2. Membrane Transporter/Ion Channel Neuronal Signaling
  3. GABA Receptor
  4. Jujuboside A

Jujuboside A is a glycoside extracted from Semen Ziziphi Spinosae, a Chinese herbal medicine used to treat insomnia and anxiety.

For research use only. We do not sell to patients.

Jujuboside A Chemical Structure

Jujuboside A Chemical Structure

CAS No. : 55466-04-1

Size Price Stock Quantity
Solid + Solvent (Highly Recommended)
10 mM * 1 mL in DMSO
ready for reconstitution
 USD 113 In-stock
Solution
10 mM * 1 mL in DMSO USD 113 In-stock
Solid
5 mg USD 50 In-stock
10 mg USD 85 In-stock
25 mg USD 180 In-stock
50 mg USD 310 In-stock
100 mg USD 520 In-stock
200 mg   Get quote  
500 mg   Get quote  

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Customer Review

Based on 3 publication(s) in Google Scholar

Jujuboside A Related Antibodies

Top Publications Citing Use of Products

  • Biological Activity

  • Protocol

  • Purity & Documentation

  • References

  • Customer Review

Description

Jujuboside A is a glycoside extracted from Semen Ziziphi Spinosae, a Chinese herbal medicine used to treat insomnia and anxiety.

IC50 & Target

GABA Receptor, mTOR, PI3K, Akt[1][2]
In Vitro

Jujuboside A at the low dose of 41 μM (about 0.05 g/L) induces significant increase of GABA(A) receptor α1, α5, β2 subunit mRNAs in both 24 and 72h treatments. Jujuboside A at the high dose of 82 μM (about 0.1 g/L) significantly increases GABA(A) receptor α1, α5 subunit mRNA levels and decreases β2 subunit mRNA level at 24h treatment, and decreases GABA(A) receptor subunit α1, β2 mRNAs expression at 72h treatment[1]. Jujuboside A pretreatment could reverse the reduction of cell viability and better the injury of H9C2 cells induced by ISO. Jujuboside A could accelerate the phosphorylation of PI3K, Akt, and mTOR. Jujuboside A could significantly decrease the ratio of microtubule-associated protein LC3-II/I in H9C2 cells[2].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Jujuboside A Related Antibodies
In Vivo

During daytime (9:00-15:00), jujubosides significantly increases the total sleep and rapid eye movement (REM) sleep without significant influence on non-REM (NREM) sleep. During nighttime (21:00-3:00), jujubosides significantly increases the total sleep and NREM sleep especially the light sleep while shows no significant effect on REM sleep and slow wave sleep (SWS)[3]. Intracerebroventricular treatment with Jujuboside A significantly mitigates learning and memory impairment in mice induced by Aβ1–42 as measured by the Y-maze, active avoidance and Morris water maze. Intracerebroventricular treatment with Jujuboside A reduces the level of Aβ1–42 in hippocampus, significantly inhibits the activities of acetylcholinesterase (AChE) and NO, and decreases the amount of the increased malondialdehyde (MDA) in the hippocampus and cerebral cortex of mice treated with intracerebroventricular injection of Aβ1–42[4].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
Molecular Weight

1207.35

Formula

C58H94O26
CAS No.
Appearance

Solid

Color

White to off-white

SMILES

C[C@]([C@]1([H])CC2)(CC[C@]3([H])[C@@]1(CC[C@H](O[C@@](OC[C@H](O)[C@@H]4O[C@@](O[C@H](CO[C@@H]([C@@H]([C@@H](O)[C@@H]5O)O)O[C@@H]5CO)[C@@H](O)[C@@H]6O)([H])[C@@H]6O[C@@](OC[C@@H](O)[C@@H]7O)([H])[C@@H]7O)([H])[C@@H]4O[C@@](O[C@@H](C)[C@H](O)[C@H]8O)([H])[C@@H]8O)C3(C)C)C)[C@@]9(CO%10)[C@@]2([H])[C@]([C@]%11(O)C)([H])[C@@]%10(O[C@@H](/C=C(C)/C)C%11)C9
Structure Classification
Initial Source
Shipping

Room temperature in continental US; may vary elsewhere.
Storage
Powder -20°C 3 years
4°C 2 years
In solvent -80°C 2 years
-20°C 1 year
Solvent & Solubility
In Vitro: 

DMSO : 100 mg/mL (82.83 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

H2O : 50 mg/mL (41.41 mM; Need ultrasonic)

Preparing
Stock Solutions
Concentration Solvent Mass 1 mg 5 mg 10 mg
1 mM 0.8283 mL 4.1413 mL 8.2826 mL
5 mM 0.1657 mL 0.8283 mL 1.6565 mL
View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 2 years; -20°C, 1 year. When stored at -80°C, please use it within 2 years. When stored at -20°C, please use it within 1 year.

* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

  • Molarity Calculator

  • Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Mass
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Concentration
×
Volume
×
Molecular Weight *

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start)

C1

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Volume (start)

V1

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Concentration (final)

C2

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Volume (final)

V2

In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

  • Protocol 1

    Add each solvent one by one:  10% DMSO    40% PEG300    5% Tween-80    45% Saline

    Solubility: ≥ 2.5 mg/mL (2.07 mM); Clear solution

    This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).

    Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.

    Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
  • Protocol 2

    Add each solvent one by one:  10% DMSO    90% (20% SBE-β-CD in Saline)

    Solubility: ≥ 2.5 mg/mL (2.07 mM); Clear solution

    This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).

    Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.

    Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.

For the following dissolution methods, please prepare the working solution directly. It is recommended to prepare fresh solutions and use them promptly within a short period of time.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

  • Protocol 1

    Add each solvent one by one:  PBS

    Solubility: 50 mg/mL (41.41 mM); Clear solution; Need ultrasonic

In Vivo Dissolution Calculator
Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

g

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Calculation results:
Working solution concentration: mg/mL
This product has good water solubility, please refer to the measured solubility data in water/PBS/Saline for details.
The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only.If necessary, please contact MedChemExpress (MCE).
Purity & Documentation

Purity: 99.88%

COA (272 KB) HNMR (390 KB) RP-HPLC (604 KB)

Handling Instructions (2659 KB)
References
Cell Assay
[1]

After 7 days in culture in vitro, cells are exposed continuously for the next 24 or 72 h to the culture medium with either Jujuboside A or diazepam, or neither of them. Vehicle is added for the control group; 10 μM diazepam is added for the diazepam group; Jujuboside A 82 μM (about 0.1 g/L) and 41 μM (about 0.05 g/L) are added for the JuA-H (high dose Jujuboside A) and the JuA-L (low dose Jujuboside A) groups respectively. Total RNA is isolated from cells for further analysis[1].

MCE has not independently confirmed the accuracy of these methods. They are for reference only.
Animal Administration
[1]

Mice: Cognitive impairment of mice is induced by ICV injection of Aβ1-42. Then mice are given intracerebroventricular (ICV) injection of Jujuboside A (0.02 and 0.2 mg/kg) for five consecutive days. Y-maze, active avoidance and Morris water maze tests are performed on mice[1].

MCE has not independently confirmed the accuracy of these methods. They are for reference only.
References

Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 2 years; -20°C, 1 year. When stored at -80°C, please use it within 2 years. When stored at -20°C, please use it within 1 year.

Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
H2O / DMSO 1 mM 0.8283 mL 4.1413 mL 8.2826 mL 20.7065 mL
5 mM 0.1657 mL 0.8283 mL 1.6565 mL 4.1413 mL
10 mM 0.0828 mL 0.4141 mL 0.8283 mL 2.0707 mL
15 mM 0.0552 mL 0.2761 mL 0.5522 mL 1.3804 mL
20 mM 0.0414 mL 0.2071 mL 0.4141 mL 1.0353 mL
25 mM 0.0331 mL 0.1657 mL 0.3313 mL 0.8283 mL
30 mM 0.0276 mL 0.1380 mL 0.2761 mL 0.6902 mL
40 mM 0.0207 mL 0.1035 mL 0.2071 mL 0.5177 mL
DMSO 50 mM 0.0166 mL 0.0828 mL 0.1657 mL 0.4141 mL
60 mM 0.0138 mL 0.0690 mL 0.1380 mL 0.3451 mL
80 mM 0.0104 mL 0.0518 mL 0.1035 mL 0.2588 mL

* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

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Jujuboside A Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

Jujuboside A55466-04-1GABA ReceptorGamma-aminobutyric acid Receptorγ-Aminobutyric acid ReceptorInhibitorinhibitorinhibit

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