Lucanthone

Name: Lucanthone
Brand: MedChemExpress
SKU: HY-B2098
Rating: 4.5 (1 reviews)

Cat. No.: HY-B2098 Purity: 99.75%: FAQs
Data Sheet SDS COA Handling Instructions

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Lucanthone is an endonuclease inhibitor of Apurinic endonuclease-1 (APE-1).

For research use only. We do not sell to patients.

Lucanthone Chemical Structure

CAS No. : 479-50-5

Size	Price	Stock	Quantity

Free Sample (0.1 - 0.5 mg)		Apply Now
Solid + Solvent (Highly Recommended)
10 mM * 1 mL in DMSO ready for reconstitution	USD 176	In-stock	Estimated Time of Arrival: December 31
Solution
10 mM * 1 mL in DMSO	USD 176	In-stock	Estimated Time of Arrival: December 31
Solid
5 mg	USD 160	In-stock	Estimated Time of Arrival: December 31
10 mg	USD 255	In-stock	Estimated Time of Arrival: December 31
25 mg	USD 510	In-stock	Estimated Time of Arrival: December 31
50 mg	USD 815	In-stock	Estimated Time of Arrival: December 31
100 mg	USD 1300	In-stock	Estimated Time of Arrival: December 31
200 mg		Get quote
500 mg		Get quote

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Customer Review

Based on 1 publication(s) in Google Scholar

Other Forms of Lucanthone:

Lucanthone-d4 hydrochloride Get quote
Lucanthone hydrochloride Get quote

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Biological Activity
Protocol
Purity & Documentation
References
Customer Review

Description

Lucanthone is an endonuclease inhibitor of Apurinic endonuclease-1 (APE-1).

IC₅₀ & Target

APE-1^[1]

Cellular Effect

Cell Line	Type	Value	Description	References
BT-20	IC₅₀	5.7 μM Compound: Lucanthone	Cytotoxicity against human BT20 cells after 72 hrs by MTT assay Cytotoxicity against human BT20 cells after 72 hrs by MTT assay	[PMID: 25699157]
BT-474	IC₅₀	5.7 μM Compound: Lucanthone	Cytotoxicity against human BT474 cells after 72 hrs by MTT assay Cytotoxicity against human BT474 cells after 72 hrs by MTT assay	[PMID: 25699157]
HCC1937	IC₅₀	5.7 μM Compound: Lucanthone	Cytotoxicity against human HCC1937 cells after 72 hrs by MTT assay Cytotoxicity against human HCC1937 cells after 72 hrs by MTT assay	[PMID: 25699157]
HCC1954	IC₅₀	5.7 μM Compound: Lucanthone	Cytotoxicity against human HCC1954 cells after 72 hrs by MTT assay Cytotoxicity against human HCC1954 cells after 72 hrs by MTT assay	[PMID: 25699157]
MDA-MB-231	IC₅₀	5.7 μM Compound: Lucanthone	Cytotoxicity against human MDA-MB-231 cells after 72 hrs by MTT assay Cytotoxicity against human MDA-MB-231 cells after 72 hrs by MTT assay	[PMID: 25699157]
MDA-MB-435	IC₅₀	5.7 μM Compound: Lucanthone	Cytotoxicity against human MDA-MB-435 cells after 72 hrs by MTT assay Cytotoxicity against human MDA-MB-435 cells after 72 hrs by MTT assay	[PMID: 25699157]
SK-BR-3	IC₅₀	5.7 μM Compound: Lucanthone	Cytotoxicity against human SKBR3 cells after 72 hrs by MTT assay Cytotoxicity against human SKBR3 cells after 72 hrs by MTT assay	[PMID: 25699157]

In Vitro

Lucanthone is a novel inhibitor of autophagy that induces cathepsin D-mediated apoptosis. To investigate the anticancer activity of Lucanthone, cell viability is measured by MTT assay. Lucanthone reduces cell viability to a similar extent in a panel of seven breast cancer cell lines. In addition, a direct comparison reveals that Lucanthone is significantly more potent than Chloroquine (CQ) at reducing breast cancer cell viability with a mean IC₅₀ of 7.2 μM versus 66 μM for CQ. Measurement of cell viability in two representative cell lines (MDA-MB-231 and BT-20) by ATPlite assay and trypan blue exclusion reveals comparable results^[2].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Clinical Trial

Molecular Weight

340.48

Formula

C₂₀H₂₄N₂OS

CAS No.

