INH6

Name: INH6
Brand: MedChemExpress
SKU: HY-100541
Rating: 4.5 (1 reviews)

Cat. No.: HY-100541 Purity: 99.90%: FAQs
Data Sheet SDS COA Handling Instructions

Molarity Calculator

INH6 is a potent Nek2/Hec1 inhibitor; inhibits the growth of HeLa cells with an IC₅₀ of 2.4 μM.

For research use only. We do not sell to patients.

INH6 Chemical Structure

CAS No. : 1001753-24-7

Size	Price	Stock	Quantity
Solid + Solvent (Highly Recommended)
10 mM * 1 mL in DMSO ready for reconstitution	USD 55	In-stock	Estimated Time of Arrival: December 31
Solution
10 mM * 1 mL in DMSO	USD 55	In-stock	Estimated Time of Arrival: December 31
Solid
5 mg	USD 32	In-stock	Estimated Time of Arrival: December 31
10 mg	USD 50	In-stock	Estimated Time of Arrival: December 31
25 mg	USD 110	In-stock	Estimated Time of Arrival: December 31
50 mg	USD 180	In-stock	Estimated Time of Arrival: December 31
100 mg	USD 340	In-stock	Estimated Time of Arrival: December 31
200 mg		Get quote
500 mg		Get quote

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Customer Review

Based on 1 publication(s) in Google Scholar

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Biological Activity
Protocol
Purity & Documentation
References
Customer Review

Description

INH6 is a potent Nek2/Hec1 inhibitor; inhibits the growth of HeLa cells with an IC₅₀ of 2.4 μM.

IC₅₀ & Target

NEK2

Cellular Effect

Cell Line	Type	Value	Description	References
HeLa	IC₅₀	2.4 μM Compound: 6	Cytotoxicity against human HeLa cells after 4 days by XTT assay Cytotoxicity against human HeLa cells after 4 days by XTT assay	[PMID: 19243176]
HeLa	IC₅₀	3.6 μM Compound: 2	Antiproliferative activity against human HeLa cells assessed as cell viability after 96 hrs by CellTiter96 assay Antiproliferative activity against human HeLa cells assessed as cell viability after 96 hrs by CellTiter96 assay	[PMID: 24773549]
K562	IC₅₀	2.5 μM Compound: 6	Cytotoxicity against human K562 cells after 4 days by XTT assay Cytotoxicity against human K562 cells after 4 days by XTT assay	[PMID: 19243176]
K562	IC₅₀	3.2 μM Compound: 2	Antiproliferative activity against human K562 cells assessed as cell viability after 96 hrs by CellTiter96 assay Antiproliferative activity against human K562 cells assessed as cell viability after 96 hrs by CellTiter96 assay	[PMID: 24773549]
MDA-MB-231	IC₅₀	1.7 μM Compound: 6	Cytotoxicity against human MDA-MB-231 cells after 4 days XTT assay Cytotoxicity against human MDA-MB-231 cells after 4 days XTT assay	[PMID: 19243176]
MDA-MB-231	IC₅₀	3.2 μM Compound: 2	Antiproliferative activity against human MDA-MB-231 cells assessed as cell viability after 96 hrs by CellTiter96 assay Antiproliferative activity against human MDA-MB-231 cells assessed as cell viability after 96 hrs by CellTiter96 assay	[PMID: 24773549]
MDA-MB-468	IC₅₀	2.1 μM Compound: 6	Cytotoxicity against human MDA-MB-468 cells after 4 days by XTT assay Cytotoxicity against human MDA-MB-468 cells after 4 days by XTT assay	[PMID: 19243176]
MDA-MB-468	IC₅₀	2.2 μM Compound: 2	Antiproliferative activity against human MDA-MB-468 cells assessed as cell viability after 96 hrs by CellTiter96 assay Antiproliferative activity against human MDA-MB-468 cells assessed as cell viability after 96 hrs by CellTiter96 assay	[PMID: 24773549]

In Vitro

Hec1 is an oncogene overly expressed in many human cancers. Small molecule INH (Inhibitor of Nek2/Hec1) targeting the Hec1 and its regulator, Nek2, in the mitotic pathway is identified to inactivate Hec1/Nek2 function mediated by protein degradation that subsequently leads to chromosome mis-segregation and cell death. INH6 treated cells exhibit increased mitotic population with multipolar spindle configurations. An increased rate of chromosome misalignment is detected upon treatment with INH6 of HeLa cells expressing the chromosome marker protein H2B-GFP. INH6 treated cells shows progressive morphological changes characteristic of dying cells (e.g., membrane bubbling), which is further confirmed by cell cycle profiling with FACS analysis. Approximately 20% of INH6 treated cells are apoptotic 72 hrs after treatment^[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Molecular Weight

322.42

Formula

C₁₉H₁₈N₂OS

CAS No.

