2. Cell Cycle/DNA Damage
  3. DNA Stain
  4. LDS-751

Lds-751 is a nucleic acid stain that mainly detects DNA. Lds-751 is a nucleic acid stain that mainly detects DNA. Lds-751 has a high affinity for DNA and fluorescence is enhanced after binding, but the maximum emission wavelength is 670nm. Lds-751 and Thiazole orange can be used for the differentiation of red blood cells, platelets, reticulocytes, and nucleated cells and can be stimulated at 488nm. Studies have shown that LDS-751 binds almost exclusively to mitochondria when incubated with nucleated living cells. After nucleated Acridine Orange (HY-101879) staining and LDS-751 treatment of cells, confocal microscopy revealed almost no co-location of the cells. Staining with Rhodamine 123 (HY-D0816), a dye known to bind polarized mitochondria, was almost identical to the pattern observed with LDS-751.

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LDS-751 Chemical Structure

LDS-751 Chemical Structure

CAS No. : 181885-68-7

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5 mg USD 115 In-stock
10 mg USD 205 In-stock
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Based on 1 publication(s) in Google Scholar

LDS-751 Related Antibodies

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  • Protocol

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Description

Lds-751 is a nucleic acid stain that mainly detects DNA. Lds-751 is a nucleic acid stain that mainly detects DNA. Lds-751 has a high affinity for DNA and fluorescence is enhanced after binding, but the maximum emission wavelength is 670nm. Lds-751 and Thiazole orange can be used for the differentiation of red blood cells, platelets, reticulocytes, and nucleated cells and can be stimulated at 488nm. Studies have shown that LDS-751 binds almost exclusively to mitochondria when incubated with nucleated living cells. After nucleated Acridine Orange (HY-101879) staining and LDS-751 treatment of cells, confocal microscopy revealed almost no co-location of the cells. Staining with Rhodamine 123 (HY-D0816), a dye known to bind polarized mitochondria, was almost identical to the pattern observed with LDS-751[1][2][3].

In Vitro

General Protocol
Preparation of Lds-751 working solution
1.1 Preparation of the stock solution:
Dissolve 10 mg of Lds-751 in 1 mL of ddH2O.
Note: It is recommended to store the stock solution at 4°C-20°C away from light and avoid repetitive freeze-thaw cycles.
1.2 Preparation of Lds-751 working solution:
Dilute the stock solution in serum-free cell culture medium or PBS to obtain final concentration 10μg/mL Lds-751 working solution.
Note: Please adjust the concentration of Lds-751 working solution according to the actual situation.

Cell staining
2.1 Cell preparation
For suspension cells: Centrifuge at 1000 g at 4°C for 3-5 minutes and then discard the supernatant. Wash twice with PBS, 5 minutes each time.
For adherent cells: Discard the cell culture medium, and add trypsin to dissociate cells to make a single-cell suspension. Centrifuge at 1000 g at 4°C for 3-5 minutes and then discard the supernatant. Wash twice with PBS, 5 minutes each time.
2.2 Fix cells with 3.7% formaldehyde for 10 minutes, discard the fixative and rinsed with PBS for three times, 5 minutes each time.
2.3 Permeate cells with 0.2% Triton X-100 for 5 minutes, wash three times with PBS, 5 minutes each time.
2.4 Add 1 mL of Lds-751 working solution, and then incubate at room temperature for 1-5 minutes.
2.5 Centrifuge at 400 g at 4°C for 3-4 minutes and then discard the supernatant.
2.6 Wash twice with PBS, 5 minutes each time.
2.7 Resuspend cells with serum-free cell culture medium or PBS, and then detect by fluorescence microscope or flow cytometer.

Storage
-20°C, 1 year. Protect from light

Precautions
1. It is recommended to store the stock solution at -20℃ or -80℃ away from light and avoid repetitive freeze-thaw cycles.
2. Detect the fluorescence as soon as possible to avoid fluorescence quenching.
3. Lds-751 is sensitive to light, please operate away from light.
4. It is recommended to use AntiFade Mounting Medium (MCE Cat. No.: HY-K1042) to slow the fluorescence quenching.
5. Please adjust the concentration of Lds-751 working solution according to the actual situation.
6. This product is for R&D use only, not for drug, household, or other uses.
7. For your safety and health, please wear a lab coat and disposable gloves to operate.

