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  4. Lucanthone

Lucanthone is an endonuclease inhibitor of Apurinic endonuclease-1 (APE-1).

For research use only. We do not sell to patients.

Lucanthone Chemical Structure

Lucanthone Chemical Structure

CAS No. : 479-50-5

Size Price Stock Quantity
Solid + Solvent (Highly Recommended)
10 mM * 1 mL in DMSO
ready for reconstitution
 USD 176 In-stock
Solution
10 mM * 1 mL in DMSO USD 176 In-stock
Solid
1 mg USD 65 In-stock
5 mg USD 160 In-stock
10 mg USD 255 In-stock
25 mg USD 510 In-stock
50 mg USD 815 In-stock
100 mg USD 1300 In-stock
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Other Forms of Lucanthone:

Lucanthone Related Antibodies

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  • Biological Activity

  • Protocol

  • Purity & Documentation

  • References

  • Customer Review

Description

Lucanthone is an endonuclease inhibitor of Apurinic endonuclease-1 (APE-1).

IC50 & Target

APE-1[1]
In Vitro

Lucanthone is a novel inhibitor of autophagy that induces cathepsin D-mediated apoptosis. To investigate the anticancer activity of Lucanthone, cell viability is measured by MTT assay. Lucanthone reduces cell viability to a similar extent in a panel of seven breast cancer cell lines. In addition, a direct comparison reveals that Lucanthone is significantly more potent than Chloroquine (CQ) at reducing breast cancer cell viability with a mean IC50 of 7.2 μM versus 66 μM for CQ. Measurement of cell viability in two representative cell lines (MDA-MB-231 and BT-20) by ATPlite assay and trypan blue exclusion reveals comparable results[2].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Lucanthone Related Antibodies
Clinical Trial
Molecular Weight

340.48

Formula

C20H24N2OS
CAS No.
Appearance

Solid

Color

Off-white to orange

SMILES

O=C1C2=C(SC3=C1C=CC=C3)C(C)=CC=C2NCCN(CC)CC
Shipping

Room temperature in continental US; may vary elsewhere.
Storage
Powder -20°C 3 years
4°C 2 years
In solvent -80°C 2 years
-20°C 1 year
Solvent & Solubility
In Vitro: 

DMSO : 25 mg/mL (73.43 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Preparing
Stock Solutions
Concentration Solvent Mass 1 mg 5 mg 10 mg
1 mM 2.9370 mL 14.6852 mL 29.3703 mL
5 mM 0.5874 mL 2.9370 mL 5.8741 mL
View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 2 years; -20°C, 1 year. When stored at -80°C, please use it within 2 years. When stored at -20°C, please use it within 1 year.

  • Molarity Calculator

  • Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

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This equation is commonly abbreviated as: C1V1 = C2V2

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In Vivo Dissolution Calculator
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Calculation results:
Working solution concentration: mg/mL
Purity & Documentation
References
Cell Assay
[2]

Cell viability is assessed by MTT assay. Cells are seeded into 96-well microculture plates at 10,000 cells per well and allowed to attach for 24 h. Cells are then treated with Lucanthone (0, 0.5, 1, 5, 10, 20 and 40 μM), Chloroquine, Vorinostat, or combinations for 72 h. Following drug treatment, MTT is added and cell viability is quantified using a BioTek microplate reader. Effects on cell viability are also determined by measuring ATP levels using the ATPlite assay system and by trypan blue exclusion. Pro-apoptotic effects following in vitro drug exposure are quantified by propidium iodide (PI) staining and fluorescence-activated cell sorting (FACS) analysis of sub-G0/G1 DNA content[2].

MCE has not independently confirmed the accuracy of these methods. They are for reference only.
References

Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 2 years; -20°C, 1 year. When stored at -80°C, please use it within 2 years. When stored at -20°C, please use it within 1 year.

Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
DMSO 1 mM 2.9370 mL 14.6851 mL 29.3703 mL 73.4257 mL
5 mM 0.5874 mL 2.9370 mL 5.8741 mL 14.6851 mL
10 mM 0.2937 mL 1.4685 mL 2.9370 mL 7.3426 mL
15 mM 0.1958 mL 0.9790 mL 1.9580 mL 4.8950 mL
20 mM 0.1469 mL 0.7343 mL 1.4685 mL 3.6713 mL
25 mM 0.1175 mL 0.5874 mL 1.1748 mL 2.9370 mL
30 mM 0.0979 mL 0.4895 mL 0.9790 mL 2.4475 mL
40 mM 0.0734 mL 0.3671 mL 0.7343 mL 1.8356 mL
50 mM 0.0587 mL 0.2937 mL 0.5874 mL 1.4685 mL
60 mM 0.0490 mL 0.2448 mL 0.4895 mL 1.2238 mL
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Lucanthone Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

Lucanthone479-50-5AutophagyParasiteInhibitorinhibitorinhibit

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