Dacarbazine (Synonyms: Imidazole Carboxamide)

Name: Dacarbazine
Brand: MedChemExpress
SKU: HY-B0078
Rating: 5.0 (26 reviews)

Cat. No.: HY-B0078 Purity: 99.96%: FAQs
Data Sheet SDS COA Handling Instructions

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Dacarbazine is a nonspecific antineoplastic (antineoplastic) alkylating agent. Dacarbazine inhibits T and B lymphocyte responses with IC₅₀ of 50 and 10 μg/mL, respectively. Dacarbazine can be used in the study of metastatic malignant melanoma.

For research use only. We do not sell to patients.

Dacarbazine Chemical Structure

CAS No. : 4342-03-4

Size	Price	Stock	Quantity
Solid + Solvent (Highly Recommended)
10 mM * 1 mL in DMSO ready for reconstitution	USD 87	In-stock	Estimated Time of Arrival: December 31
Solution
10 mM * 1 mL in DMSO	USD 87	In-stock	Estimated Time of Arrival: December 31
Solid
50 mg	USD 38	In-stock	Estimated Time of Arrival: December 31
100 mg	USD 60	In-stock	Estimated Time of Arrival: December 31
200 mg	USD 79	In-stock	Estimated Time of Arrival: December 31
1 g	USD 132	In-stock	Estimated Time of Arrival: December 31
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10 g		Get quote

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Based on 26 publication(s) in Google Scholar

Other Forms of Dacarbazine:

Bismuth potassium citrate In-stock
Lithium citrate tetrahydrate In-stock
Sodium citrate dihydrate In-stock
Citric acid In-stock
Dacarbazine-d₆ Get quote
Dacarbazine citrate Get quote
Dacarbazine hydrochloride Get quote
Ammonium citrate dibasic, 99% Get quote
Citric acid triammonium Get quote
Sodium citrate dihydrate, meets USP testing specifications Get quote
Sodium citrate dihydrate, United States Pharmacopeia (USP) Reference Standard Get quote
Sodium citrate dihydrate, for molecular biology Get quote
Ammonium iron(III) citrate Get quote
Lithium citrate Get quote
Citric acid trisodium Get quote
Citric acid monohydrate Get quote
Hydroxycitric acid Get quote
Citric acid tripotassium hydrate Get quote
Bismuth(Ⅲ) citrate, 99.99% trace metals basis Get quote
Dacarbazine (Standard) Get quote

26 Publications Citing Use of MCE Dacarbazine

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Biological Activity
Purity & Documentation
References
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Description

