Endothelin 1 (swine, human)

Name: Endothelin 1 (swine, human)
Brand: MedChemExpress
SKU: HY-P0202
Rating: 5.0 (6 reviews)

Cat. No.: HY-P0202 Purity: 98.60%: FAQs
Data Sheet SDS COA Handling Instructions

Molarity Calculator

Endothelin 1 (swine, human) is a synthetic peptide with the sequence of human and swine Endothelin 1, which is a potent endogenous vasoconstrictor. Endothelin 1 acts through two types of receptors ET_A and ET_B.

For research use only. We do not sell to patients.

Custom Peptide Synthesis

Endothelin 1 (swine, human) Chemical Structure

CAS No. : 117399-94-7

Size	Price	Stock	Quantity
500 μg	USD 144	In-stock	Estimated Time of Arrival: December 31
1 mg	USD 264	In-stock	Estimated Time of Arrival: December 31
5 mg	USD 850	In-stock	Estimated Time of Arrival: December 31
10 mg	USD 1360	In-stock	Estimated Time of Arrival: December 31
50 mg		Get quote
100 mg		Get quote

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Customer Review

Based on 6 publication(s) in Google Scholar

Other Forms of Endothelin 1 (swine, human):

Endothelin 1 (swine, human) (TFA) Get quote

6 Publications Citing Use of MCE Endothelin 1 (swine, human)

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View All Endothelin Receptor Isoform Specific Products:

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ET_A ET_B

Biological Activity
Purity & Documentation
References
Customer Review

Description

IC₅₀ & Target

ET_A

ET_B

In Vitro

Endothelin-1 (ET-1) is a potent endogenous vasoconstrictor, mainly secreted by endothelial cells. It acts through two types of receptors: ETA and ETB. Apart from a vasoconstrictive action, ET-1 causes fibrosis of the vascular cells and stimulates production of reactive oxygen species. It is claimed that ET-1 induces proinflammatory mechanisms, increasing superoxide anion production and cytokine secretion^[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Molecular Weight

2491.90

Formula

C₁₀₉H₁₅₉N₂₅O₃₂S₅

CAS No.

117399-94-7

Appearance

Solid

Color

White to pink

Sequence

Cys-Ser-Cys-Ser-Ser-Leu-Met-Asp-Lys-Glu-Cys-Val-Tyr-Phe-Cys-His-Leu-Asp-Ile-Ile-Trp (Disulfide bridge: Cys1-Cys15, Cys3-Cys11)

Sequence Shortening

CSCSSLMDKECVYFCHLDIIW (Disulfide bridge: Cys1-Cys15, Cys3-Cys11)

Shipping

Room temperature in continental US; may vary elsewhere.

Storage

Sealed storage, away from moisture and light, under nitrogen

Powder	-80°C	2 years
	-20°C	1 year

*In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light, under nitrogen)

Solvent & Solubility

In Vitro:

DMSO : 50 mg/mL (20.07 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

H₂O : 0.99 mg/mL (0.40 mM; adjust pH to 2 with TFA)

Preparing
Stock Solutions

Concentration Solvent Mass	1 mg	5 mg	10 mg

1 mM	0.4013 mL	2.0065 mL	4.0130 mL
5 mM	0.0803 mL	0.4013 mL	0.8026 mL
10 mM	0.0401 mL	0.2007 mL	0.4013 mL

View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light, under nitrogen). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

Molarity Calculator
Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

This equation is commonly abbreviated as: C₁V₁ = C₂V₂

Concentration _(start)

Volume _(start)

Concentration _(final)

Volume _(final)

In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

Protocol 1

Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline
Solubility: ≥ 1.25 mg/mL (0.50 mM); Clear solution

This protocol yields a clear solution of ≥ 1.25 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (12.5 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.
Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.

In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Please enter your animal formula composition:

DMSO +

Tween-80 +

Saline

Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.

The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).

Calculation results:

Working solution concentration: mg/mL

Method for preparing stock solution: mg drug dissolved in μL DMSO (Stock solution concentration: mg/mL).

*In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light, under nitrogen)

The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only. If necessary, please contact MedChemExpress (MCE).

Method for preparing in vivo working solution for animal experiments: Take μL DMSO stock solution, add μL . μL , mix evenly, next add μL Tween 80, mix evenly, then add μL Saline.

If the continuous dosing period exceeds half a month, please choose this protocol carefully.

Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.

Purity & Documentation

Purity: 99.12%

Select Batch:

Data Sheet (276 KB) SDS (480 KB)

COA (248 KB) RP-HPLC (256 KB) LCMS (88 KB) Elemental Analysis Report (108 KB)

Handling Instructions (2659 KB)

References

[1]. Kowalczyk A, et al. The role of endothelin-1 and endothelin receptor antagonists in inflammatory response andsepsis. Arch Immunol Ther Exp (Warsz). 2015 Feb;63(1):41-52. [Content Brief]

Complete Stock Solution Preparation Table

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg

DMSO	1 mM	0.4013 mL	2.0065 mL	4.0130 mL	10.0325 mL
	5 mM	0.0803 mL	0.4013 mL	0.8026 mL	2.0065 mL
	10 mM	0.0401 mL	0.2007 mL	0.4013 mL	1.0033 mL
	15 mM	0.0268 mL	0.1338 mL	0.2675 mL	0.6688 mL
	20 mM	0.0201 mL	0.1003 mL	0.2007 mL	0.5016 mL

* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.