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  5. Hygromycin B

Hygromycin B  (Synonyms: Hygrovetine)

Cat. No.: HY-B0490 Purity: 99.93%
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Hygromycin B is an aminoglycoside antibiotic active against prokaryotic and eukaryotic cells.

For research use only. We do not sell to patients.

Hygromycin B Chemical Structure

Hygromycin B Chemical Structure

CAS No. : 31282-04-9

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Solid + Solvent (Highly Recommended)
10 mM * 1 mL in DMSO
ready for reconstitution
 USD 66 In-stock
Solution
10 mM * 1 mL in DMSO USD 66 In-stock
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1 g USD 108 In-stock
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Based on 21 publication(s) in Google Scholar

Other Forms of Hygromycin B:

Hygromycin B Related Antibodies

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  • Biological Activity

  • Protocol

  • Purity & Documentation

  • References

  • Customer Review

Description

Hygromycin B is an aminoglycoside antibiotic active against prokaryotic and eukaryotic cells.

IC50 & Target

Aminoglycoside

 

In Vitro

Hygromycin B, an aminocyclitol antibiotic that strongly inhibits both 70S and 80S ribosomes, is synthesized by Streptomyces hygroscopicus[1].
Hygromycin B at 0.38 mM concentration completely halts yeast cell growth in rich media, presumably by preventing protein synthesis by cytoplasmic ribosomes. Polypeptide synthesis in cell-free extracts from rabbit reticulocytes, wheat germ and yeast is strongly blocked by low concentrations ofhygromycin B. The antibiotic inhibits peptide chain elongation by yeast polysomes by preventing elongation factor EF-2-dependent translocation. The inhibition of translocation by hygromycin B might result from the stabilization of peptidyl-tRNA bound to the ribosomal acceptor site[2].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Hygromycin B Related Antibodies
In Vivo

Hygromycin B inhibits protein synthesis by blocking ribosomal translocation without causing significant misreading in vivo[3].
Constitutive expression of the bacterial hygR gene in transgenic mice in vivo confers resistance to hygromycin B[4].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
Molecular Weight

527.52

Formula

C20H37N3O13
CAS No.
Appearance

Solid

Color

White to light yellow

SMILES

O[C@H]([C@H]1O)C2(O[C@@]([C@@H](N)CO)([H])[C@@H]1O)O[C@@]([C@@H](O[C@@H]3CO)O[C@@]([C@H]([C@H](N)C4)O)([H])[C@@H]([C@H]4NC)O)([H])[C@@]([C@H]3O)([H])O2
Structure Classification
Initial Source

Escherichia coli
Shipping

Room temperature in continental US; may vary elsewhere.
Storage
Powder -20°C 3 years
4°C 2 years
In solvent -80°C 6 months
-20°C 1 month
Solvent & Solubility
In Vitro: 

DMSO : 100 mg/mL (189.57 mM; ultrasonic and warming and heat to 60°C; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

H2O : 50 mg/mL (94.78 mM; Need ultrasonic)

Preparing
Stock Solutions
Concentration Solvent Mass 1 mg 5 mg 10 mg
1 mM 1.8957 mL 9.4783 mL 18.9566 mL
5 mM 0.3791 mL 1.8957 mL 3.7913 mL
View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

  • Molarity Calculator

  • Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Mass
=
Concentration
×
Volume
×
Molecular Weight *

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start)

C1

×
Volume (start)

V1

=
Concentration (final)

C2

×
Volume (final)

V2

In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

  • Protocol 1

    Add each solvent one by one:  10% DMSO    40% PEG300    5% Tween-80    45% Saline

    Solubility: ≥ 5 mg/mL (9.48 mM); Clear solution

    This protocol yields a clear solution of ≥ 5 mg/mL (saturation unknown).

    Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (50.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.

    Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
  • Protocol 2

    Add each solvent one by one:  10% DMSO    90% (20% SBE-β-CD in Saline)

    Solubility: ≥ 5 mg/mL (9.48 mM); Clear solution

    This protocol yields a clear solution of ≥ 5 mg/mL (saturation unknown).

    Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (50.0 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.

    Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.

For the following dissolution methods, please prepare the working solution directly. It is recommended to prepare fresh solutions and use them promptly within a short period of time.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

  • Protocol 1

    Add each solvent one by one:  PBS

    Solubility: 100 mg/mL (189.57 mM); Clear solution; Need ultrasonic

In Vivo Dissolution Calculator
Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

g

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Calculation results:
Working solution concentration: mg/mL
This product has good water solubility, please refer to the measured solubility data in water/PBS/Saline for details.
The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only.If necessary, please contact MedChemExpress (MCE).
Purity & Documentation

Purity: 99.93%

SDS (480 KB)

COA (255 KB) HNMR (303 KB) RP-HPLC (223 KB)

Handling Instructions (2659 KB)
References
Animal Administration
[4]

Hygromycin B is dissolved in sterile water. The mice C57BL/6J-TgN(pPWL512hyg)1Ems carrying hygR are treated with a single dose of hygromycin B i.p. at doses that starts at 2.7 mg/kg and increases by 50% for each consecutive dose. Control wild-type C57BL/6J mice are treated with the same volume of sterile saline. Total volume injected is 0.5 mL. The health status and body weights of animals are monitored daily for 10 consecutive days[4].

MCE has not independently confirmed the accuracy of these methods. They are for reference only.
References

Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
H2O / DMSO 1 mM 1.8957 mL 9.4783 mL 18.9566 mL 47.3916 mL
5 mM 0.3791 mL 1.8957 mL 3.7913 mL 9.4783 mL
10 mM 0.1896 mL 0.9478 mL 1.8957 mL 4.7392 mL
15 mM 0.1264 mL 0.6319 mL 1.2638 mL 3.1594 mL
20 mM 0.0948 mL 0.4739 mL 0.9478 mL 2.3696 mL
25 mM 0.0758 mL 0.3791 mL 0.7583 mL 1.8957 mL
30 mM 0.0632 mL 0.3159 mL 0.6319 mL 1.5797 mL
40 mM 0.0474 mL 0.2370 mL 0.4739 mL 1.1848 mL
50 mM 0.0379 mL 0.1896 mL 0.3791 mL 0.9478 mL
60 mM 0.0316 mL 0.1580 mL 0.3159 mL 0.7899 mL
80 mM 0.0237 mL 0.1185 mL 0.2370 mL 0.5924 mL
DMSO 100 mM 0.0190 mL 0.0948 mL 0.1896 mL 0.4739 mL

* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

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Hygromycin B Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

Hygromycin B31282-04-9HygrovetineBacterialFungalAntibioticParasiteInhibitorinhibitorinhibit

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