Promazine hydrochloride (Synonyms: Romtiazin hydrochloride; Ampazine hydrochloride; Berophen hydrochloride)

Name: Promazine hydrochloride
Brand: MedChemExpress
SKU: HY-B1225
Rating: 4.5 (1 reviews)

Cat. No.: HY-B1225 Purity: 99.87%: FAQs
Data Sheet SDS COA Handling Instructions

Molarity Calculator

Promazine (Romtiazin) hydrochloride is an antipsychotic and a dopamine receptor D₂ antagonist. Promazine hydrochloride inhibits dopaminergic neurotransmission.

For research use only. We do not sell to patients.

Promazine hydrochloride Chemical Structure

CAS No. : 53-60-1

Size	Price	Stock	Quantity
Solid + Solvent (Highly Recommended)
10 mM * 1 mL in DMSO ready for reconstitution	USD 33	In-stock	Estimated Time of Arrival: December 31
Solution
10 mM * 1 mL in DMSO	USD 33	In-stock	Estimated Time of Arrival: December 31
Solid
100 mg	USD 30	In-stock	Estimated Time of Arrival: December 31
500 mg	USD 50	In-stock	Estimated Time of Arrival: December 31
1 g		Get quote
5 g		Get quote

or Bulk Inquiry

* Please select Quantity before adding items.

This product is a controlled substance and not for sale in your territory.

Customer Review

Based on 1 publication(s) in Google Scholar

Other Forms of Promazine hydrochloride:

Promazine Get quote

Featured Recommendations

•Related Small Molecules:

•Related Proteins:

View All Dopamine Receptor Isoform Specific Products:

View All Isoforms

Dopamine Receptor D₁ Receptor D₂ Receptor D₃ Receptor D₄ Receptor D₅ Receptor

Biological Activity
Purity & Documentation
References
Customer Review

Description

Promazine (Romtiazin) hydrochloride is an antipsychotic and a dopamine receptor D₂ antagonist. Promazine hydrochloride inhibits dopaminergic neurotransmission^[1]^[2].

IC₅₀ & Target^[1]

D₂ Receptor

Cellular Effect

Cell Line	Type	Value	Description	References
HEK293	IC₅₀	17.2 μM Compound: promazine	Inhibition of 4-(4-(dimethylamino)styryl)-N-methylpyridinium uptake at human OCT1 expressed in HEK293 cells by confocal microscopy Inhibition of 4-(4-(dimethylamino)styryl)-N-methylpyridinium uptake at human OCT1 expressed in HEK293 cells by confocal microscopy	[PMID: 18788725]

In Vitro

Promazine hydrochloride shows K_Ds of 25, 190 and 8400 nM at the human norepinephrine transporter, serotonin transporter, and dopamine transporter, respectively^[3].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Molecular Weight

320.88

Formula

C₁₇H₂₁ClN₂S

CAS No.

53-60-1

Appearance

Solid

Color

White to off-white

SMILES

CN(C)CCCN1C2=C(C=CC=C2)SC3=CC=CC=C13.[H]Cl

Shipping

Room temperature in continental US; may vary elsewhere.

Storage

4°C, sealed storage, away from moisture

*In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)

Solvent & Solubility

In Vitro:

H₂O : 100 mg/mL (311.64 mM; Need ultrasonic)

DMSO : 100 mg/mL (311.64 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Preparing
Stock Solutions

Concentration Solvent Mass	1 mg	5 mg	10 mg

1 mM	3.1164 mL	15.5821 mL	31.1643 mL
5 mM	0.6233 mL	3.1164 mL	6.2329 mL
10 mM	0.3116 mL	1.5582 mL	3.1164 mL

View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

Molarity Calculator
Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

This equation is commonly abbreviated as: C₁V₁ = C₂V₂

Concentration _(start)

Volume _(start)

Concentration _(final)

Volume _(final)

In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

Protocol 1

Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline
Solubility: ≥ 2.5 mg/mL (7.79 mM); Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.
Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
Protocol 2

Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in Saline)
Solubility: ≥ 2.5 mg/mL (7.79 mM); Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.
Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
Protocol 3

Add each solvent one by one: 10% DMSO 90% Corn Oil
Solubility: ≥ 2.5 mg/mL (7.79 mM); Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown). If the continuous dosing period exceeds half a month, please choose this protocol carefully.
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL Corn oil, and mix evenly.

