 (84%) of the 122 isolates are negative for indole production, unable to assimilate histamine and d-melezitose or to grow at 10°C. All of these 102 isolates are identified as possible K. pneumoniae/K. variicola. Finally, one isolate (1%) is positive for indole production, histamine assimilation, and growth at 10°C and negative for ornithine and d-melezitose. This isolate is identified as possible R. planticola[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

D-(+)-Melezitose Related Antibodies
Molecular Weight

504.44

Formula

C18H32O16
CAS No.
Appearance

Solid

Color

White to off-white

SMILES

OC[C@@]1([C@H]([C@H](O)[C@@H](CO)O1)O[C@@]2([H])O[C@@H]([C@@H](O)[C@H](O)[C@H]2O)CO)O[C@H]3O[C@@H]([C@@H](O)[C@H](O)[C@H]3O)CO
Structure Classification
Initial Source
Shipping

Room temperature in continental US; may vary elsewhere.
Storage
Powder -20°C 3 years
4°C 2 years
In solvent -80°C 2 years
-20°C 1 year
Solvent & Solubility
In Vitro: 

H2O : 100 mg/mL (198.24 mM; Need ultrasonic)

DMSO : 100 mg/mL (198.24 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Preparing
Stock Solutions
Concentration Solvent Mass 1 mg 5 mg 10 mg
1 mM 1.9824 mL 9.9120 mL 19.8240 mL
5 mM 0.3965 mL 1.9824 mL 3.9648 mL
View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 2 years; -20°C, 1 year. When stored at -80°C, please use it within 2 years. When stored at -20°C, please use it within 1 year.

* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

  • Molarity Calculator

  • Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Mass
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Concentration
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Volume
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Molecular Weight *

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start)

C1

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Volume (start)

V1

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Concentration (final)

C2

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Volume (final)

V2

In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

  • Protocol 1

    Add each solvent one by one:  10% DMSO    40% PEG300    5% Tween-80    45% Saline

    Solubility: ≥ 2.5 mg/mL (4.96 mM); Clear solution

    This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).

    Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.

    Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
  • Protocol 2

    Add each solvent one by one:  10% DMSO    90% (20% SBE-β-CD in Saline)

    Solubility: ≥ 2.5 mg/mL (4.96 mM); Clear solution

    This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).

    Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.

    Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.

For the following dissolution methods, please prepare the working solution directly. It is recommended to prepare fresh solutions and use them promptly within a short period of time.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

  • Protocol 1

    Add each solvent one by one:  PBS

    Solubility: 50 mg/mL (99.12 mM); Clear solution; Need ultrasonic and warming and heat to 60°C

In Vivo Dissolution Calculator
Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

g

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Calculation results:
Working solution concentration: mg/mL
This product has good water solubility, please refer to the measured solubility data in water/PBS/Saline for details.
The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only.If necessary, please contact MedChemExpress (MCE).
Purity & Documentation
References

Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 2 years; -20°C, 1 year. When stored at -80°C, please use it within 2 years. When stored at -20°C, please use it within 1 year.

Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
H2O / DMSO 1 mM 1.9824 mL 9.9120 mL 19.8240 mL 49.5599 mL
5 mM 0.3965 mL 1.9824 mL 3.9648 mL 9.9120 mL
10 mM 0.1982 mL 0.9912 mL 1.9824 mL 4.9560 mL
15 mM 0.1322 mL 0.6608 mL 1.3216 mL 3.3040 mL
20 mM 0.0991 mL 0.4956 mL 0.9912 mL 2.4780 mL
25 mM 0.0793 mL 0.3965 mL 0.7930 mL 1.9824 mL
30 mM 0.0661 mL 0.3304 mL 0.6608 mL 1.6520 mL
40 mM 0.0496 mL 0.2478 mL 0.4956 mL 1.2390 mL
50 mM 0.0396 mL 0.1982 mL 0.3965 mL 0.9912 mL
60 mM 0.0330 mL 0.1652 mL 0.3304 mL 0.8260 mL
80 mM 0.0248 mL 0.1239 mL 0.2478 mL 0.6195 mL
100 mM 0.0198 mL 0.0991 mL 0.1982 mL 0.4956 mL

* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

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D-(+)-Melezitose Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

D-(+)-Melezitose597-12-6(+)-Melezitose D-MelezitoseBacterialInhibitorinhibitorinhibit

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