Mertansine (Synonyms: DM1; Maytansinoid DM1)

Name: Mertansine
Brand: MedChemExpress
SKU: HY-19792
Rating: 5.0 (21 reviews)

Cat. No.: HY-19792 Purity: 99.55%: FAQs
Data Sheet SDS COA Handling Instructions

Molarity Calculator

Mertansine (DM1) is a microtubulin inhibitor and is an antibody-conjugatable maytansinoid that is developed to overcome systemic toxicity associated with maytansine and to enhance tumor-specific delivery. Mertansine can be attached to a monoclonal antibody with a linker to create an antibody-drug conjugate (ADC).

For research use only. We do not sell to patients.

Mertansine Chemical Structure

CAS No. : 139504-50-0

Size	Price	Stock	Quantity
Free Sample (0.1 - 0.5 mg)		Apply Now
2 mg	USD 50	In-stock	Estimated Time of Arrival: December 31
5 mg	USD 80	In-stock	Estimated Time of Arrival: December 31
10 mg	USD 125	In-stock	Estimated Time of Arrival: December 31
50 mg	USD 250	In-stock	Estimated Time of Arrival: December 31
100 mg	USD 400	In-stock	Estimated Time of Arrival: December 31
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Customer Review

Based on 21 publication(s) in Google Scholar

20 Publications Citing Use of MCE Mertansine

: Mertansine purchased from MedChemExpress. Usage Cited in: Biomaterials. 2022: 121913.; Free Mertansine (DM1; 20 nM; 24 h) is able to induce apoptosis in NCI-H929 cells.

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Biological Activity
Purity & Documentation
References
Customer Review

Description

IC₅₀ & Target

Maytansinoids

In Vitro

Mertansine is a strong antiproliferative chemotherapeutics toward over 60 types of cancer cell lines^[3].
Mertansine (0-1 μg/mL) shows antitumor activity in malignant B16F10 melanoma cells, and inhibits tumor cell growth by inhibiting mitosis when combined with DTX^[3].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Cell Proliferation Assay

Cell Line:	Malignant B16F10 melanoma cells^[3]
Concentration:	0, 0.01, 0.1, and 1 μg/mL
Incubation Time:	4 h
Result:	Showed antitumor activity in malignant B16F10 melanoma cells, with an IC₅₀ of 0.092 μg/mL. Co-delivery of DTX and DM1, both of which inhibit tumor cell growth by inhibiting mitosis, is an effective strategy to achieve a combinatorial anticancer effect.

In Vivo

Mertansine (DM1) has a low maximum-tolerated dose (MTD) of 1 mg/kg^[3]

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Clinical Trial

Molecular Weight

738.29

Formula

C₃₅H₄₈ClN₃O₁₀S

CAS No.

139504-50-0

Appearance

Solid

Color

White to light yellow

SMILES

C[C@]1([C@@](CC(N(C(C=C2C=C3OC)=C3Cl)C)=O)([H])OC([C@H](C)N(C)C(CCS)=O)=O)[C@H]([C@@H]([C@](OC4=O)([H])C[C@]([C@](/C=C/C=C(C)/C2)([H])OC)(N4)O)C)O1

Shipping

Room temperature in continental US; may vary elsewhere.

Storage

Powder	-20°C	3 years
	4°C	2 years

*The compound is unstable in solutions, freshly prepared is recommended.

Solvent & Solubility

In Vitro:

DMSO : 62.5 mg/mL (84.66 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Preparing
Stock Solutions

Concentration Solvent Mass	1 mg	5 mg	10 mg

1 mM	1.3545 mL	6.7724 mL	13.5448 mL
5 mM	0.2709 mL	1.3545 mL	2.7090 mL
10 mM	0.1354 mL	0.6772 mL	1.3545 mL

View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. The compound is unstable in solutions, freshly prepared is recommended.

