2. Metabolic Enzyme/Protease Vitamin D Related/Nuclear Receptor Autophagy
  3. RAR/RXR Endogenous Metabolite Autophagy
  4. Isotretinoin

Isotretinoin  (Synonyms: 13-cis-Retinoic acid)

Cat. No.: HY-15127 Purity: 99.54%
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Isotretinoin (13-cis-Retinoic acid) is an orally active vitamin A derivative and is often be used for the research of severe acne. Isotretinoin also shows anticancer activity.

For research use only. We do not sell to patients.

Isotretinoin Chemical Structure

Isotretinoin Chemical Structure

CAS No. : 4759-48-2

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500 mg USD 126 In-stock
1 g USD 195 In-stock
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Based on 8 publication(s) in Google Scholar

Other Forms of Isotretinoin:

Isotretinoin Related Antibodies

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Description

Isotretinoin (13-cis-Retinoic acid) is an orally active vitamin A derivative and is often be used for the research of severe acne. Isotretinoin also shows anticancer activity[1][2].

IC50 & Target[2]

Human Endogenous Metabolite

 

Cellular Effect
Cell Line Type Value Description References
A549 IC50
35.8 μM
Compound: 3, 13-cis-RA
Cytotoxicity against human A549 cells after 24 hrs by MTT assay
Cytotoxicity against human A549 cells after 24 hrs by MTT assay
[PMID: 19231037]
BEL-7404 tumor cell line IC50
42.6 μM
Compound: 3, 13-cis-RA
Cytotoxicity against human Bel7404 cells after 24 hrs by MTT assay
Cytotoxicity against human Bel7404 cells after 24 hrs by MTT assay
[PMID: 19231037]
HL-60 ED50
100 nM
Compound: 2
Compound was evaluated for the retinoid-induced differentiation of the human myeloid leukemia cell line HL-60 using trans-retinoic acid as the standard.
Compound was evaluated for the retinoid-induced differentiation of the human myeloid leukemia cell line HL-60 using trans-retinoic acid as the standard.
[PMID: 1992144]
In Vitro

Isotretinoin (13-cis-Retinoic acid) may act as a pro-drug that is converted intracellularly to metabolites that are agonists for RAR and RXR nuclear receptors[1].
Isotretinoin is highly sensitive to air, heat, and light[3].
Isotretinoin (0-40 μM; 24-96 h) significantly inhibits HepG2 cell viability[4].
Isotretinoin (10 μM; 48 h) down-regulates c-MYC mRNA expression and this is partially due to P1 or P2 promoter activity[4].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Isotretinoin Related Antibodies

Cell Viability Assay[4]

Cell Line: HepG2
Concentration: 0.1, 0.5, 1, 5, 10, 20, and 40 μM
Incubation Time: 24, 48, 72, and 96 h
Result: Showed a significant reduction in cell viability (less than 30%) at 96 h for all doses.

RT-PCR[4]

Cell Line: HepG2
Concentration: 10 µM
Incubation Time: 48 h
Result: Significantly reduced the expression of c-MYC mRNA by ~80%.
In Vivo

Isotretinoin (13-cis-Retinoic acid) (1.3-481 μg/L; inhalation exposure; 45 min daily for 2 weeks) is an effective lung cancer chemopreventive agent in A/J Mice[2].
Isotretinoin accelerates alveolar repair after exodontia in rats[3].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model: Male A/J mice, lung carcinogenesis model[2]
Dosage: 1.3, 20.7, or 481 μg/L
Administration: Inhalation exposure, 45 min daily for 2 weeks
Result: Reduced the tumor multiplicity. Caused up-regulation of lung tissue nuclear retinoic acid receptors (RARs) relative to vehicle-exposed mice, RARα (3.9-fold vehicle), RARβ (3.3-fold), and RARγ (3.7-fold) at 20.7 μg/L.
Animal Model: Wistar rats, tooth extraction model[3]
Dosage: 7.5 mg/kg
Administration: Oral gavage, daily for 30 days
Result: Accelerated the process of alveolar repair, significantly decreased serum calcium levels.
Clinical Trial
Molecular Weight

300.44

Formula

C20H28O2
CAS No.
Appearance

Solid

Color

Yellow to orange

SMILES

CC1(C)C(/C=C/C(C)=C/C=C/C(C)=C\C(O)=O)=C(C)CCC1
Structure Classification
Initial Source
Shipping

Room temperature in continental US; may vary elsewhere.
Storage

-20°C, protect from light, stored under nitrogen

*The compound is unstable in solutions, freshly prepared is recommended.