Cellular Effect

Cell Line	Type	Value	Description	References
A-375	IC₅₀	> 100 μM Compound: DTIC	Antiproliferative activity against human A375 cells after 48 hrs by SRB assay Antiproliferative activity against human A375 cells after 48 hrs by SRB assay	[PMID: 20056548]
A-375	IC₅₀	> 100 μM Compound: DTIC, dacarbazine	Antiproliferative activity against human A375 cells after 48 hrs by SRB assay Antiproliferative activity against human A375 cells after 48 hrs by SRB assay	[PMID: 18477505]
A-375	IC₅₀	> 500 μM Compound: dacarbazine	Cytotoxicity against human A375 cells after 48 hrs by MTT assay Cytotoxicity against human A375 cells after 48 hrs by MTT assay	[PMID: 22560315]
A549	IC₅₀	35 μg/mL Compound: Dacarbazine	Cytotoxicity against Homo sapiens (human) A549 cells assessed as cell viability by MTT assay Cytotoxicity against Homo sapiens (human) A549 cells assessed as cell viability by MTT assay	10.1007/s00044-007-9031-x
B16-F1	IC₅₀	> 100 μM Compound: DTIC	Antiproliferative activity against mouse B16-F1 cells after 48 hrs by SRB assay Antiproliferative activity against mouse B16-F1 cells after 48 hrs by SRB assay	[PMID: 20056548]
B16-F1	IC₅₀	> 100 μM Compound: DTIC, dacarbazine	Antiproliferative activity against mouse B16-F1 cells after 48 hrs by SRB assay Antiproliferative activity against mouse B16-F1 cells after 48 hrs by SRB assay	[PMID: 18477505]
Fibroblast	IC₅₀	> 100 μM Compound: DTIC	Cytotoxicity against human fibroblasts after 48 hrs by SRB assay Cytotoxicity against human fibroblasts after 48 hrs by SRB assay	[PMID: 20056548]
HeLa	GI₅₀	> 50 μM Compound: DTIC	Cytotoxicity against human HeLa cells assessed as reduction in cell viability after 24 to 48 hrs by MTT assay Cytotoxicity against human HeLa cells assessed as reduction in cell viability after 24 to 48 hrs by MTT assay	[PMID: 24986661]
OVCAR-3	IC₅₀	17.67 μM Compound: Dacarbazine	Cytotoxicity against human OVCAR3 cells after 6 days by MTT assay Cytotoxicity against human OVCAR3 cells after 6 days by MTT assay	[PMID: 21131205]
SK-MEL-2	IC₅₀	120 μM Compound: Dacarbazine	Cytotoxicity against human SK-MEL-2 cells after 6 days by MTT assay Cytotoxicity against human SK-MEL-2 cells after 6 days by MTT assay	[PMID: 21131205]
UACC-62	IC₅₀	11.83 μM Compound: Dacarbazine	Cytotoxicity against human UACC62 cells after 6 days by MTT assay Cytotoxicity against human UACC62 cells after 6 days by MTT assay	[PMID: 21131205]
WM164	IC₅₀	> 100 μM Compound: DTIC	Antiproliferative activity against human WM164 cells after 48 hrs by SRB assay Antiproliferative activity against human WM164 cells after 48 hrs by SRB assay	[PMID: 20056548]
WM164	IC₅₀	> 100 μM Compound: DTIC	Antiproliferative activity against human WM164 cells after 48 hrs by SRB assay Antiproliferative activity against human WM164 cells after 48 hrs by SRB assay	[PMID: 17561392]

In Vitro

Dacarbazine (6.25-500 μg/mL 48 h) combines with hyperthermia-induced cytotoxicity of A375 and MNT-1 melanoma cells^[5].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Cell Viability Assay^[5]

Cell Line:	A375, MNT-1
Concentration:	6.25, 12.5, 25, 50, 100, 200, 400, 500 μg/mL
Incubation Time:	24, 48, 72 h
Result:	Inhibited cell viability in a concentration-dependent manner.

Cell Cycle Analysis^[5]

Cell Line:	A375, MNT-1
Concentration:	5.5, 115 μg/mL
Incubation Time:	48 h
Result:	Decreased (9.3%) the percentage of A375 cells at the S phase and increased the number of cells at G2/M. Decreased MNT-1 cells at G0/G1, increasing in cells at both S and G2/M phases.

Clinical Trial

Molecular Weight

182.18

Formula

C₆H₁₀N₆O

CAS No.

4342-03-4

Appearance

Solid

Color

White to yellow

SMILES

O=C(C1=C(/N=N/N(C)C)NC=N1)N

Shipping

Room temperature in continental US; may vary elsewhere.

Storage

4°C, protect from light

*In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)

Solvent & Solubility

In Vitro:

0.1 M HCL : 20 mg/mL (109.78 mM; ultrasonic and warming and heat to 60°C)

DMSO : 5 mg/mL (27.45 mM; ultrasonic and warming and heat to 60°C; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

H₂O : < 0.1 mg/mL (insoluble)

Preparing
Stock Solutions

Concentration Solvent Mass	1 mg	5 mg	10 mg

1 mM	5.4891 mL	27.4454 mL	54.8908 mL
5 mM	1.0978 mL	5.4891 mL	10.9782 mL
10 mM	0.5489 mL	2.7445 mL	5.4891 mL

View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Molarity Calculator
Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

This equation is commonly abbreviated as: C₁V₁ = C₂V₂

Concentration _(start)

Volume _(start)

Concentration _(final)

