Pamidronic acid

Name: Pamidronic acid
Brand: MedChemExpress
SKU: HY-B0012
Rating: 4.5 (1 reviews)

Cat. No.: HY-B0012 Purity: 98.15%: FAQs
Data Sheet SDS COA Handling Instructions

Molarity Calculator

Pamidronic acid, the second-generation nitrogen-containing bisphosphonate, is an inhibitor of bone loss. Pamidronic acid significantly inhibits subchondral bone loss in early osteoarthritis by upregulating the expression of OPG in cartilage and subchondral bone, and inhibiting the expression of RANKL and MMP-9 in both tissues, as well as TLR-4 in cartilage, thereby alleviating cartilage degeneration. Additionally, Pamidronic acid can inhibit the signaling of Wnt and β-catenin, and is applicable for research on osteoporosis and osteosarcoma.

For research use only. We do not sell to patients.

Pamidronic acid Chemical Structure

CAS No. : 40391-99-9

Size	Price	Stock	Quantity
Solid + Solvent (Highly Recommended)
10 mM * 1 mL in Water ready for reconstitution	USD 42	In-stock	Estimated Time of Arrival: December 31
Solution
10 mM * 1 mL in Water	USD 42	In-stock	Estimated Time of Arrival: December 31
Solid
25 mg	USD 38	In-stock	Estimated Time of Arrival: December 31
50 mg	USD 60	In-stock	Estimated Time of Arrival: December 31
100 mg	USD 96	In-stock	Estimated Time of Arrival: December 31
200 mg		Get quote
500 mg		Get quote

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Based on 1 publication(s) in Google Scholar

Other Forms of Pamidronic acid:

Pamidronate disodium pentahydrate In-stock
Pamidronate disodium Get quote
Pamidronic acid sodium Get quote

1 Publications Citing Use of MCE Pamidronic acid

•Cell Rep Methods. 2023 Oct 23;3(10):100599. [Abstract]

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TLR3 TLR8 TLR9 TLR2 TLR4 TLR7 TLR1 TLR6

Biological Activity
Protocol
Purity & Documentation
References
Customer Review

Description

Cellular Effect

Cell Line	Type	Value	Description	References
HepG2	IC₅₀	> 200 μM Compound: Pamidronate	Antimalarial activity against Plasmodium berghei NK65 infected in human HepG2 cells after 48 hrs by RT-PCR Antimalarial activity against Plasmodium berghei NK65 infected in human HepG2 cells after 48 hrs by RT-PCR	[PMID: 20457823]
MCF7	IC₅₀	141 μM Compound: 38	Antiproliferative activity against human MCF7 cell line by MTT assay Antiproliferative activity against human MCF7 cell line by MTT assay	[PMID: 16970405]
MCF7	IC₅₀	22.9 μM Compound: Pamidronate	Cytotoxicity against human MCF7 cells assessed as cell growth inhibition measured after 24 to 48 hrs by SRB assay Cytotoxicity against human MCF7 cells assessed as cell growth inhibition measured after 24 to 48 hrs by SRB assay	[PMID: 33132173]
NCI-H460	IC₅₀	71.8 μM Compound: 38	Antiproliferative activity against human NCI-H460 cell line by MTT assay Antiproliferative activity against human NCI-H460 cell line by MTT assay	[PMID: 16970405]
SF-268	IC₅₀	408 μM Compound: 38	Antiproliferative activity against human SF-268 cell line by MTT assay Antiproliferative activity against human SF-268 cell line by MTT assay	[PMID: 16970405]
T-cell	IC₅₀	62 μM Compound: 34	Inhibitory activity, for stimulation of TNF-alpha release in gamma-delta T cells, using individual observed maximum TNF-alpha release Inhibitory activity, for stimulation of TNF-alpha release in gamma-delta T cells, using individual observed maximum TNF-alpha release	[PMID: 14711309]
T-cell	IC₅₀	941 μM Compound: 34	Inhibitory activity, for stimulation of TNF-alpha release in gamma-delta T cells, using a constrained maximum TNF-alpha release of 2700 pg/mL Inhibitory activity, for stimulation of TNF-alpha release in gamma-delta T cells, using a constrained maximum TNF-alpha release of 2700 pg/mL	[PMID: 14711309]

Clinical Trial

Molecular Weight

235.07

Formula

C₃H₁₁NO₇P₂

CAS No.

40391-99-9

Appearance

Solid

Color

White to off-white

SMILES

OC(P(O)(O)=O)(P(O)(O)=O)CCN

Shipping

Room temperature in continental US; may vary elsewhere.

Storage

-20°C, protect from light

*In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)

Solvent & Solubility

In Vitro:

H₂O : 5 mg/mL (21.27 mM; ultrasonic and warming and heat to 80°C)

DMSO : < 1 mg/mL (insoluble or slightly soluble)

Preparing
Stock Solutions

Concentration Solvent Mass	1 mg	5 mg	10 mg

1 mM	4.2541 mL	21.2703 mL	42.5405 mL
5 mM	0.8508 mL	4.2541 mL	8.5081 mL
10 mM	0.4254 mL	2.1270 mL	4.2541 mL

View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

Molarity Calculator
Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

This equation is commonly abbreviated as: C₁V₁ = C₂V₂

Concentration _(start)

Volume _(start)

Concentration _(final)

Volume _(final)

In Vivo:

For the following dissolution methods, please prepare the working solution directly. It is recommended to prepare fresh solutions and use them promptly within a short period of time.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

Protocol 1

Add each solvent one by one: PBS
Solubility: 2 mg/mL (8.51 mM); Clear solution; Need ultrasonic

In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

Dosage

mg/kg

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(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Calculation results:

Working solution concentration: mg/mL

The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only.If necessary, please contact MedChemExpress (MCE).

