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  4. Nile Red

Nile Red  (Synonyms: Nile Blue A oxazone; Phenoxazone 9)

Cat. No.: HY-D0718 Purity: 98.08%
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Nile red (Nile blue oxazone) is a lipophilic stain. Nile red has environment-sensitive fluorescence. Nile red is intensely fluorescent in a lipid-rich environment while it has minimal fluorescence in aqueous media. Nile red is an excellent vital stain for the detection of intracellular lipid droplets by fluorescence microscopy and flow cytof uorometry. Nile red stains intracellular lipid droplets red. The fluorescence wavelength is 559/635 nm.

For research use only. We do not sell to patients.

Nile Red Chemical Structure

Nile Red Chemical Structure

CAS No. : 7385-67-3

Size Price Stock Quantity
5 mg USD 35 In-stock
10 mg USD 55 In-stock
50 mg USD 72 In-stock
100 mg USD 83 In-stock
500 mg USD 132 In-stock
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This product is a controlled substance and not for sale in your territory.

Customer Review

Based on 69 publication(s) in Google Scholar

Nile Red Related Antibodies

Top Publications Citing Use of Products

59 Publications Citing Use of MCE Nile Red

IF

    Nile Red purchased from MedChemExpress. Usage Cited in: Nano Today. 47 (2022) 101675

    Subcellular biodistribution of NileRed-labeled PFD Liposome nano-particles in 4T1 and BT-549 cells are visualized by confocal laser microscopy. Blue, DAPI; Green, WGA 488; Red, NileRed-labeled PFD Liposome.

    Nile Red purchased from MedChemExpress. Usage Cited in: Nat Commun. 2022 Oct 5;13(1):5871.  [Abstract]

    Isolated oocytes are fixed with 4% paraformaldehyde for 1 hour at room temperature and washed 3 times with 1% BSA in PBS. Then, they are stained with 10 µg/ml Nile red at 4 °C overnight.

    Nile Red purchased from MedChemExpress. Usage Cited in: Nucleic Acids Res. 2022 Jun 24;50(12):6953-6967.  [Abstract]

    The wild-type or G4-mutant human hepatic adenocarcinoma HepG2 cells are stained with Nile Red reagent to visualize the lipids with an Olympus Fluoview FV1000 confocal microscope.

    Nile Red purchased from MedChemExpress. Usage Cited in: EMBO J. 2022 Jul 4;e110439.  [Abstract]

    Representative pictures of Nile red-stained human BAs differentiated from hESC-derived brown progenitors.

    Nile Red purchased from MedChemExpress. Usage Cited in: J Transl Med. 2022 May 14;20(1):222.  [Abstract]

    Living cells are incubated with Nile Red (1 μM) for 15 min at 37 °C in dark. Then, cells are washed with HBSS/Ca/Mg and analyzed by fluorescence microscopy.

    Nile Red purchased from MedChemExpress. Usage Cited in: Eur J Pharmacol. 2022 Nov 24;175428.  [Abstract]

    Hepatic lipid and FFA content are determined by Nile red staining of liver frozen sections.

    Nile Red purchased from MedChemExpress. Usage Cited in: Theranostics. 2021 Jan 1;11(5):2149-2169.  [Abstract]

    Injected 5 μg of Nile Red lipid dye into the tail vein of mice to label triglycerides in the liver prior to intravital imaging.

    Nile Red purchased from MedChemExpress. Usage Cited in: Cell Prolif. 2021 Sep 25;e13134.  [Abstract]

    For lipid droplet staining, fixed cells are stained for 30 minutes with 0.1 µg/ml Nile Red (MCE), and the nucleus is counterstained using DAPI.

    Nile Red purchased from MedChemExpress. Usage Cited in: Int J Mol Sci. 2021 May 31;22(11):5951.  [Abstract]

    For lipid staining, fat bodies are submerged in Nile red solution at a final working concentration of 10 μg/mL in an acetone/water (1:9) mixture and visualized using a fluorescent microscope at Ex543/Em626 nm.

    Nile Red purchased from MedChemExpress. Usage Cited in: Nat Commun. 2020 Jan 13;11(1):240.   [Abstract]

    HSCs are stained with Nile Red reagents and Bodipy493/503 to visualize the lipids with a light microscope.

    • Biological Activity

    • Purity & Documentation

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    Description

    Nile red (Nile blue oxazone) is a lipophilic stain. Nile red has environment-sensitive fluorescence. Nile red is intensely fluorescent in a lipid-rich environment while it has minimal fluorescence in aqueous media. Nile red is an excellent vital stain for the detection of intracellular lipid droplets by fluorescence microscopy and flow cytof uorometry. Nile red stains intracellular lipid droplets red. The fluorescence wavelength is 559/635 nm[1].

    In Vitro

    1.1 Preparation of stock solution
    Use DMSO to prepare 1 mM stock solution.
    1.2 Preparation of working solution
    Use preheated serum-free cell culture medium or PBS to dilute the stock solution to a final concentration of 200-1000 nM.
    Note: Please adjust the concentration of Nile Red working solution according to the actual situation and prepare it before use.
    2. Cell staining
    2.1 Suspended cells: Collect cells by centrifugation and wash twice with PBS for 5 minutes each time.
    Adherent cells: Discard the culture medium and add trypsin to digest the cells. After centrifugation and discarding the supernatant, wash twice with PBS for 5 minutes each time.
    2.2 Add 1 mL Nile Red working solution and incubate at room temperature for 5-10 minutes.
    2.3 Centrifuge at 400 g, 4℃ for 3-4 minutes and discard the supernatant.
    2.4 Wash cells twice with PBS for 5 minutes each time.
    2.5 Resuspend the cells in 1 mL serum-free medium or PBS and observe under a fluorescence microscope.

    MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

    Nile Red Related Antibodies
    In Vivo

    When Nile red-stained Caenorhabditis elegans is viewed for green fluorescence, discrete lipid bodies can be observed throughout the intestine and other tissues either in clusters or evenly dispersed, depending on the animal's genotype or experimental treatment[3].

    MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
    Molecular Weight

    318.37

    Formula

    C20H18N2O2
    CAS No.
    Appearance

    Solid

    Color

    Green to dark green

    Emission (Em)

    598
    Excitation (Ex)

    543
    SMILES

    O=C1C2=CC=CC=C2C3=NC4=CC=C(N(CC)CC)C=C4OC3=C1
    Shipping

    Room temperature in continental US; may vary elsewhere.
    Storage

    4°C, protect from light

    *In solvent : -80°C, 2 years; -20°C, 1 year (protect from light)

    Solvent & Solubility
    In Vitro: 

    DMSO : 2 mg/mL (6.28 mM; ultrasonic and warming and heat to 60°C; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

    Ethanol : 1 mg/mL (3.14 mM; ultrasonic and warming and heat to 60°C)

    Preparing
    Stock Solutions
    Concentration Solvent Mass 1 mg 5 mg 10 mg
    1 mM 3.1410 mL 15.7050 mL 31.4100 mL
    5 mM 0.6282 mL 3.1410 mL 6.2820 mL
    View the Complete Stock Solution Preparation Table

    * Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
    Storage method and period of stock solution: -80°C, 2 years; -20°C, 1 year (protect from light). When stored at -80°C, please use it within 2 years. When stored at -20°C, please use it within 1 year.

    • Molarity Calculator

    • Dilution Calculator

    Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

    Mass
    =
    Concentration
    ×
    Volume
    ×
    Molecular Weight *

    Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

    This equation is commonly abbreviated as: C1V1 = C2V2

    Concentration (start)

    C1

    ×
    Volume (start)

    V1

    =
    Concentration (final)

    C2

    ×
    Volume (final)

    V2

    In Vivo:

    Select the appropriate dissolution method based on your experimental animal and administration route.

    For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
    To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
    The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

    • Protocol 1

      Add each solvent one by one:  10% DMSO    40% PEG300    5% Tween-80    45% Saline

      Solubility: 0.2 mg/mL (0.63 mM); Suspended solution; Need ultrasonic

      This protocol yields a suspended solution of 0.2 mg/mL. Suspended solution can be used for oral and intraperitoneal injection.

      Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (2.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.

      Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
    • Protocol 2

      Add each solvent one by one:  10% DMSO    90% (20% SBE-β-CD in Saline)

      Solubility: ≥ 0.2 mg/mL (0.63 mM); Clear solution

      This protocol yields a clear solution of ≥ 0.2 mg/mL (saturation unknown).

      Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (2.0 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.

      Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
    In Vivo Dissolution Calculator
    Please enter the basic information of animal experiments:

    Dosage

    mg/kg

    Animal weight
    (per animal)

    g

    Dosing volume
    (per animal)

    μL

    Number of animals

    Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
    Please enter your animal formula composition:
    %
    DMSO +
    % 
    +
    %
    Tween-80 +
    %
    Saline
    Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
    The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).
    Calculation results:
    Working solution concentration: mg/mL
    Method for preparing stock solution: mg drug dissolved in μL  DMSO (Stock solution concentration: mg/mL).

    *In solvent : -80°C, 2 years; -20°C, 1 year (protect from light)

    The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only. If necessary, please contact MedChemExpress (MCE).
    Method for preparing in vivo working solution for animal experiments: Take μL DMSO stock solution, add μL . μL , mix evenly, next add μL Tween 80, mix evenly, then add μL Saline.
     If the continuous dosing period exceeds half a month, please choose this protocol carefully.
    Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.
    Purity & Documentation

    Purity: 98.15%

    SDS (393 KB)

    COA (250 KB) HNMR (257 KB) LCMS (268 KB)

    Handling Instructions (2659 KB)
    Dyeing Example
    References

    Complete Stock Solution Preparation Table

    * Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
    Storage method and period of stock solution: -80°C, 2 years; -20°C, 1 year (protect from light). When stored at -80°C, please use it within 2 years. When stored at -20°C, please use it within 1 year.

    Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
    Ethanol / DMSO 1 mM 3.1410 mL 15.7050 mL 31.4100 mL 78.5250 mL
    DMSO 5 mM 0.6282 mL 3.1410 mL 6.2820 mL 15.7050 mL
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    Nile Red Related Classifications

    Help & FAQs
    • Do most proteins show cross-species activity?

      Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

    Keywords:

    Nile Red7385-67-3Nile Blue A oxazone Phenoxazone 9Phenoxazone9Phenoxazone 9Phenoxazone-9Fluorescent DyeBenzophenoxazonehydrophobiclipiddropletsfluorescentneutrallysochromeInhibitorinhibitorinhibit

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