1001753-24-7

Appearance

Solid

Color

White to off-white

SMILES

O=C(NC1=NC(C2=C(C)C=C(C)C=C2C)=CS1)C3=CC=CC=C3

Shipping

Room temperature in continental US; may vary elsewhere.

Storage

Powder	-20°C	3 years
	4°C	2 years
In solvent	-80°C	2 years
	-20°C	1 year

Solvent & Solubility

In Vitro:

DMSO : 50 mg/mL (155.08 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Preparing
Stock Solutions

Concentration Solvent Mass	1 mg	5 mg	10 mg

1 mM	3.1015 mL	15.5077 mL	31.0154 mL
5 mM	0.6203 mL	3.1015 mL	6.2031 mL
10 mM	0.3102 mL	1.5508 mL	3.1015 mL

View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 2 years; -20°C, 1 year. When stored at -80°C, please use it within 2 years. When stored at -20°C, please use it within 1 year.

Molarity Calculator
Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

This equation is commonly abbreviated as: C₁V₁ = C₂V₂

Concentration _(start)

Volume _(start)

Concentration _(final)

Volume _(final)

In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

Protocol 1

Add each solvent one by one: 10% DMSO 90% Corn Oil
Solubility: ≥ 2.5 mg/mL (7.75 mM); Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown). If the continuous dosing period exceeds half a month, please choose this protocol carefully.
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL Corn oil, and mix evenly.

In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Please enter your animal formula composition:

DMSO +

Tween-80 +

Saline

Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.

The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).

Calculation results:

Working solution concentration: mg/mL

Method for preparing stock solution: mg drug dissolved in μL DMSO (Stock solution concentration: mg/mL).

The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only. If necessary, please contact MedChemExpress (MCE).

Method for preparing in vivo working solution for animal experiments: Take μL DMSO stock solution, add μL . μL , mix evenly, next add μL Tween 80, mix evenly, then add μL Saline.

If the continuous dosing period exceeds half a month, please choose this protocol carefully.

Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.

Purity & Documentation

Purity: 99.90%

Select Batch:

Data Sheet (273 KB) SDS (251 KB)

COA (249 KB) LCMS (269 KB)

Handling Instructions (2659 KB)

References

[1]. Qiu XL, et al. Synthesis and biological evaluation of a series of novel inhibitor of Nek2/Hec1 analogues. J Med Chem. 2009 Mar 26;52(6):1757-67. [Content Brief]

Cell Assay
^[1]

Standard XTT assays with a four-day drug treatment procedure were performed to measure the dose-dependent cytotoxicity of INH analogs in cultured cells. Triplicate sets were measured and compiled for final data presentation. Cells were plated on 96-well dishes one day before the drug treatment, followed by drug treatment (2.5 μM INH6) on day 2 and XTT assay on day 5 after drug addition. The absorption at 595 nm was measured with a plate reader and converted to cell survival percentages in comparison to mock treated groups^[1].

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

References

[1]. Qiu XL, et al. Synthesis and biological evaluation of a series of novel inhibitor of Nek2/Hec1 analogues. J Med Chem. 2009 Mar 26;52(6):1757-67. [Content Brief]

Complete Stock Solution Preparation Table

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg

DMSO	1 mM	3.1015 mL	15.5077 mL	31.0154 mL	77.5386 mL
	5 mM	0.6203 mL	3.1015 mL	6.2031 mL	15.5077 mL
	10 mM	0.3102 mL	1.5508 mL	3.1015 mL	7.7539 mL
	15 mM	0.2068 mL	1.0338 mL	2.0677 mL	5.1692 mL
	20 mM	0.1551 mL	0.7754 mL	1.5508 mL	3.8769 mL
	25 mM	0.1241 mL	0.6203 mL	1.2406 mL	3.1015 mL
	30 mM	0.1034 mL	0.5169 mL	1.0338 mL	2.5846 mL
	40 mM	0.0775 mL	0.3877 mL	0.7754 mL	1.9385 mL
	50 mM	0.0620 mL	0.3102 mL	0.6203 mL	1.5508 mL
	60 mM	0.0517 mL	0.2585 mL	0.5169 mL	1.2923 mL
	80 mM	0.0388 mL	0.1938 mL	0.3877 mL	0.9692 mL
	100 mM	0.0310 mL	0.1551 mL	0.3102 mL	0.7754 mL

INH6 Related Classifications

Help & FAQs

Do most proteins show cross-species activity?
Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

INH61001753-24-7INH 6INH-6NEKsApoptosisNIMA–related KinasesNIMA (never in mitosis gene a)-related Expressed KinasesInhibitorinhibitorinhibit

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INH6

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INH6 Related Antibodies

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References

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INH6 Related Antibodies

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Complete Stock Solution Preparation Table

INH6 Related Classifications

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