Purity & Documentation

Purity: 98.15%

Select Batch:

Data Sheet (278 KB) SDS (393 KB)

COA (251 KB) HNMR (127 KB) RP-HPLC (220 KB)

Handling Instructions (2659 KB)

References

[1]. Ashton JA, et al. Investigation of the effect of pamidronate disodium on the in vitro viability of osteosarcoma cellsfrom dogs. Am J Vet Res. 2005 May;66(5):885-92. [Content Brief]

[2]. Xu Y, et al. Pamidronate Disodium Leads to Bone Necrosis via Suppression of Wnt/β-Catenin Signaling in Human Bone Marrow Mesenchymal Stem Cells In Vitro. J Oral Maxillofac Surg. 2017 Mar 23. [Content Brief]

[3]. Lv Y, et al. Effects of pamidronate disodium on the loss of osteoarthritic subchondral bone and the expression of cartilaginous and subchondral osteoprotegerin and RANKL in rabbits. BMC Musculoskelet Disord. 2014 Nov 6;15:371. [Content Brief]

[4]. Kovacs R, et al. The synthesis of pamidronic derivatives in different solvents: An optimization and a mechanistic study[J]. Heteroatom Chemistry, 2014, 25(3): 186-194.

Cell Assay ^[1]	Cell counts and cell viability assays are performed in cultures of osteosarcoma cells (POS, HMPOS, and COS31 cell lines) and fibroblasts after 24, 48, and 72 hours of incubation with pamidronate at concentrations of 0.001 to 1000 microM or with no drug (control treatment). Percentage viability is determined in cell samples for each concentration of pamidronate and each incubation time. A DNA fragmentation analysis is performed to assess bisphosphonate-induced apoptosis^[1]. MCE has not independently confirmed the accuracy of these methods. They are for reference only.
Animal Administration ^[3]	Rabbits: The rabbits are randomly divided into four groups. Sham-operated with vehicle treatment, OA induced by ACLT with vehicle treatment, OA-induced ACLT treated with short-term pamidronic acid treatment after ACLT, and ACLT treated with long-term PAM treatment. PAM is injected at the 4th week after ACLT in PAM-S and PAM-L groups, and followed by once monthly ear vein injections at a dosage of 3 mg/kg body weight. In the other groups, only saline infusions of equal volumes are administered. 10 animals are humanely sacrificed at both 2 and 10 weeks after pamidronic acid treatment. In the ACLT and Sham groups, five animals are sacrificed at 2, 4, 6, and 14 weeks after model establishment^[3]. MCE has not independently confirmed the accuracy of these methods. They are for reference only.
References	[1]. Ashton JA, et al. Investigation of the effect of pamidronate disodium on the in vitro viability of osteosarcoma cellsfrom dogs. Am J Vet Res. 2005 May;66(5):885-92. [Content Brief] [2]. Xu Y, et al. Pamidronate Disodium Leads to Bone Necrosis via Suppression of Wnt/β-Catenin Signaling in Human Bone Marrow Mesenchymal Stem Cells In Vitro. J Oral Maxillofac Surg. 2017 Mar 23. [Content Brief] [3]. Lv Y, et al. Effects of pamidronate disodium on the loss of osteoarthritic subchondral bone and the expression of cartilaginous and subchondral osteoprotegerin and RANKL in rabbits. BMC Musculoskelet Disord. 2014 Nov 6;15:371. [Content Brief] [4]. Kovacs R, et al. The synthesis of pamidronic derivatives in different solvents: An optimization and a mechanistic study[J]. Heteroatom Chemistry, 2014, 25(3): 186-194.

Complete Stock Solution Preparation Table

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg

H₂O	1 mM	4.2541 mL	21.2703 mL	42.5405 mL	106.3513 mL
	5 mM	0.8508 mL	4.2541 mL	8.5081 mL	21.2703 mL
	10 mM	0.4254 mL	2.1270 mL	4.2541 mL	10.6351 mL
	15 mM	0.2836 mL	1.4180 mL	2.8360 mL	7.0901 mL
	20 mM	0.2127 mL	1.0635 mL	2.1270 mL	5.3176 mL

* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

Pamidronic acid Related Classifications

Help & FAQs

Do most proteins show cross-species activity?
Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

Pamidronic acid40391-99-9Toll-like Receptor (TLR)RANKL/RANKWntβ-cateninBeta cateninosteoarthritis (OA)osteosarcomaosteoporosisInhibitorinhibitorinhibit

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Pamidronic acid

Customer Review

Other Forms of Pamidronic acid:

Pamidronic acid Related Antibodies

1 Publications Citing Use of MCE Pamidronic acid

Featured Recommendations

•Related Screening Libraries:

•Related Small Molecules:

•Related Proteins:

View All Toll-like Receptor (TLR) Isoform Specific Products:

Biological Activity

Protocol

Purity & Documentation

References

Customer Review

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Dilution Calculator

Complete Stock Solution Preparation Table

Pamidronic acid Related Classifications

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