L-Cysteine methyl ester hydrochloride

Name: L-Cysteine methyl ester hydrochloride
Brand: MedChemExpress
SKU: HY-B1038
Rating: 4.5 (1 reviews)

Cat. No.: HY-B1038 Purity: ≥98.0%: FAQs
Data Sheet SDS COA Handling Instructions

Molarity Calculator

L-Cysteine methyl ester hydrochloride is an antitussive, phlegmolytic agent used to relieve breathing difficulties caused by large amounts of phlegm. L-Cysteine methyl ester hydrochloride is a copper corrosion inhibitor used in various industrial studies.

For research use only. We do not sell to patients.

L-Cysteine methyl ester hydrochloride Chemical Structure

CAS No. : 18598-63-5

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Solid + Solvent (Highly Recommended)
10 mM * 1 mL in DMSO ready for reconstitution	USD 55	In-stock	Estimated Time of Arrival: December 31
Solution
10 mM * 1 mL in DMSO	USD 55	In-stock	Estimated Time of Arrival: December 31
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Customer Review

Based on 1 publication(s) in Google Scholar

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Biological Activity
Purity & Documentation
References
Customer Review

Description

In Vitro

L-Cysteine methyl ester hydrochloride (100 nM-100 μM) stimulates the secretory activity of trachea secretory cells, while high concentration stimulates the synthesis of mucinous glycoprotein in submucosal glands. It has an obvious viscosity-reducing effect on secretory cell mucous^[2].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

In Vivo

L-Cysteine methyl ester hydrochloride (500 μmol/kg, intravenous injection) can effectively overcome the harmful effects of morphine on respiration and gas exchange in Sprague Dawley rats without affecting the sedative or early anti-sensory effects of opioids^[3].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model:	Sprague Dawley rat model^[3]
Dosage:	500 μmol/kg
Administration:	10 mg/kg morphine i.v.,15 min later,500 μmol/kg L-CYSme and repeat after 15 min later.
Result:	Overcome the adverse effects of morphine on breathing, A-a gradient, and ABG chemistry in unanesthetized rats.

Molecular Weight

171.65

Formula

C₄H₁₀ClNO₂S

CAS No.

18598-63-5

Appearance

Solid

Color

White to off-white

SMILES

N[C@@H](CS)C(OC)=O.[H]Cl

Shipping

Room temperature in continental US; may vary elsewhere.

Storage

4°C, sealed storage, away from moisture

*In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)

Solvent & Solubility

In Vitro:

DMSO : 100 mg/mL (582.58 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Preparing
Stock Solutions

Concentration Solvent Mass	1 mg	5 mg	10 mg

1 mM	5.8258 mL	29.1290 mL	58.2581 mL
5 mM	1.1652 mL	5.8258 mL	11.6516 mL
10 mM	0.5826 mL	2.9129 mL	5.8258 mL

View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Molarity Calculator
Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

This equation is commonly abbreviated as: C₁V₁ = C₂V₂

Concentration _(start)

Volume _(start)

Concentration _(final)

Volume _(final)

In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

Protocol 1

Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline
Solubility: ≥ 2.5 mg/mL (14.56 mM); Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.
Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
Protocol 2

Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in Saline)
Solubility: ≥ 2.5 mg/mL (14.56 mM); Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.
Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
Protocol 3

Add each solvent one by one: 10% DMSO 90% Corn Oil
Solubility: ≥ 2.5 mg/mL (14.56 mM); Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown). If the continuous dosing period exceeds half a month, please choose this protocol carefully.
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL Corn oil, and mix evenly.

In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Please enter your animal formula composition:

DMSO +

Tween-80 +

Saline

Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.

The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).

Calculation results:

Working solution concentration: mg/mL

Method for preparing stock solution: mg drug dissolved in μL DMSO (Stock solution concentration: mg/mL).

*In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)

The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only. If necessary, please contact MedChemExpress (MCE).

Method for preparing in vivo working solution for animal experiments: Take μL DMSO stock solution, add μL . μL , mix evenly, next add μL Tween 80, mix evenly, then add μL Saline.

If the continuous dosing period exceeds half a month, please choose this protocol carefully.

Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.

Purity & Documentation

Purity: ≥98.0%

Select Batch:

Data Sheet (274 KB) SDS (393 KB)

COA (248 KB) HNMR (221 KB)

Handling Instructions (2659 KB)

References

[1]. Zarrouk A, et al. Temperature effect, activation energies and thermodynamic adsorption studies of L-cysteine methyl ester hydrochloride as copper corrosion inhibitor in nitric acid 2M. Int. J. Electrochem. Sci, 2011, 6(12): 6261-6274..

[2]. Yanaura S, et al. Behavior of mucus glycoproteins of tracheal secretory cells following L-cysteine methyl ester treatment. J Pharmacobiodyn. 1982 Aug;5(8):603-10. [Content Brief]

[3]. Getsy PM, et al. L-cysteine methyl ester overcomes the deleterious effects of morphine on ventilatory parameters and arterial blood-gas chemistry in unanesthetized rats. Front Pharmacol. 2022 Sep 28;13:968378. [Content Brief]

Complete Stock Solution Preparation Table

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg

DMSO	1 mM	5.8258 mL	29.1290 mL	58.2581 mL	145.6452 mL
	5 mM	1.1652 mL	5.8258 mL	11.6516 mL	29.1290 mL
	10 mM	0.5826 mL	2.9129 mL	5.8258 mL	14.5645 mL
	15 mM	0.3884 mL	1.9419 mL	3.8839 mL	9.7097 mL
	20 mM	0.2913 mL	1.4565 mL	2.9129 mL	7.2823 mL
	25 mM	0.2330 mL	1.1652 mL	2.3303 mL	5.8258 mL
	30 mM	0.1942 mL	0.9710 mL	1.9419 mL	4.8548 mL
	40 mM	0.1456 mL	0.7282 mL	1.4565 mL	3.6411 mL
	50 mM	0.1165 mL	0.5826 mL	1.1652 mL	2.9129 mL
	60 mM	0.0971 mL	0.4855 mL	0.9710 mL	2.4274 mL
	80 mM	0.0728 mL	0.3641 mL	0.7282 mL	1.8206 mL
	100 mM	0.0583 mL	0.2913 mL	0.5826 mL	1.4565 mL