Molarity Calculator
Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

This equation is commonly abbreviated as: C₁V₁ = C₂V₂

Concentration _(start)

Volume _(start)

Concentration _(final)

Volume _(final)

In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

Protocol 1

Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in Saline)
Solubility: 2.17 mg/mL (2.94 mM); Suspended solution; Need ultrasonic

This protocol yields a suspended solution of 2.17 mg/mL. Suspended solution can be used for oral and intraperitoneal injection.
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (21.7 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.
Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
Protocol 2

Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline
Solubility: ≥ 2.08 mg/mL (2.82 mM); Clear solution

This protocol yields a clear solution of ≥ 2.08 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (20.8 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.
Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
Protocol 3

Add each solvent one by one: 10% DMSO 90% Corn Oil
Solubility: ≥ 2.08 mg/mL (2.82 mM); Clear solution

This protocol yields a clear solution of ≥ 2.08 mg/mL (saturation unknown). If the continuous dosing period exceeds half a month, please choose this protocol carefully.
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (20.8 mg/mL) to 900 μL Corn oil, and mix evenly.

In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Please enter your animal formula composition:

DMSO +

Tween-80 +

Saline

Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.

The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).

Calculation results:

Working solution concentration: mg/mL

Method for preparing stock solution: mg drug dissolved in μL DMSO (Stock solution concentration: mg/mL).

*The compound is unstable in solutions, freshly prepared is recommended.

The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only. If necessary, please contact MedChemExpress (MCE).

Method for preparing in vivo working solution for animal experiments: Take μL DMSO stock solution, add μL . μL , mix evenly, next add μL Tween 80, mix evenly, then add μL Saline.

If the continuous dosing period exceeds half a month, please choose this protocol carefully.

Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.

Purity & Documentation

Purity: 99.80%

Select Batch:

Data Sheet (283 KB) SDS (393 KB)

COA (259 KB) LCMS (112 KB)

Handling Instructions (2659 KB)

References

[1]. Manu Lopus et al. Maytansine and Cellular Metabolites of Antibody-Maytansinoid Conjugates Strongly Suppress Microtubule Dynamics by Binding to Microtubules. [Content Brief]

[2]. Lopus M. Antibody-DM1 conjugates as cancer therapeutics. Cancer Lett. 2011 Aug 28;307(2):113-8. [Content Brief]

[3]. Zhong P, et al. cRGD-installed docetaxel-loaded mertansine prodrug micelles: redox-triggered ratiometric dual drug release and targeted synergistic treatment of B16F10 melanoma. Nanotechnology. 2017 Jul 21;28(29):295103. [Content Brief]

Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. The compound is unstable in solutions, freshly prepared is recommended.

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg

DMSO	1 mM	1.3545 mL	6.7724 mL	13.5448 mL	33.8620 mL
	5 mM	0.2709 mL	1.3545 mL	2.7090 mL	6.7724 mL
	10 mM	0.1354 mL	0.6772 mL	1.3545 mL	3.3862 mL
	15 mM	0.0903 mL	0.4515 mL	0.9030 mL	2.2575 mL
	20 mM	0.0677 mL	0.3386 mL	0.6772 mL	1.6931 mL
	25 mM	0.0542 mL	0.2709 mL	0.5418 mL	1.3545 mL
	30 mM	0.0451 mL	0.2257 mL	0.4515 mL	1.1287 mL
	40 mM	0.0339 mL	0.1693 mL	0.3386 mL	0.8466 mL
	50 mM	0.0271 mL	0.1354 mL	0.2709 mL	0.6772 mL
	60 mM	0.0226 mL	0.1129 mL	0.2257 mL	0.5644 mL
	80 mM	0.0169 mL	0.0847 mL	0.1693 mL	0.4233 mL

Mertansine Related Classifications

Help & FAQs

Do most proteins show cross-species activity?
Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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Mertansine (Synonyms: DM1; Maytansinoid DM1)

Customer Review

Mertansine Related Antibodies

20 Publications Citing Use of MCE Mertansine

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•Related Screening Libraries:

•Related Small Molecules:

•Related Proteins:

View All ADC Cytotoxin Isoform Specific Products:

Biological Activity

Purity & Documentation

References

Customer Review

Mertansine Related Antibodies

Molarity Calculator

Dilution Calculator

Complete Stock Solution Preparation Table

Mertansine Related Classifications

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