Echinocystic acid

Name: Echinocystic acid
Brand: MedChemExpress
SKU: HY-N0271
Rating: 4.5 (1 reviews)

Cat. No.: HY-N0271 Purity: 98.10%: FAQs
Data Sheet SDS COA Handling Instructions

Molarity Calculator

Echinocystic acid is a natural pentacyclic triterpene with potent antioxidant, anti-inflammatory and analgesic activities.

For research use only. We do not sell to patients.

Echinocystic acid Chemical Structure

CAS No. : 510-30-5

Size	Price	Stock	Quantity
Solid + Solvent (Highly Recommended)
10 mM * 1 mL in DMSO ready for reconstitution	USD 31	In-stock	Estimated Time of Arrival: December 31
Solution
10 mM * 1 mL in DMSO	USD 31	In-stock	Estimated Time of Arrival: December 31
Solid
5 mg	USD 30	In-stock	Estimated Time of Arrival: December 31
10 mg	USD 45	In-stock	Estimated Time of Arrival: December 31
25 mg	USD 85	In-stock	Estimated Time of Arrival: December 31
50 mg		Get quote
100 mg		Get quote

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Customer Review

Based on 1 publication(s) in Google Scholar

Other Forms of Echinocystic acid:

Echinocystic acid (Standard) Get quote

1 Publications Citing Use of MCE Echinocystic acid

•SSRN. 2024 Feb 23.

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Biological Activity
Purity & Documentation
References
Customer Review

Description

Echinocystic acid is a natural pentacyclic triterpene with potent antioxidant, anti-inflammatory and analgesic activities^[1]^[2].

Cellular Effect

Cell Line	Type	Value	Description	References
HT-29	EC₅₀	> 10 μM Compound: 9	Anti-necroptotic activity in human HT-29 cells assessed as inhibition of TNFalpha/SM-164/Z-VAD-fmk (TSZ)-induced necroptosis by measuring increase in cell viability measured after 12 hrs by celltiter-glo luminescent cell viability assay Anti-necroptotic activity in human HT-29 cells assessed as inhibition of TNFalpha/SM-164/Z-VAD-fmk (TSZ)-induced necroptosis by measuring increase in cell viability measured after 12 hrs by celltiter-glo luminescent cell viability assay	[PMID: 33248849]
MDCK	CC₅₀	15.41 μM Compound: EA	Cytotoxicity against MDCK cells measured after 48 hrs by alamar blue assay Cytotoxicity against MDCK cells measured after 48 hrs by alamar blue assay	[PMID: 27565552]
SK-OV-3	IC₅₀	21.47 μM Compound: Echinocystic acid	Reversal of platinum resistance in cisplatin-resistant human SK-OV-3 cells assessed as reduction in cisplatin IC50 after 48 hrs in presence of cisplatin by SRB assay (Rvb = 28.23 microM) Reversal of platinum resistance in cisplatin-resistant human SK-OV-3 cells assessed as reduction in cisplatin IC50 after 48 hrs in presence of cisplatin by SRB assay (Rvb = 28.23 microM)	[PMID: 35500202]
SK-OV-3	IC₅₀	96.45 μM Compound: Echinocystic acid	Cytotoxicity against human SK-OV-3 cells assessed as reduction in cell viability incubated for 48 hrs by SRB assay Cytotoxicity against human SK-OV-3 cells assessed as reduction in cell viability incubated for 48 hrs by SRB assay	[PMID: 35500202]

In Vitro

Echinocystic acid (2.5-10 μM; pretreated 1 h) inhibits IL-1β-induced NF-κB and MAPK activation^[1].
Echinocystic acid suppresses IL-1β-induced MMP-13, NO, and PGE2 production in a dose-dependent manner. IL-1β up-regulated the expression of COX-2 and iNOS, and the increase is inhibited by Echinocystic acid^[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Western Blot Analysis^[1]

Cell Line:	Chondrocytes
Concentration:	2.5 μM, 5 μM, 10 μM
Incubation Time:	Pretreated 1 h and then stimulated with IL-1β for 30 min.
Result:	IL-1β-induced NF-κB and MAPK activation were inhibited.

In Vivo

Echinocystic acid (5 mg/kg intragastrically daily for 5 days) shows anti-depression activities in the mouse model of reserpine-induced pain-depression dyad^[2].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model:	Male adult C57BL/6 mice (8-10 week old; 18 to 22 g) injected weith Reserpine^[2]
Dosage:	5 mg/kg
Administration:	Intragastrically; daily for 5 days
Result:	Attenuated reserpine-induced pain/depression dyad partially through regulating the biogenic amines levels and GluN2B receptors in the hippocampus.

