MK-0773 (Synonyms: PF-05314882)

Name: MK-0773
Brand: MedChemExpress
SKU: HY-11027
Rating: 5.0 (4 reviews)

Cat. No.: HY-11027 Purity: 99.92%: FAQs
Data Sheet SDS COA Handling Instructions

Molarity Calculator

MK-0773 is a selective androgen receptor modulators (SARMs) that binds to AR with an IC₅₀ of 6.6 nM.

For research use only. We do not sell to patients.

MK-0773 Chemical Structure

CAS No. : 606101-58-0

Size	Price	Stock	Quantity

Free Sample (0.1 - 0.5 mg)		Apply Now
Solid + Solvent (Highly Recommended)
10 mM * 1 mL in DMSO ready for reconstitution	USD 245	In-stock	Estimated Time of Arrival: December 31
Solution
10 mM * 1 mL in DMSO	USD 245	In-stock	Estimated Time of Arrival: December 31
Solid
5 mg	USD 232	In-stock	Estimated Time of Arrival: December 31
10 mg	USD 353	In-stock	Estimated Time of Arrival: December 31
50 mg	USD 837	In-stock	Estimated Time of Arrival: December 31
100 mg	USD 1302	In-stock	Estimated Time of Arrival: December 31
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Customer Review

Based on 4 publication(s) in Google Scholar

4 Publications Citing Use of MCE MK-0773

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Biological Activity
Protocol
Purity & Documentation
References
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Description

MK-0773 is a selective androgen receptor modulators (SARMs) that binds to AR with an IC₅₀ of 6.6 nM.

IC₅₀ & Target

IC50: 6.6 nM (AR)

Cellular Effect

Cell Line	Type	Value	Description	References
CV-1	EC₅₀	144 nM Compound: PF-05314882	Agonist activity at human AR expressed in African green monkey CV1 cells after 17 hrs by ARE-driven luciferase reporter gene assay Agonist activity at human AR expressed in African green monkey CV1 cells after 17 hrs by ARE-driven luciferase reporter gene assay	10.1039/C3MD20341G

In Vitro

The IC₅₀ of MK-0773 binding to AR is increased 3.5-fold in the presence of 25% rat serum and 13-fold in the presence of 25% human serum, indicating that it binds to serum proteins. The affinity of MK-0773 for AR across species is evaluated using COS cells transfected with AR, and IC₅₀ values are very similar in four species (rat, 0.50 nM; dog, 0.55 nM; rhesus, 0.45 nM; human, 0.65 nM)^[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

In Vivo

MK-0773 (6 and 80 mg/kg, s.c.) produces exposure-related stimulatory effects on cortical BFR and LBM in the OVX rat model. MK-0773 (5, 15, and 80 mg/kg, s.c.) increases seminal vesicle weights, and has reduced effects on the prostate. The partial agonism and tissue selectivity of MK-0773 does not translate into differential effects on lipid metabolism in OVX rats^[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Clinical Trial

Molecular Weight

479.59

Formula

C₂₇H₃₄FN₅O₂

CAS No.

606101-58-0

Appearance

Solid

Color

Light yellow to yellow

SMILES

O=C([C@H]1CC[C@]2([H])[C@]1(C)CC[C@]3([H])[C@@]4(C)C=C(F)C(N(C)[C@]4([H])CC[C@]32[H])=O)NCC5=NC6=CC=CN=C6N5

Shipping

Room temperature in continental US; may vary elsewhere.

Storage

Powder	-20°C	3 years
	4°C	2 years
In solvent	-80°C	2 years
	-20°C	1 year

Solvent & Solubility

In Vitro:

DMSO : 33.33 mg/mL (69.50 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Preparing
Stock Solutions

Concentration Solvent Mass	1 mg	5 mg	10 mg

1 mM	2.0851 mL	10.4256 mL	20.8511 mL
5 mM	0.4170 mL	2.0851 mL	4.1702 mL
10 mM	0.2085 mL	1.0426 mL	2.0851 mL

View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 2 years; -20°C, 1 year. When stored at -80°C, please use it within 2 years. When stored at -20°C, please use it within 1 year.

