Madrasin (Synonyms: DDD00107587)

Name: Madrasin
Brand: MedChemExpress
SKU: HY-100236
Rating: 5.0 (7 reviews)

Cat. No.: HY-100236 Purity: 99.17%: FAQs
Data Sheet SDS COA Handling Instructions

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Madrasin (DDD00107587) is a splicing inhibitor that prevents formation of both splicing intermediates and products in vitro and interferes with one or more early steps in the pathway of spliceosome assembly. Madrasin also can inhibit pre-mRNA splicing in vitro and modify splicing of endogenous pre-mRNA in cells.

For research use only. We do not sell to patients.

Madrasin Chemical Structure

CAS No. : 374913-63-0

Size	Price	Stock	Quantity
5 mg	USD 60	In-stock	Estimated Time of Arrival: December 31
10 mg	USD 95	In-stock	Estimated Time of Arrival: December 31
25 mg	USD 150	In-stock	Estimated Time of Arrival: December 31
50 mg	USD 250	In-stock	Estimated Time of Arrival: December 31
100 mg	USD 450	In-stock	Estimated Time of Arrival: December 31
200 mg		Get quote
500 mg		Get quote

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Customer Review

Based on 7 publication(s) in Google Scholar

4 Publications Citing Use of MCE Madrasin

: Madrasin purchased from MedChemExpress. Usage Cited in: Nucleic Acids Res. 2019 Sep 5;47(15):8239-8254. [Abstract]; IF staining shows the loss of POLR2A protein after madrasin treatment. IF staining of POLR2A is performed after 24 h of Madrasin treatment at 30 uM. EU incorporation assay shows substantial reduction of newly transcribed RNA after Madrasin treatment. Assay is performed after 24 h of Madrasin treatment (30 μM).

: Madrasin purchased from MedChemExpress. Usage Cited in: Nucleic Acids Res. 2019 Sep 5;47(15):8239-8254. [Abstract]; Madrasin treatment leads to decrease of spliced POLR2A transcripts and accumulation of unspliced transcripts.

: Madrasin purchased from MedChemExpress. Usage Cited in: Nucleic Acids Res. 2019 Sep 5;47(15):8239-8254. [Abstract]; Western blot shows loss of POLR2A protein in Madrasin-treated cells. Cells are harvested after Madrasin treatment (30 μM) for 24 h.

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Description

IC₅₀ & Target

pre-mRNA splicing^[1]

Cellular Effect

Cell Line	Type	Value	Description	References
HeLa	EC₅₀	20 μM Compound: Madrasin	Inhibition of CLK mediated SF3B1 activation in human HeLa cells assessed as MDM2-pre mRNA exon skipping by luciferase reporter gene assay Inhibition of CLK mediated SF3B1 activation in human HeLa cells assessed as MDM2-pre mRNA exon skipping by luciferase reporter gene assay	[PMID: 28049589]

In Vitro

Madrasin (10-30 μM; 4-24 hours; HeLa cells) treatment inhibits the splicing of each of the RIOK3, BRD2, and Hsp40, MCL1, CCNA2, AURKA and p27 pre-mRNAs in both HeLa and HEK293 cells^[1].
Madrasin (10-30 μM; 4-24 hours; HeLa and HEK293 cells) treatment shows a dose- and time-dependent inhibitory effect on cell cycle progression^[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

RT-PCR^[1]

Cell Line:	HeLa cells
Concentration:	10 μM, 20 μM, or 30 μM
Incubation Time:	4 hours, 8 hours, or 24 hours
Result:	Increased in inhibition of pre-mRNA splicing.

Cell Cycle Analysis^[1]

Cell Line:	HeLa and HEK293 cells
Concentration:	10 μM, 20 μM, or 30 μM
Incubation Time:	4 hours, 8 hours, or 24 hours
Result:	In the presence of 10 μM 8 hours after treatment, the proportion of cells in G2, M, and S phases increased, with a concomitant decrease in the number of G1 phase cells. This effect increased over time, with >40% of cells in G2 and M phase and >50% in S phase 24 hours.

Molecular Weight

311.34

Formula

C₁₆H₁₇N₅O₂

CAS No.

