Orexin 2 Receptor Agonist

Name: Orexin 2 Receptor Agonist
Brand: MedChemExpress
SKU: HY-19320
Rating: 5.0 (1 reviews)

Cat. No.: HY-19320 Purity: 99.59%: FAQs
Data Sheet SDS COA Handling Instructions

Molarity Calculator

Orexin 2 Receptor Agonist is a potent selective OX2R agonist with an EC₅₀ of 23 nM.

For research use only. We do not sell to patients.

Orexin 2 Receptor Agonist Chemical Structure

CAS No. : 1796565-52-0

Size	Price	Stock	Quantity
Solid + Solvent (Highly Recommended)
10 mM * 1 mL in DMSO ready for reconstitution	USD 155	In-stock	Estimated Time of Arrival: December 31
Solution
10 mM * 1 mL in DMSO	USD 155	In-stock	Estimated Time of Arrival: December 31
Solid
5 mg	USD 120	In-stock	Estimated Time of Arrival: December 31
10 mg	USD 150	In-stock	Estimated Time of Arrival: December 31
25 mg	USD 255	In-stock	Estimated Time of Arrival: December 31
50 mg	USD 380	In-stock	Estimated Time of Arrival: December 31
100 mg	USD 550	In-stock	Estimated Time of Arrival: December 31
200 mg		Get quote
500 mg		Get quote

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Customer Review

Based on 1 publication(s) in Google Scholar

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OX₂ Receptor OX₁ Receptor

Biological Activity
Purity & Documentation
References
Customer Review

Description

Orexin 2 Receptor Agonist is a potent selective OX2R agonist with an EC₅₀ of 23 nM^[1].

IC₅₀ & Target

OX₂ Receptor

Cellular Effect

Cell Line	Type	Value	Description	References
CHO-K1	EC₅₀	0.023 μM Compound: 26	Agonist activity at human OX2R expressed in CHOK1 cells by NFAT-response luciferase reporter gene assay Agonist activity at human OX2R expressed in CHOK1 cells by NFAT-response luciferase reporter gene assay	[PMID: 26267383]
CHO-K1	EC₅₀	1.616 μM Compound: 26	Agonist activity at human OX1R expressed in CHOK1 cells by calcium mobilization assay Agonist activity at human OX1R expressed in CHOK1 cells by calcium mobilization assay	[PMID: 26267383]

In Vitro

Orexin 2 Receptor Agonist replaces Orexin-A to bind to hOX1R and hOX2R in a concentration-dependent manner in CHO cells overexpressing hOX1R and HEK-293 cells overexpressing hOX2R with K_i values of 0.14 μM and 0.77 μM, respectively^[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Molecular Weight

586.70

Formula

C₃₂H₃₄N₄O₅S

CAS No.

1796565-52-0

Appearance

Solid

Color

White to off-white

SMILES

O=C(C1=CC=CC(C)=C1)NCCNC2=CC(NS(C3=C(OC)C=CC(C4=CC(C(N(C)C)=O)=CC=C4)=C3)(=O)=O)=CC=C2

Shipping

Room temperature in continental US; may vary elsewhere.

Storage

Powder	-20°C	3 years
	4°C	2 years
In solvent	-80°C	2 years
	-20°C	1 year

Solvent & Solubility

In Vitro:

DMSO : ≥ 32 mg/mL (54.54 mM; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

*"≥" means soluble, but saturation unknown.

Preparing
Stock Solutions

Concentration Solvent Mass	1 mg	5 mg	10 mg

1 mM	1.7044 mL	8.5222 mL	17.0445 mL
5 mM	0.3409 mL	1.7044 mL	3.4089 mL
10 mM	0.1704 mL	0.8522 mL	1.7044 mL

View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 2 years; -20°C, 1 year. When stored at -80°C, please use it within 2 years. When stored at -20°C, please use it within 1 year.

Molarity Calculator
Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

This equation is commonly abbreviated as: C₁V₁ = C₂V₂

Concentration _(start)

Volume _(start)

Concentration _(final)

Volume _(final)

In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

Protocol 1

Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline
Solubility: ≥ 2.5 mg/mL (4.26 mM); Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.
Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
Protocol 2

Add each solvent one by one: 10% DMSO 90% Corn Oil
Solubility: ≥ 2.5 mg/mL (4.26 mM); Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown). If the continuous dosing period exceeds half a month, please choose this protocol carefully.
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL Corn oil, and mix evenly.

In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Please enter your animal formula composition:

DMSO +

Tween-80 +

Saline

Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.

The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).

Calculation results:

Working solution concentration: mg/mL

Method for preparing stock solution: mg drug dissolved in μL DMSO (Stock solution concentration: mg/mL).

The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only. If necessary, please contact MedChemExpress (MCE).

Method for preparing in vivo working solution for animal experiments: Take μL DMSO stock solution, add μL . μL , mix evenly, next add μL Tween 80, mix evenly, then add μL Saline.

If the continuous dosing period exceeds half a month, please choose this protocol carefully.

Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.

Purity & Documentation

Purity: 99.59%

Select Batch:

Data Sheet (270 KB) SDS (393 KB)

COA (246 KB) LCMS (93 KB)

Handling Instructions (2659 KB)

References

[1]. Nagahara T, et al. Design and Synthesis of Non-Peptide, Selective Orexin Receptor 2 Agonists. J Med Chem. 2015 Oct 22;58(20):7931-7. [Content Brief]

Complete Stock Solution Preparation Table

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg

DMSO	1 mM	1.7044 mL	8.5222 mL	17.0445 mL	42.6112 mL
	5 mM	0.3409 mL	1.7044 mL	3.4089 mL	8.5222 mL
	10 mM	0.1704 mL	0.8522 mL	1.7044 mL	4.2611 mL
	15 mM	0.1136 mL	0.5681 mL	1.1363 mL	2.8407 mL
	20 mM	0.0852 mL	0.4261 mL	0.8522 mL	2.1306 mL
	25 mM	0.0682 mL	0.3409 mL	0.6818 mL	1.7044 mL
	30 mM	0.0568 mL	0.2841 mL	0.5681 mL	1.4204 mL
	40 mM	0.0426 mL	0.2131 mL	0.4261 mL	1.0653 mL
	50 mM	0.0341 mL	0.1704 mL	0.3409 mL	0.8522 mL