2. Metabolic Enzyme/Protease
  3. Dihydroorotate Dehydrogenase
  4. ML390

ML390 is a potent dihydroorotate dehydrogenase (DHODH) inhibitor. ML390 is an inducer of myeloid differentiation and causes myeloid differentiation in murine (ER-HoxA9) and human (U937 and THP1) acute myeloid leukemia (AML) models.

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ML390 Chemical Structure

ML390 Chemical Structure

CAS No. : 2029049-79-2

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10 mM * 1 mL in DMSO
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Customer Review

Based on 1 publication(s) in Google Scholar

ML390 Related Antibodies

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  • Purity & Documentation

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Description

ML390 is a potent dihydroorotate dehydrogenase (DHODH) inhibitor. ML390 is an inducer of myeloid differentiation and causes myeloid differentiation in murine (ER-HoxA9) and human (U937 and THP1) acute myeloid leukemia (AML) models[1][2][3].

Cellular Effect
Cell Line Type Value Description References
Bone marrow cell EC50
1.8 μM
Compound: 3G
Induction of bone marrow cell differentiation isolated from ER-HOXA9 fusion protein expressed mouse harboring GFP-lysozyme assessed as upregulation of CD11b/MAC1 after 4 days by flow cytometry
Induction of bone marrow cell differentiation isolated from ER-HOXA9 fusion protein expressed mouse harboring GFP-lysozyme assessed as upregulation of CD11b/MAC1 after 4 days by flow cytometry
[PMID: 27994748]
THP-1 EC50
6.5 μM
Compound: 3G
Induction of human THP1 cell differentiation after 4 days by flow cytometry
Induction of human THP1 cell differentiation after 4 days by flow cytometry
[PMID: 27994748]
U-937 EC50
8.8 μM
Compound: 3G
Induction of human U937 cell differentiation after 4 days by flow cytometry
Induction of human U937 cell differentiation after 4 days by flow cytometry
[PMID: 27994748]
In Vitro

ML390 is active with an ED50 of ~2 μM in murine and human AML cell lines. In vitro, treatment of Lys-GFP-ER-HoxA9 cells with ML390 for 48 hr inhibits DHODH activity, leading to the dramatic (>500-fold) accumulation of the upstream metabolite DHO and the depletion of uridine and other downstream metabolites[2].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

ML390 Related Antibodies
Molecular Weight

406.40

Formula

C21H21F3N2O3
CAS No.
Appearance

Solid

Color

White to off-white

SMILES

O=C(NCCC(N[C@H]1C(C=CC=C2)=C2CCC1)=O)C3=CC=C(OC(F)(F)F)C=C3
Shipping

Room temperature in continental US; may vary elsewhere.
Storage
Powder -20°C 3 years
4°C 2 years
In solvent -80°C 2 years
-20°C 1 year
Solvent & Solubility
In Vitro: 

DMSO : ≥ 27 mg/mL (66.44 mM; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

*"≥" means soluble, but saturation unknown.

Preparing
Stock Solutions
Concentration Solvent Mass 1 mg 5 mg 10 mg
1 mM 2.4606 mL 12.3032 mL 24.6063 mL
5 mM 0.4921 mL 2.4606 mL 4.9213 mL
View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 2 years; -20°C, 1 year. When stored at -80°C, please use it within 2 years. When stored at -20°C, please use it within 1 year.

  • Molarity Calculator

  • Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

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Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

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In Vivo Dissolution Calculator
Please enter the basic information of animal experiments:

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Calculation results:
Working solution concentration: mg/mL
Purity & Documentation
References

Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 2 years; -20°C, 1 year. When stored at -80°C, please use it within 2 years. When stored at -20°C, please use it within 1 year.

Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
DMSO 1 mM 2.4606 mL 12.3031 mL 24.6063 mL 61.5157 mL
5 mM 0.4921 mL 2.4606 mL 4.9213 mL 12.3031 mL
10 mM 0.2461 mL 1.2303 mL 2.4606 mL 6.1516 mL
15 mM 0.1640 mL 0.8202 mL 1.6404 mL 4.1010 mL
20 mM 0.1230 mL 0.6152 mL 1.2303 mL 3.0758 mL
25 mM 0.0984 mL 0.4921 mL 0.9843 mL 2.4606 mL
30 mM 0.0820 mL 0.4101 mL 0.8202 mL 2.0505 mL
40 mM 0.0615 mL 0.3076 mL 0.6152 mL 1.5379 mL
50 mM 0.0492 mL 0.2461 mL 0.4921 mL 1.2303 mL
60 mM 0.0410 mL 0.2051 mL 0.4101 mL 1.0253 mL
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ML390 Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

ML3902029049-79-2ML 390ML-390Dihydroorotate DehydrogenaseDHODHmyeloiddifferentiatioER-HoxA9U937THP1AMLInhibitorinhibitorinhibit

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