For the following dissolution methods, please prepare the working solution directly. It is recommended to prepare fresh solutions and use them promptly within a short period of time.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

Protocol 1

Add each solvent one by one: PBS
Solubility: 100 mg/mL (311.64 mM); Clear solution; Need ultrasonic

In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Calculation results:

Working solution concentration: mg/mL

This product has good water solubility, please refer to the measured solubility data in water/PBS/Saline for details.

The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only.If necessary, please contact MedChemExpress (MCE).

Purity & Documentation

Purity: 99.87%

Select Batch:

Data Sheet (273 KB) SDS (393 KB)

COA (250 KB) LCMS (93 KB)

Handling Instructions (2659 KB)

References

[1]. Gareri P, et al. Conventional and atypical antipsychotics in the elderly : a review. Clin Drug Investig. 2003;23(5):287-322. [Content Brief]

[2]. Myers PR, et al. Characterization of a depolarizing dopamine response in a vertebrate neuronal somatic cell hybrid. J Cell Physiol. 1977;91(1):103-118. [Content Brief]

[3]. Tatsumi M, et al. Pharmacological profile of neuroleptics at human monoamine transporters. Eur J Pharmacol. 1999;368(2-3):277-283. [Content Brief]

Complete Stock Solution Preparation Table

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg

H₂O / DMSO	1 mM	3.1164 mL	15.5821 mL	31.1643 mL	77.9107 mL
	5 mM	0.6233 mL	3.1164 mL	6.2329 mL	15.5821 mL
	10 mM	0.3116 mL	1.5582 mL	3.1164 mL	7.7911 mL
	15 mM	0.2078 mL	1.0388 mL	2.0776 mL	5.1940 mL
	20 mM	0.1558 mL	0.7791 mL	1.5582 mL	3.8955 mL
	25 mM	0.1247 mL	0.6233 mL	1.2466 mL	3.1164 mL
	30 mM	0.1039 mL	0.5194 mL	1.0388 mL	2.5970 mL
	40 mM	0.0779 mL	0.3896 mL	0.7791 mL	1.9478 mL
	50 mM	0.0623 mL	0.3116 mL	0.6233 mL	1.5582 mL
	60 mM	0.0519 mL	0.2597 mL	0.5194 mL	1.2985 mL
	80 mM	0.0390 mL	0.1948 mL	0.3896 mL	0.9739 mL
	100 mM	0.0312 mL	0.1558 mL	0.3116 mL	0.7791 mL

* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

Promazine hydrochloride Related Classifications

Neurological Disease

Help & FAQs

Do most proteins show cross-species activity?
Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

Promazine53-60-1RomtiazinAmpazineBerophenDopamine ReceptornorepinephrinetransporterantipsychoticphenothiazinInhibitorinhibitorinhibit

Your Recently Viewed Products:

Product Name

Salutation

Applicant Name *

Email Address *

Phone Number *

Organization Name *

Department *

Requested quantity *

Country or Region *

Remarks

Product Name

Lot #

Applicant Name *

Email Address *

Phone Number

Department *

Organization Name *

Country or Region *

Remarks

Name
Email *

	Sorry, but the email address you supplied was invalid.

Promazine hydrochloride (Synonyms: Romtiazin hydrochloride; Ampazine hydrochloride; Berophen hydrochloride)

Customer Review

Other Forms of Promazine hydrochloride:

Promazine hydrochloride Related Antibodies

Featured Recommendations

•Related Small Molecules:

•Related Proteins:

View All Dopamine Receptor Isoform Specific Products:

Biological Activity

Purity & Documentation

References

Customer Review

Promazine hydrochloride Related Antibodies

Molarity Calculator

Dilution Calculator

Complete Stock Solution Preparation Table

Promazine hydrochloride Related Classifications

Keywords:

Your Recently Viewed Products:

Customer Review

Request for HNMR Report

SAFETY DATA SHEET (SDS)

Request for HNMR Report