Molecular Weight

472.70

Formula

C₃₀H₄₈O₄

CAS No.

510-30-5

Appearance

Solid

Color

White to off-white

SMILES

CC1(C)CC[C@]2(C(O)=O)[C@H](O)C[C@@]3(C)[C@]4(C)CC[C@@]5([H])C(C)(C)[C@@H](O)CC[C@]5(C)[C@@]4([H])CC=C3[C@]2([H])C1

Structure Classification

Initial Source

Shipping

Room temperature in continental US; may vary elsewhere.

Storage

Powder	-20°C	3 years
	4°C	2 years
In solvent	-80°C	2 years
	-20°C	1 year

Solvent & Solubility

In Vitro:

DMSO : 50 mg/mL (105.78 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Preparing
Stock Solutions

Concentration Solvent Mass	1 mg	5 mg	10 mg

1 mM	2.1155 mL	10.5775 mL	21.1551 mL
5 mM	0.4231 mL	2.1155 mL	4.2310 mL
10 mM	0.2116 mL	1.0578 mL	2.1155 mL

View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 2 years; -20°C, 1 year. When stored at -80°C, please use it within 2 years. When stored at -20°C, please use it within 1 year.

Molarity Calculator
Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

This equation is commonly abbreviated as: C₁V₁ = C₂V₂

Concentration _(start)

Volume _(start)

Concentration _(final)

Volume _(final)

In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

Protocol 1

Add each solvent one by one: 10% DMSO 90% Corn Oil
Solubility: ≥ 2.75 mg/mL (5.82 mM); Clear solution

This protocol yields a clear solution of ≥ 2.75 mg/mL (saturation unknown). If the continuous dosing period exceeds half a month, please choose this protocol carefully.
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (27.5 mg/mL) to 900 μL Corn oil, and mix evenly.

In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Please enter your animal formula composition:

DMSO +

Tween-80 +

Saline

Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.

The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).

Calculation results:

Working solution concentration: mg/mL

Method for preparing stock solution: mg drug dissolved in μL DMSO (Stock solution concentration: mg/mL).

The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only. If necessary, please contact MedChemExpress (MCE).

Method for preparing in vivo working solution for animal experiments: Take μL DMSO stock solution, add μL . μL , mix evenly, next add μL Tween 80, mix evenly, then add μL Saline.

If the continuous dosing period exceeds half a month, please choose this protocol carefully.

Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.

Purity & Documentation

Purity: 98.10%

Select Batch:

Data Sheet (277 KB) SDS (252 KB)

COA (252 KB) HNMR (271 KB) LCMS (89 KB)

Handling Instructions (2659 KB)

References

[1]. Ma Z et al. Echinocystic Acid Inhibits IL-1β-Induced COX-2 and iNOS Expression in Human Osteoarthritis Chondrocytes. Inflammation. 2016 Apr;39(2):543-9. [Content Brief]

[2]. Li S et al. Echinocystic acid reduces reserpine-induced pain/depression dyad in mice. Metab Brain Dis. 2016 Apr;31(2):455-63. [Content Brief]

Complete Stock Solution Preparation Table

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg

DMSO	1 mM	2.1155 mL	10.5775 mL	21.1551 mL	52.8877 mL
	5 mM	0.4231 mL	2.1155 mL	4.2310 mL	10.5775 mL
	10 mM	0.2116 mL	1.0578 mL	2.1155 mL	5.2888 mL
	15 mM	0.1410 mL	0.7052 mL	1.4103 mL	3.5258 mL
	20 mM	0.1058 mL	0.5289 mL	1.0578 mL	2.6444 mL
	25 mM	0.0846 mL	0.4231 mL	0.8462 mL	2.1155 mL
	30 mM	0.0705 mL	0.3526 mL	0.7052 mL	1.7629 mL
	40 mM	0.0529 mL	0.2644 mL	0.5289 mL	1.3222 mL
	50 mM	0.0423 mL	0.2116 mL	0.4231 mL	1.0578 mL
	60 mM	0.0353 mL	0.1763 mL	0.3526 mL	0.8815 mL
	80 mM	0.0264 mL	0.1322 mL	0.2644 mL	0.6611 mL
	100 mM	0.0212 mL	0.1058 mL	0.2116 mL	0.5289 mL