2. Membrane Transporter/Ion Channel Anti-infection
  3. CFTR SARS-CoV
  4. IOWH-032

IOWH-032 is a a synthetic anti-secretory molecule, is a potent CFTR inhibitor with an IC50 value of 8 μM. IOWH-032 also is a anti-diarrheal agent.

For research use only. We do not sell to patients.

IOWH-032 Chemical Structure

IOWH-032 Chemical Structure

CAS No. : 1191252-49-9

Size Price Stock Quantity
Solid + Solvent (Highly Recommended)
10 mM * 1 mL in DMSO
ready for reconstitution
 USD 49 In-stock
Solution
10 mM * 1 mL in DMSO USD 49 In-stock
Solid
5 mg USD 41 In-stock
10 mg USD 66 In-stock
50 mg USD 176 In-stock
100 mg USD 275 In-stock
500 mg USD 825 In-stock
1 g   Get quote  
5 g   Get quote  

* Please select Quantity before adding items.

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Customer Review

Based on 4 publication(s) in Google Scholar

IOWH-032 Related Antibodies

Top Publications Citing Use of Products

  • Biological Activity

  • Purity & Documentation

  • References

  • Customer Review

Description

IOWH-032 is a a synthetic anti-secretory molecule, is a potent CFTR inhibitor with an IC50 value of 8 μM. IOWH-032 also is a anti-diarrheal agent[1][2].

In Vitro

IOWH-032 (10 μM; 0-72 h) increases the ACE-2 expression in SARS-CoV-2 infected CFBE41o- WT cells[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

IOWH-032 Related Antibodies

Western Blot Analysis[1]

Cell Line: CFBE41o- cells
Concentration: 10 µM
Incubation Time: 0, 24, 48, 72 h
Result: Increased the ACE-2 expression in SARS-CoV-2 infected CFBE41o- WT cells in a time-dependent manner.
Clinical Trial
Molecular Weight

545.18

Formula

C22H15Br2N3O4
CAS No.
Appearance

Solid

Color

White to off-white

SMILES

BrC1=C(O)C(Br)=CC(C2=NOC(C(NCC3=CC=C(OC4=CC=CC=C4)C=C3)=O)=N2)=C1
Shipping

Room temperature in continental US; may vary elsewhere.
Storage
Powder -20°C 3 years
4°C 2 years
In solvent -80°C 2 years
-20°C 1 year
Solvent & Solubility
In Vitro: 

DMSO : ≥ 100 mg/mL (183.43 mM; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

*"≥" means soluble, but saturation unknown.

Preparing
Stock Solutions
Concentration Solvent Mass 1 mg 5 mg 10 mg
1 mM 1.8343 mL 9.1713 mL 18.3426 mL
5 mM 0.3669 mL 1.8343 mL 3.6685 mL
View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 2 years; -20°C, 1 year. When stored at -80°C, please use it within 2 years. When stored at -20°C, please use it within 1 year.

  • Molarity Calculator

  • Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Mass
=
Concentration
×
Volume
×
Molecular Weight *

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start)

C1

×
Volume (start)

V1

=
Concentration (final)

C2

×
Volume (final)

V2

In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

  • Protocol 1

    Add each solvent one by one:  10% DMSO    40% PEG300    5% Tween-80    45% Saline

    Solubility: ≥ 2.5 mg/mL (4.59 mM); Clear solution

    This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).

    Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.

    Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
  • Protocol 2

    Add each solvent one by one:  10% DMSO    90% Corn Oil

    Solubility: ≥ 2.5 mg/mL (4.59 mM); Clear solution

    This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown). If the continuous dosing period exceeds half a month, please choose this protocol carefully.

    Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL Corn oil, and mix evenly.

In Vivo Dissolution Calculator
Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

g

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Please enter your animal formula composition:
%
DMSO +
% 
+
%
Tween-80 +
%
Saline
Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).
Calculation results:
Working solution concentration: mg/mL
Method for preparing stock solution: mg drug dissolved in μL  DMSO (Stock solution concentration: mg/mL).
The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only. If necessary, please contact MedChemExpress (MCE).
Method for preparing in vivo working solution for animal experiments: Take μL DMSO stock solution, add μL . μL , mix evenly, next add μL Tween 80, mix evenly, then add μL Saline.
 If the continuous dosing period exceeds half a month, please choose this protocol carefully.
Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.
Purity & Documentation

Purity: 99.63%

SDS (393 KB)

COA (248 KB) RP-HPLC (86 KB)

Handling Instructions (2659 KB)
References

Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 2 years; -20°C, 1 year. When stored at -80°C, please use it within 2 years. When stored at -20°C, please use it within 1 year.

Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
DMSO 1 mM 1.8343 mL 9.1713 mL 18.3426 mL 45.8564 mL
5 mM 0.3669 mL 1.8343 mL 3.6685 mL 9.1713 mL
10 mM 0.1834 mL 0.9171 mL 1.8343 mL 4.5856 mL
15 mM 0.1223 mL 0.6114 mL 1.2228 mL 3.0571 mL
20 mM 0.0917 mL 0.4586 mL 0.9171 mL 2.2928 mL
25 mM 0.0734 mL 0.3669 mL 0.7337 mL 1.8343 mL
30 mM 0.0611 mL 0.3057 mL 0.6114 mL 1.5285 mL
40 mM 0.0459 mL 0.2293 mL 0.4586 mL 1.1464 mL
50 mM 0.0367 mL 0.1834 mL 0.3669 mL 0.9171 mL
60 mM 0.0306 mL 0.1529 mL 0.3057 mL 0.7643 mL
80 mM 0.0229 mL 0.1146 mL 0.2293 mL 0.5732 mL
100 mM 0.0183 mL 0.0917 mL 0.1834 mL 0.4586 mL
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IOWH-032 Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

IOWH-0321191252-49-9IOWH032IOWH 032CFTRSARS-CoVCystic fibrosis transmembrane conductance regulatorSARS coronavirusSARS-CoV-2CFBE41o- WT cellsACE-2anti-diarrhealInhibitorinhibitorinhibit

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