Procarbazine Hydrochloride

Name: Procarbazine Hydrochloride
Brand: MedChemExpress
SKU: HY-13733
Rating: 5.0 (3 reviews)

Cat. No.: HY-13733 Purity: 99.37%: FAQs
Data Sheet SDS COA Handling Instructions

Molarity Calculator

Procarbazine Hydrochloride is an orally active alkylating agent, with anticancer activity. Procarbazine Hydrochloride can be used in Hodgkin's disease research.

For research use only. We do not sell to patients.

Procarbazine Hydrochloride Chemical Structure

CAS No. : 366-70-1

Size	Price	Stock	Quantity

Free Sample (0.1 - 0.5 mg)		Apply Now
Solid + Solvent (Highly Recommended)
10 mM * 1 mL in DMSO ready for reconstitution	USD 55	In-stock	Estimated Time of Arrival: December 31
Solution
10 mM * 1 mL in DMSO	USD 55	In-stock	Estimated Time of Arrival: December 31
Solid
100 mg	USD 50	In-stock	Estimated Time of Arrival: December 31
500 mg	USD 156	In-stock	Estimated Time of Arrival: December 31
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Based on 3 publication(s) in Google Scholar

Other Forms of Procarbazine Hydrochloride:

Procarbazine Get quote
Procarbazine Hydrochloride (Standard) Get quote

3 Publications Citing Use of MCE Procarbazine Hydrochloride

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Biological Activity
Purity & Documentation
References
Customer Review

Description

Procarbazine Hydrochloride is an orally active alkylating agent, with anticancer activity. Procarbazine Hydrochloride can be used in Hodgkin's disease research^[1]^[2].

In Vitro

Procarbazine Hydrochloride (5 and 20 nM; 1 h) treatment shows cell survival at various concentrarions^[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Cell Viability Assay^[1]

Cell Line:	L1210 cells
Concentration:	5 and 20 nM
Incubation Time:	1 hour
Result:	Showed 99.3% and 99.9% survival of cells at 5 mM and 20 mM, respectively.

In Vivo

Procarbazine (Intraperitoneal injection; 50 and 150 mg/kg; once daily; 5 d) induces micronuclei in hematopoietic cells, but not increases the lacZ mutant frequency (MF) in bone marrow^[2].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model:	Male muta mouse (7–8 weeks old)^[2]
Dosage:	50 and 150 mg/kg
Administration:	Intraperitoneal injection; 50 and 150 mg/kg; once daily; 5 days
Result:	Increased the MN frequency appreciably, and observed micronucleus induction in the peripheral blood at 50 mg/kg.

Clinical Trial

Molecular Weight

257.76

Formula

C₁₂H₂₀ClN₃O

CAS No.

366-70-1

Appearance

Solid

Color

Light yellow to yellow

SMILES

O=C(NC(C)C)C1=CC=C(CNNC)C=C1.Cl

Shipping

Room temperature in continental US; may vary elsewhere.

Storage

4°C, sealed storage, away from moisture

*In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)

Solvent & Solubility

In Vitro:

H₂O : 100 mg/mL (387.96 mM; Need ultrasonic)

DMSO : 16.67 mg/mL (64.67 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Preparing
Stock Solutions

Concentration Solvent Mass	1 mg	5 mg	10 mg

1 mM	3.8796 mL	19.3979 mL	38.7958 mL
5 mM	0.7759 mL	3.8796 mL	7.7592 mL
10 mM	0.3880 mL	1.9398 mL	3.8796 mL

View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

Molarity Calculator
Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

This equation is commonly abbreviated as: C₁V₁ = C₂V₂

Concentration _(start)

Volume _(start)

Concentration _(final)

Volume _(final)

In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

Protocol 1

Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline
Solubility: ≥ 2.08 mg/mL (8.07 mM); Clear solution

This protocol yields a clear solution of ≥ 2.08 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (20.8 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.
Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
Protocol 2

Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in Saline)
Solubility: ≥ 1.67 mg/mL (6.48 mM); Clear solution

This protocol yields a clear solution of ≥ 1.67 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (16.7 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.
Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
Protocol 3

Add each solvent one by one: 10% DMSO 90% Corn Oil
Solubility: ≥ 1.67 mg/mL (6.48 mM); Clear solution

This protocol yields a clear solution of ≥ 1.67 mg/mL (saturation unknown). If the continuous dosing period exceeds half a month, please choose this protocol carefully.
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (16.7 mg/mL) to 900 μL Corn oil, and mix evenly.

For the following dissolution methods, please prepare the working solution directly. It is recommended to prepare fresh solutions and use them promptly within a short period of time.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

Protocol 1

Add each solvent one by one: PBS
Solubility: 100 mg/mL (387.96 mM); Clear solution; Need ultrasonic

In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Calculation results:

Working solution concentration: mg/mL

This product has good water solubility, please refer to the measured solubility data in water/PBS/Saline for details.

The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only.If necessary, please contact MedChemExpress (MCE).

Purity & Documentation

Purity: 99.37%

Select Batch:

Data Sheet (272 KB) SDS (557 KB)

COA (245 KB) HNMR (279 KB) LCMS (264 KB)

Handling Instructions (2659 KB)

References

[1]. J M Erikson, et al. Cytotoxicity and DNA damage caused by the azoxy metabolites of procarbazine in L1210 tumor cells. Cancer Res. 1989 Jan 1;49(1):127-33. [Content Brief]

[2]. T Suzuki, et al. Procarbazine genotoxicity in the MutaMouse; strong clastogenicity and organ-specific induction of lacZ mutations. Mutat Res. 1999 Aug 18;444(2):269-81. [Content Brief]

Complete Stock Solution Preparation Table

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg

DMSO / H₂O	1 mM	3.8796 mL	19.3979 mL	38.7958 mL	96.9894 mL
	5 mM	0.7759 mL	3.8796 mL	7.7592 mL	19.3979 mL
	10 mM	0.3880 mL	1.9398 mL	3.8796 mL	9.6989 mL
	15 mM	0.2586 mL	1.2932 mL	2.5864 mL	6.4660 mL
	20 mM	0.1940 mL	0.9699 mL	1.9398 mL	4.8495 mL
	25 mM	0.1552 mL	0.7759 mL	1.5518 mL	3.8796 mL
	30 mM	0.1293 mL	0.6466 mL	1.2932 mL	3.2330 mL
	40 mM	0.0970 mL	0.4849 mL	0.9699 mL	2.4247 mL
	50 mM	0.0776 mL	0.3880 mL	0.7759 mL	1.9398 mL
	60 mM	0.0647 mL	0.3233 mL	0.6466 mL	1.6165 mL
H₂O	80 mM	0.0485 mL	0.2425 mL	0.4849 mL	1.2124 mL
H₂O	100 mM	0.0388 mL	0.1940 mL	0.3880 mL	0.9699 mL

* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.