Solvent & Solubility
In Vitro: 

DMSO : 100 mg/mL (332.85 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Preparing
Stock Solutions
Concentration Solvent Mass 1 mg 5 mg 10 mg
1 mM 3.3285 mL 16.6423 mL 33.2845 mL
5 mM 0.6657 mL 3.3285 mL 6.6569 mL
View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. The compound is unstable in solutions, freshly prepared is recommended.

  • Molarity Calculator

  • Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Mass
=
Concentration
×
Volume
×
Molecular Weight *

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start)

C1

×
Volume (start)

V1

=
Concentration (final)

C2

×
Volume (final)

V2

In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

  • Protocol 1

    Add each solvent one by one:  10% DMSO    40% PEG300    5% Tween-80    45% Saline

    Solubility: ≥ 2.5 mg/mL (8.32 mM); Clear solution

    This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).

    Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.

    Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
  • Protocol 2

    Add each solvent one by one:  10% DMSO    90% (20% SBE-β-CD in Saline)

    Solubility: 2.5 mg/mL (8.32 mM); Suspended solution; Need ultrasonic

    This protocol yields a suspended solution of 2.5 mg/mL. Suspended solution can be used for oral and intraperitoneal injection.

    Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.

    Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
In Vivo Dissolution Calculator
Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

g

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Please enter your animal formula composition:
%
DMSO +
% 
+
%
Tween-80 +
%
Saline
Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).
Calculation results:
Working solution concentration: mg/mL
Method for preparing stock solution: mg drug dissolved in μL  DMSO (Stock solution concentration: mg/mL).

*The compound is unstable in solutions, freshly prepared is recommended.

The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only. If necessary, please contact MedChemExpress (MCE).
Method for preparing in vivo working solution for animal experiments: Take μL DMSO stock solution, add μL . μL , mix evenly, next add μL Tween 80, mix evenly, then add μL Saline.
 If the continuous dosing period exceeds half a month, please choose this protocol carefully.
Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.
Purity & Documentation
References

Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. The compound is unstable in solutions, freshly prepared is recommended.

Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
DMSO 1 mM 3.3285 mL 16.6423 mL 33.2845 mL 83.2113 mL
5 mM 0.6657 mL 3.3285 mL 6.6569 mL 16.6423 mL
10 mM 0.3328 mL 1.6642 mL 3.3285 mL 8.3211 mL
15 mM 0.2219 mL 1.1095 mL 2.2190 mL 5.5474 mL
20 mM 0.1664 mL 0.8321 mL 1.6642 mL 4.1606 mL
25 mM 0.1331 mL 0.6657 mL 1.3314 mL 3.3285 mL
30 mM 0.1109 mL 0.5547 mL 1.1095 mL 2.7737 mL
40 mM 0.0832 mL 0.4161 mL 0.8321 mL 2.0803 mL
50 mM 0.0666 mL 0.3328 mL 0.6657 mL 1.6642 mL
60 mM 0.0555 mL 0.2774 mL 0.5547 mL 1.3869 mL
80 mM 0.0416 mL 0.2080 mL 0.4161 mL 1.0401 mL
100 mM 0.0333 mL 0.1664 mL 0.3328 mL 0.8321 mL
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Isotretinoin Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

Isotretinoin4759-48-213-cis-Retinoic acidRAR/RXREndogenous MetaboliteAutophagyRetinoic acid receptorsRetinoid X receptorsvitamin Asevere acneanticancerorally activeInhibitorinhibitorinhibit

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