1. Metabolic Enzyme/Protease
  2. Endogenous Metabolite
  3. Isobutyryl-L-carnitine

Isobutyryl-L-carnitine is a product of the acyl-CoA dehydrogenases.

At equivalent molar concentrations, both the salt and free forms of a compound exhibit comparable biological activity. Nevertheless, the salt form (Isobutyryl-L-carnitine chloride) usually boasts enhanced water solubility and stability.

For research use only. We do not sell to patients.

Isobutyryl-L-carnitine Chemical Structure

Isobutyryl-L-carnitine Chemical Structure

CAS No. : 25518-49-4

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Other Forms of Isobutyryl-L-carnitine:

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Description

Isobutyryl-L-carnitine is a product of the acyl-CoA dehydrogenases[1].

IC50 & Target

Human Endogenous Metabolite

 

Molecular Weight

231.29

Formula

C11H21NO4

CAS No.
SMILES

CC(C)C(O[C@H](CC([O-])=O)C[N+](C)(C)C)=O

Structure Classification
Initial Source
Shipping

Room temperature in continental US; may vary elsewhere.

Storage

Please store the product under the recommended conditions in the Certificate of Analysis.

Solvent & Solubility
In Vitro: 

DMSO : 250 mg/mL (1080.89 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

H2O : 125 mg/mL (540.45 mM; Need ultrasonic)

Preparing
Stock Solutions
Concentration Solvent Mass 1 mg 5 mg 10 mg
1 mM 4.3236 mL 21.6179 mL 43.2358 mL
5 mM 0.8647 mL 4.3236 mL 8.6472 mL
View the Complete Stock Solution Preparation Table

* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

  • Molarity Calculator

  • Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Mass
=
Concentration
×
Volume
×
Molecular Weight *

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start)

C1

×
Volume (start)

V1

=
Concentration (final)

C2

×
Volume (final)

V2

In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

  • Protocol 1

    Add each solvent one by one:  10% DMSO    40% PEG300    5% Tween-80    45% Saline

    Solubility: ≥ 2.08 mg/mL (8.99 mM); Clear solution

    This protocol yields a clear solution of ≥ 2.08 mg/mL (saturation unknown).

    Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (20.8 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.

    Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
  • Protocol 2

    Add each solvent one by one:  10% DMSO    90% (20% SBE-β-CD in Saline)

    Solubility: ≥ 2.08 mg/mL (8.99 mM); Clear solution

    This protocol yields a clear solution of ≥ 2.08 mg/mL (saturation unknown).

    Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (20.8 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.

    Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
In Vivo Dissolution Calculator
Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

g

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Calculation results:
Working solution concentration: mg/mL
This product has good water solubility, please refer to the measured solubility data in water/PBS/Saline for details.
The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only.If necessary, please contact MedChemExpress (MCE).
Purity & Documentation
References

Complete Stock Solution Preparation Table

Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
H2O / DMSO 1 mM 4.3236 mL 21.6179 mL 43.2358 mL 108.0894 mL
5 mM 0.8647 mL 4.3236 mL 8.6472 mL 21.6179 mL
10 mM 0.4324 mL 2.1618 mL 4.3236 mL 10.8089 mL
15 mM 0.2882 mL 1.4412 mL 2.8824 mL 7.2060 mL
20 mM 0.2162 mL 1.0809 mL 2.1618 mL 5.4045 mL
25 mM 0.1729 mL 0.8647 mL 1.7294 mL 4.3236 mL
30 mM 0.1441 mL 0.7206 mL 1.4412 mL 3.6030 mL
40 mM 0.1081 mL 0.5404 mL 1.0809 mL 2.7022 mL
50 mM 0.0865 mL 0.4324 mL 0.8647 mL 2.1618 mL
60 mM 0.0721 mL 0.3603 mL 0.7206 mL 1.8015 mL
80 mM 0.0540 mL 0.2702 mL 0.5404 mL 1.3511 mL
100 mM 0.0432 mL 0.2162 mL 0.4324 mL 1.0809 mL

* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

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Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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Product Name:
Isobutyryl-L-carnitine
Cat. No.:
HY-113165
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