2. Cell Cycle/DNA Damage Epigenetics
  3. Sirtuin
  4. MC3482

MC3482 is a specific sirtuin5 (SIRT5) inhibitor.

For research use only. We do not sell to patients.

MC3482 Chemical Structure

MC3482 Chemical Structure

CAS No. : 2922280-86-0

Size Price Stock Quantity
5 mg USD 140 In-stock
10 mg USD 230 In-stock
25 mg USD 360 In-stock
50 mg USD 570 In-stock
100 mg USD 920 In-stock
200 mg   Get quote  
500 mg   Get quote  

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Customer Review

Based on 5 publication(s) in Google Scholar

MC3482 Related Antibodies

Top Publications Citing Use of Products

View All Sirtuin Isoform Specific Products:

View All Isoforms
Sirtuin SIRT1 SIRT2 SIRT3 SIRT6 SIRT5 SIRT7

  • Biological Activity

  • Protocol

  • Purity & Documentation

  • References

  • Customer Review

Description

MC3482 is a specific sirtuin5 (SIRT5) inhibitor.

IC50 & Target

SIRT5[1]
In Vitro

MC3482 inhibits sirtuin5. 50 μM MC3482 inhibits SIRT5 desuccinylating activity without affecting SIRT5 intracellular expression levels. Autophagy and mitophagy increase in SIRT5-silenced cells and in WT cells treated with MC3482 and decrease in SIRT5-overexpressing cells[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

MC3482 Related Antibodies
Molecular Weight

618.68

Formula

C33H38N4O8
CAS No.
Appearance

Solid

Color

White to off-white

SMILES

O=C(N[C@H](C(NC1=CC=CC=C1)=O)CCCCNC(CC[C@@H](C(O)=O)NC(OCC2=CC=CC=C2)=O)=O)OCC3=CC=CC=C3
Shipping

Room temperature in continental US; may vary elsewhere.
Storage
Powder -20°C 3 years
4°C 2 years
In solvent -80°C 2 years
-20°C 1 year
Solvent & Solubility
In Vitro: 

DMSO : 125 mg/mL (202.04 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Preparing
Stock Solutions
Concentration Solvent Mass 1 mg 5 mg 10 mg
1 mM 1.6163 mL 8.0817 mL 16.1634 mL
5 mM 0.3233 mL 1.6163 mL 3.2327 mL
View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 2 years; -20°C, 1 year. When stored at -80°C, please use it within 2 years. When stored at -20°C, please use it within 1 year.

  • Molarity Calculator

  • Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Mass
=
Concentration
×
Volume
×
Molecular Weight *

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start)

C1

×
Volume (start)

V1

=
Concentration (final)

C2

×
Volume (final)

V2

In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

  • Protocol 1

    Add each solvent one by one:  10% DMSO    40% PEG300    5% Tween-80    45% Saline

    Solubility: ≥ 2.17 mg/mL (3.51 mM); Clear solution

    This protocol yields a clear solution of ≥ 2.17 mg/mL (saturation unknown).

    Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (21.7 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.

    Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
  • Protocol 2

    Add each solvent one by one:  10% DMSO    90% Corn Oil

    Solubility: ≥ 2.17 mg/mL (3.51 mM); Clear solution

    This protocol yields a clear solution of ≥ 2.17 mg/mL (saturation unknown). If the continuous dosing period exceeds half a month, please choose this protocol carefully.

    Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (21.7 mg/mL) to 900 μL Corn oil, and mix evenly.

In Vivo Dissolution Calculator
Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

g

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Please enter your animal formula composition:
%
DMSO +
% 
+
%
Tween-80 +
%
Saline
Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).
Calculation results:
Working solution concentration: mg/mL
Method for preparing stock solution: mg drug dissolved in μL  DMSO (Stock solution concentration: mg/mL).
The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only. If necessary, please contact MedChemExpress (MCE).
Method for preparing in vivo working solution for animal experiments: Take μL DMSO stock solution, add μL . μL , mix evenly, next add μL Tween 80, mix evenly, then add μL Saline.
 If the continuous dosing period exceeds half a month, please choose this protocol carefully.
Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.
Purity & Documentation

Purity: 99.22%

SDS (251 KB)

COA (245 KB) LCMS (125 KB)

Handling Instructions (2659 KB)
References
Cell Assay
[1]

MDA-MB-231 and C2C12 cells are treated with 50μM of SIRT5 inhibitor MC3482 for 24 h. Ammonia levels are measured in the culture medium every other day[1].

MCE has not independently confirmed the accuracy of these methods. They are for reference only.
References

Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 2 years; -20°C, 1 year. When stored at -80°C, please use it within 2 years. When stored at -20°C, please use it within 1 year.

Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
DMSO 1 mM 1.6163 mL 8.0817 mL 16.1634 mL 40.4086 mL
5 mM 0.3233 mL 1.6163 mL 3.2327 mL 8.0817 mL
10 mM 0.1616 mL 0.8082 mL 1.6163 mL 4.0409 mL
15 mM 0.1078 mL 0.5388 mL 1.0776 mL 2.6939 mL
20 mM 0.0808 mL 0.4041 mL 0.8082 mL 2.0204 mL
25 mM 0.0647 mL 0.3233 mL 0.6465 mL 1.6163 mL
30 mM 0.0539 mL 0.2694 mL 0.5388 mL 1.3470 mL
40 mM 0.0404 mL 0.2020 mL 0.4041 mL 1.0102 mL
50 mM 0.0323 mL 0.1616 mL 0.3233 mL 0.8082 mL
60 mM 0.0269 mL 0.1347 mL 0.2694 mL 0.6735 mL
80 mM 0.0202 mL 0.1010 mL 0.2020 mL 0.5051 mL
100 mM 0.0162 mL 0.0808 mL 0.1616 mL 0.4041 mL
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MC3482 Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

MC34822922280-86-0MC 3482MC-3482SirtuinInhibitorinhibitorinhibit

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