374913-63-0

Appearance

Solid

Color

White to light yellow

SMILES

O=C1N=C(NC2=NC(C)=C3C=CC(OC)=CC3=N2)NC(C)=C1C

Shipping

Room temperature in continental US; may vary elsewhere.

Storage

Powder	-20°C	3 years
	4°C	2 years
In solvent	-80°C	2 years
	-20°C	1 year

Solvent & Solubility

In Vitro:

Ethanol : 2 mg/mL (6.42 mM; ultrasonic and warming and heat to 60°C)

DMSO : 2 mg/mL (6.42 mM; ultrasonic and warming and heat to 60°C; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Preparing
Stock Solutions

Concentration Solvent Mass	1 mg	5 mg	10 mg

1 mM	3.2119 mL	16.0596 mL	32.1192 mL
5 mM	0.6424 mL	3.2119 mL	6.4238 mL
10 mM	---	---	---

View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 2 years; -20°C, 1 year. When stored at -80°C, please use it within 2 years. When stored at -20°C, please use it within 1 year.

Molarity Calculator
Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

This equation is commonly abbreviated as: C₁V₁ = C₂V₂

Concentration _(start)

Volume _(start)

Concentration _(final)

Volume _(final)

In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

Protocol 1

Add each solvent one by one: 10% EtOH 40% PEG300 5% Tween-80 45% Saline
Solubility: ≥ 0.5 mg/mL (1.61 mM); Clear solution

This protocol yields a clear solution of ≥ 0.5 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL EtOH stock solution (5.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.
Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
Protocol 2

Add each solvent one by one: 10% EtOH 90% Corn Oil
Solubility: ≥ 0.5 mg/mL (1.61 mM); Clear solution

This protocol yields a clear solution of ≥ 0.5 mg/mL (saturation unknown). If the continuous dosing period exceeds half a month, please choose this protocol carefully.
Taking 1 mL working solution as an example, add 100 μL EtOH stock solution (5.0 mg/mL) to 900 μL Corn oil, and mix evenly.

For the following dissolution methods, please prepare the working solution directly. It is recommended to prepare fresh solutions and use them promptly within a short period of time.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

Protocol 1

Add each solvent one by one: 0.5% CMC-Na/saline water
Solubility: 12.5 mg/mL (40.15 mM); Suspended solution; Need ultrasonic

In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Please enter your animal formula composition:

DMSO +

Tween-80 +

Saline

Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.

The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).

Calculation results:

Working solution concentration: mg/mL

Method for preparing stock solution: mg drug dissolved in μL DMSO (Stock solution concentration: mg/mL).

The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only. If necessary, please contact MedChemExpress (MCE).

Method for preparing in vivo working solution for animal experiments: Take μL DMSO stock solution, add μL . μL , mix evenly, next add μL Tween 80, mix evenly, then add μL Saline.

If the continuous dosing period exceeds half a month, please choose this protocol carefully.

Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.

Purity & Documentation

Purity: 99.90%

Select Batch:

Data Sheet (273 KB) SDS (392 KB)

COA (246 KB) LCMS (267 KB)

Handling Instructions (2659 KB)

References

[1]. Andrea Pawellek et al. Identification of Small Molecule Inhibitors of Pre-mRNA Splicing. J Biol Chem, 2014 Dec 12, 289(50): 34683-34698. [Content Brief]

Complete Stock Solution Preparation Table

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg

Ethanol / DMSO	1 mM	3.2119 mL	16.0596 mL	32.1192 mL	80.2981 mL
Ethanol / DMSO	5 mM	0.6424 mL	3.2119 mL	6.4238 mL	16.0596 mL

Madrasin Related Classifications

Help & FAQs

Do most proteins show cross-species activity?
Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

Madrasin374913-63-0DDD00107587DDD 00107587DDD-00107587OthersInhibitorinhibitorinhibit

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Madrasin (Synonyms: DDD00107587)

Customer Review

Madrasin Related Antibodies

4 Publications Citing Use of MCE Madrasin

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•Related Screening Libraries:

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References

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Madrasin Related Antibodies

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Complete Stock Solution Preparation Table

Madrasin Related Classifications

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