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

LDS-751 Related Antibodies
Molecular Weight

471.98

Formula

C25H30ClN3O4
CAS No.
Appearance

Solid

Color

Green to black

Emission (Em)

515
Excitation (Ex)

488
SMILES

CC[N+]1=C2C=CC(N(C)C)=CC2=CC=C1/C=C/C=C/C3=CC=C(N(C)C)C=C3.O=Cl(=O)([O-])=O
Shipping

Room temperature in continental US; may vary elsewhere.
Storage

-20°C, sealed storage, away from moisture and light

*The compound is unstable in solutions, freshly prepared is recommended.

Solvent & Solubility
In Vitro: 

DMSO : 83.33 mg/mL (176.55 mM; ultrasonic and warming and heat to 60°C; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Preparing
Stock Solutions
Concentration Solvent Mass 1 mg 5 mg 10 mg
1 mM 2.1187 mL 10.5937 mL 21.1873 mL
5 mM 0.4237 mL 2.1187 mL 4.2375 mL
View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. The compound is unstable in solutions, freshly prepared is recommended.

  • Molarity Calculator

  • Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Mass
=
Concentration
×
Volume
×
Molecular Weight *

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start)

C1

×
Volume (start)

V1

=
Concentration (final)

C2

×
Volume (final)

V2

In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

  • Protocol 1

    Add each solvent one by one:  10% DMSO    40% PEG300    5% Tween-80    45% Saline

    Solubility: ≥ 2.08 mg/mL (4.41 mM); Clear solution

    This protocol yields a clear solution of ≥ 2.08 mg/mL (saturation unknown).

    Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (20.8 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.

    Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
  • Protocol 2

    Add each solvent one by one:  10% DMSO    90% (20% SBE-β-CD in Saline)

    Solubility: ≥ 2.08 mg/mL (4.41 mM); Clear solution

    This protocol yields a clear solution of ≥ 2.08 mg/mL (saturation unknown).

    Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (20.8 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.

    Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
In Vivo Dissolution Calculator
Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

g

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Please enter your animal formula composition:
%
DMSO +
% 
+
%
Tween-80 +
%
Saline
Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).
Calculation results:
Working solution concentration: mg/mL
Method for preparing stock solution: mg drug dissolved in μL  DMSO (Stock solution concentration: mg/mL).

*The compound is unstable in solutions, freshly prepared is recommended.

The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only. If necessary, please contact MedChemExpress (MCE).
Method for preparing in vivo working solution for animal experiments: Take μL DMSO stock solution, add μL . μL , mix evenly, next add μL Tween 80, mix evenly, then add μL Saline.
 If the continuous dosing period exceeds half a month, please choose this protocol carefully.
Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.
Purity & Documentation

Purity: 99.30%

COA (246 KB) HNMR (223 KB) RP-HPLC (252 KB) MS (68 KB)

Handling Instructions (2659 KB)
References
Cell Assay

Blood samples (1 mL) are obtained using a sterile Butterfly-21 needle and plastic syringe from the antecubital vein of normal healthy volunteers who have given their informed consent. They are immediately transferred to plastic tubes containing 17.3 mg of phenylmethylsulphonyl fluoride (PMSF) and 1 mL of LDS-751 at room temperature. Aliquots (25 μL) are incubated for 5 min with between 2 μL and 5 μL of undiluted monoclonal antibodies, then diluted with 0.5 mL of 1% BSA in Hepesbuffered Hanks' balanced salts solution (HHBSS) and examined by flow cytometry[2].

MCE has not independently confirmed the accuracy of these methods. They are for reference only.
References

Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. The compound is unstable in solutions, freshly prepared is recommended.

Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
DMSO 1 mM 2.1187 mL 10.5937 mL 21.1873 mL 52.9683 mL
5 mM 0.4237 mL 2.1187 mL 4.2375 mL 10.5937 mL
10 mM 0.2119 mL 1.0594 mL 2.1187 mL 5.2968 mL
15 mM 0.1412 mL 0.7062 mL 1.4125 mL 3.5312 mL
20 mM 0.1059 mL 0.5297 mL 1.0594 mL 2.6484 mL
25 mM 0.0847 mL 0.4237 mL 0.8475 mL 2.1187 mL
30 mM 0.0706 mL 0.3531 mL 0.7062 mL 1.7656 mL
40 mM 0.0530 mL 0.2648 mL 0.5297 mL 1.3242 mL
50 mM 0.0424 mL 0.2119 mL 0.4237 mL 1.0594 mL
60 mM 0.0353 mL 0.1766 mL 0.3531 mL 0.8828 mL
80 mM 0.0265 mL 0.1324 mL 0.2648 mL 0.6621 mL
100 mM 0.0212 mL 0.1059 mL 0.2119 mL 0.5297 mL
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LDS-751 Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

LDS-751181885-68-7LDS751LDS 751DNA StainnucleicacidstainDNAfluorescenceInhibitorinhibitorinhibit

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