Molarity Calculator
Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

This equation is commonly abbreviated as: C₁V₁ = C₂V₂

Concentration _(start)

Volume _(start)

Concentration _(final)

Volume _(final)

In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

Protocol 1

Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline
Solubility: ≥ 2.5 mg/mL (5.21 mM); Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.
Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
Protocol 2

Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in Saline)
Solubility: ≥ 2.5 mg/mL (5.21 mM); Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.
Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
Protocol 3

Add each solvent one by one: 10% DMSO 90% Corn Oil
Solubility: ≥ 2.5 mg/mL (5.21 mM); Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown). If the continuous dosing period exceeds half a month, please choose this protocol carefully.
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL Corn oil, and mix evenly.

In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Please enter your animal formula composition:

DMSO +

Tween-80 +

Saline

Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.

The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).

Calculation results:

Working solution concentration: mg/mL

Method for preparing stock solution: mg drug dissolved in μL DMSO (Stock solution concentration: mg/mL).

The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only. If necessary, please contact MedChemExpress (MCE).

Method for preparing in vivo working solution for animal experiments: Take μL DMSO stock solution, add μL . μL , mix evenly, next add μL Tween 80, mix evenly, then add μL Saline.

If the continuous dosing period exceeds half a month, please choose this protocol carefully.

Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.

Purity & Documentation

Purity: 99.92%

Select Batch:

Data Sheet (276 KB) SDS (252 KB)

COA (253 KB) LCMS (106 KB)

Handling Instructions (2659 KB)

References

[1]. Schmidt A, et al. Discovery of the selective androgen receptor modulator MK-0773 using a rational development strategy based on differential transcriptional requirements for androgenic anabolism versus reproductive physiology. J Biol Chem. 2010 May 28;285 [Content Brief]

Animal Administration
^[1]

Rats: Prostate and seminal vesicles are studied in 3-4-month-old 250-300-g rats after orchidectomy (ORX). Nine days after surgery, animals are injected (subcutaneously) daily with test compounds for 17 days, and the weight of the seminal vesicles (SVs) is compared with ORX rats treated with vehicle or DHT as a positive control.

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

References

[1]. Schmidt A, et al. Discovery of the selective androgen receptor modulator MK-0773 using a rational development strategy based on differential transcriptional requirements for androgenic anabolism versus reproductive physiology. J Biol Chem. 2010 May 28;285 [Content Brief]

Complete Stock Solution Preparation Table

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg

DMSO	1 mM	2.0851 mL	10.4256 mL	20.8511 mL	52.1279 mL
	5 mM	0.4170 mL	2.0851 mL	4.1702 mL	10.4256 mL
	10 mM	0.2085 mL	1.0426 mL	2.0851 mL	5.2128 mL
	15 mM	0.1390 mL	0.6950 mL	1.3901 mL	3.4752 mL
	20 mM	0.1043 mL	0.5213 mL	1.0426 mL	2.6064 mL
	25 mM	0.0834 mL	0.4170 mL	0.8340 mL	2.0851 mL
	30 mM	0.0695 mL	0.3475 mL	0.6950 mL	1.7376 mL
	40 mM	0.0521 mL	0.2606 mL	0.5213 mL	1.3032 mL
	50 mM	0.0417 mL	0.2085 mL	0.4170 mL	1.0426 mL
	60 mM	0.0348 mL	0.1738 mL	0.3475 mL	0.8688 mL

MK-0773 Related Classifications

Endocrinology

Help & FAQs

Do most proteins show cross-species activity?
Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

MK-0773606101-58-0PF-05314882MK0773MK 0773PF05314882PF 05314882Androgen ReceptorInhibitorinhibitorinhibit

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MK-0773 (Synonyms: PF-05314882)

Customer Review

MK-0773 Related Antibodies

4 Publications Citing Use of MCE MK-0773

Featured Recommendations

•Related Screening Libraries:

•Related Small Molecules:

•Related Proteins:

Biological Activity

Protocol

Purity & Documentation

References

Customer Review

MK-0773 Related Antibodies

Molarity Calculator

Dilution Calculator

Complete Stock Solution Preparation Table

MK-0773 Related Classifications

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