479-50-5

Appearance

Solid

Color

Off-white to orange

SMILES

O=C1C2=C(SC3=C1C=CC=C3)C(C)=CC=C2NCCN(CC)CC

Shipping

Room temperature in continental US; may vary elsewhere.

Storage

Powder	-20°C	3 years
	4°C	2 years
In solvent	-80°C	2 years
	-20°C	1 year

Solvent & Solubility

In Vitro:

DMSO : 25 mg/mL (73.43 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Preparing
Stock Solutions

Concentration Solvent Mass	1 mg	5 mg	10 mg

1 mM	2.9370 mL	14.6852 mL	29.3703 mL
5 mM	0.5874 mL	2.9370 mL	5.8741 mL
10 mM	0.2937 mL	1.4685 mL	2.9370 mL

View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 2 years; -20°C, 1 year. When stored at -80°C, please use it within 2 years. When stored at -20°C, please use it within 1 year.

Molarity Calculator
Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

This equation is commonly abbreviated as: C₁V₁ = C₂V₂

Concentration _(start)

Volume _(start)

Concentration _(final)

Volume _(final)

In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

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Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Calculation results:

Working solution concentration: mg/mL

Purity & Documentation

Purity: 99.75%

Select Batch:

Data Sheet (274 KB) SDS (393 KB)

COA (250 KB) LCMS (265 KB)

Handling Instructions (2659 KB)

References

[1]. Chowdhury SM, et al. Graphene nanoribbons as a drug delivery agent for lucanthone mediated therapy of glioblastoma multiforme. Nanomedicine. 2015 Jan;11(1):109-18. [Content Brief]

[2]. Carew JS, et al. Lucanthone is a novel inhibitor of autophagy that induces cathepsin D-mediated apoptosis. J Biol Chem. 2011 Feb 25;286(8):6602-13. [Content Brief]

Cell Assay
^[2]

Cell viability is assessed by MTT assay. Cells are seeded into 96-well microculture plates at 10,000 cells per well and allowed to attach for 24 h. Cells are then treated with Lucanthone (0, 0.5, 1, 5, 10, 20 and 40 μM), Chloroquine, Vorinostat, or combinations for 72 h. Following drug treatment, MTT is added and cell viability is quantified using a BioTek microplate reader. Effects on cell viability are also determined by measuring ATP levels using the ATPlite assay system and by trypan blue exclusion. Pro-apoptotic effects following in vitro drug exposure are quantified by propidium iodide (PI) staining and fluorescence-activated cell sorting (FACS) analysis of sub-G₀/G₁ DNA content^[2].

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

References

[1]. Chowdhury SM, et al. Graphene nanoribbons as a drug delivery agent for lucanthone mediated therapy of glioblastoma multiforme. Nanomedicine. 2015 Jan;11(1):109-18. [Content Brief]

[2]. Carew JS, et al. Lucanthone is a novel inhibitor of autophagy that induces cathepsin D-mediated apoptosis. J Biol Chem. 2011 Feb 25;286(8):6602-13. [Content Brief]

Complete Stock Solution Preparation Table

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg

DMSO	1 mM	2.9370 mL	14.6851 mL	29.3703 mL	73.4257 mL
	5 mM	0.5874 mL	2.9370 mL	5.8741 mL	14.6851 mL
	10 mM	0.2937 mL	1.4685 mL	2.9370 mL	7.3426 mL
	15 mM	0.1958 mL	0.9790 mL	1.9580 mL	4.8950 mL
	20 mM	0.1469 mL	0.7343 mL	1.4685 mL	3.6713 mL
	25 mM	0.1175 mL	0.5874 mL	1.1748 mL	2.9370 mL
	30 mM	0.0979 mL	0.4895 mL	0.9790 mL	2.4475 mL
	40 mM	0.0734 mL	0.3671 mL	0.7343 mL	1.8356 mL
	50 mM	0.0587 mL	0.2937 mL	0.5874 mL	1.4685 mL
	60 mM	0.0490 mL	0.2448 mL	0.4895 mL	1.2238 mL

Lucanthone Related Classifications

Help & FAQs

Do most proteins show cross-species activity?
Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

Lucanthone479-50-5AutophagyParasiteInhibitorinhibitorinhibit

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Lucanthone

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Other Forms of Lucanthone:

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Complete Stock Solution Preparation Table

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