Volume _(final)

In Vivo:

For the following dissolution methods, please prepare the working solution directly. It is recommended to prepare fresh solutions and use them promptly within a short period of time.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

Protocol 1

Add each solvent one by one: PBS
Solubility: 2 mg/mL (10.98 mM); Clear solution; Need ultrasonic and warming and heat to 60°C
Protocol 2

Add each solvent one by one: 50% PEG300 50% Saline
Solubility: 5 mg/mL (27.45 mM); Suspended solution; Need ultrasonic

In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Calculation results:

Working solution concentration: mg/mL

Purity & Documentation

Purity: 99.96%

Select Batch:

Data Sheet (279 KB) SDS (419 KB)

COA (254 KB) HNMR (252 KB) RP-HPLC (257 KB) MS (206 KB)

Handling Instructions (2659 KB)

References

[1]. Serrone, L., et al., Dacarbazine-based chemotherapy for metastatic melanoma: thirty-year experience overview. J Exp Clin Cancer Res, 2000. 19(1): p. 21-34. [Content Brief]

[2]. Al-Badr AA, et al. Dacarbazine. Profiles Drug Subst Excip Relat Methodol. 2016;41:323-77. [Content Brief]

[3]. Rojo JM, et al. Inhibition of T and B lymphoblastic response by mithramycin, dacarbazine, prospidium chloride and peptichemio. Chemotherapy. 1983;29(5):345-51. [Content Brief]

[4]. Erdmann S, et al. Induced cross-resistance of BRAFV600E melanoma cells to standard chemotherapeutic dacarbazine after chronic PLX4032 treatment. Sci Rep. 2019 Jan 10;9(1):30. [Content Brief]

[5]. Salvador D, et al. Combined Therapy with Dacarbazine and Hyperthermia Induces Cytotoxicity in A375 and MNT-1 Melanoma Cells. Int J Mol Sci. 2022 Mar 25;23(7):3586. [Content Brief]

Complete Stock Solution Preparation Table

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg

DMSO / 0.1 M HCL	1 mM	5.4891 mL	27.4454 mL	54.8908 mL	137.2269 mL
	5 mM	1.0978 mL	5.4891 mL	10.9782 mL	27.4454 mL
	10 mM	0.5489 mL	2.7445 mL	5.4891 mL	13.7227 mL
	15 mM	0.3659 mL	1.8297 mL	3.6594 mL	9.1485 mL
	20 mM	0.2745 mL	1.3723 mL	2.7445 mL	6.8613 mL
	25 mM	0.2196 mL	1.0978 mL	2.1956 mL	5.4891 mL
0.1 M HCL	30 mM	0.1830 mL	0.9148 mL	1.8297 mL	4.5742 mL
	40 mM	0.1372 mL	0.6861 mL	1.3723 mL	3.4307 mL
	50 mM	0.1098 mL	0.5489 mL	1.0978 mL	2.7445 mL
	60 mM	0.0915 mL	0.4574 mL	0.9148 mL	2.2871 mL
	80 mM	0.0686 mL	0.3431 mL	0.6861 mL	1.7153 mL
	100 mM	0.0549 mL	0.2745 mL	0.5489 mL	1.3723 mL

Dacarbazine Related Classifications

Help & FAQs

Do most proteins show cross-species activity?
Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

Dacarbazine4342-03-4Imidazole CarboxamideNucleoside Antimetabolite/AnalogApoptosisMelanomaA375MNT-1AntitumorInhibitorinhibitorinhibit

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Dacarbazine (Synonyms: Imidazole Carboxamide)

Customer Review

Other Forms of Dacarbazine:

Dacarbazine Related Antibodies

26 Publications Citing Use of MCE Dacarbazine

Featured Recommendations

•Related Screening Libraries:

•Related Small Molecules:

•Related Proteins:

Biological Activity

Purity & Documentation

References

Customer Review

Dacarbazine Related Antibodies

Molarity Calculator

Dilution Calculator

Complete Stock Solution Preparation Table

Dacarbazine Related Classifications

Keywords:

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