2. Autophagy
  3. Autophagy
  4. QX77

QX77 is a chaperone-mediated autophagy (CMA) activator and upregulates LAMP2A expression in vitro. QX77 induces Rab11 upregulation, rescues Rab11 down-regulation and trafficking deficiency in cystinotic cells. QX77 can impede self-renewal and promote differentiation of ES cells.

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QX77 Chemical Structure

QX77 Chemical Structure

CAS No. : 1798331-92-6

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Based on 3 publication(s) in Google Scholar

QX77 Related Antibodies

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Description

QX77 is a chaperone-mediated autophagy (CMA) activator and upregulates LAMP2A expression in vitro. QX77 induces Rab11 upregulation, rescues Rab11 down-regulation and trafficking deficiency in cystinotic cells[1]. QX77 can impede self-renewal and promote differentiation of ES cells[2].

IC50 & Target

CMA[1]
In Vitro

QX77 (48 hours) upregulates Rab11 expression levels in Ctns-/-?MEFs[1].
? In Ctns-/- MEFs, QX77 recovers the down-regulated LAMP2A expression, and the Rab11-positive carrier vesicles recover the high-motility trafficking phenotype observe in wild-type cells[1].
Treatment with CMA activator QX77 rescues Rab11 down-regulation and trafficking deficiency in cystinotic cells. QX77 treatment also increases LAMP2A localization at the lysosomal membrane[1].
QX77 significantly increases the re-localization of LAMP2A at LAMP1-positive lysosomes in cystinotic cell, it corrects the localization of LAMP2A at the lysosomal membrane in cystinotic cells[1].
? QX77 protects cystinotic cells from the increased susceptibility to? tert-butyl-hydroperoxide-induced oxidative stress and reconstitutes the resistant levels observed in wild-type cells. The effect of QX77 on cystinotic cell survival is dependent on LAMP2A expression[1].
QX77 (10 μM;? 0, 3 or 6 days) activates CMA and increases LAMP2A expression in? D3 and E14 ES cell, it downregulates pluripotency factors and AP reactivity and partially lost the characteristic ES cell morphology[2].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

QX77 Related Antibodies

Western Blot Analysis[2]

Cell Line: ES D3 cell line; E14TG2a (E14) cell line
Concentration: 10 μM
Incubation Time: 3 or 6 days
Result: Increased LAMP2A expression and decreased SOX2 and Oct4 protein expression.
Molecular Weight

300.74

Formula

C16H13ClN2O2
CAS No.
Appearance

Solid

Color

Light yellow to yellow

SMILES

CC(NC1=CC=C(C2=NC3=CC=C(Cl)C=C3OC2)C=C1)=O
Shipping

Room temperature in continental US; may vary elsewhere.
Storage
Powder -20°C 3 years
4°C 2 years
In solvent -80°C 6 months
-20°C 1 month
Solvent & Solubility
In Vitro: 

DMSO : 15 mg/mL (49.88 mM; ultrasonic and warming and heat to 60°C; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Preparing
Stock Solutions
Concentration Solvent Mass 1 mg 5 mg 10 mg
1 mM 3.3251 mL 16.6257 mL 33.2513 mL
5 mM 0.6650 mL 3.3251 mL 6.6503 mL
View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

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In Vivo:

For the following dissolution methods, please prepare the working solution directly. It is recommended to prepare fresh solutions and use them promptly within a short period of time.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

  • Protocol 1

    Add each solvent one by one:  50% PEG300    50% Saline

    Solubility: 10 mg/mL (33.25 mM); Suspended solution; Need ultrasonic

In Vivo Dissolution Calculator
Please enter the basic information of animal experiments:

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Calculation results:
Working solution concentration: mg/mL
Purity & Documentation

Purity: 99.48%

SDS (393 KB)

COA (245 KB) HNMR (255 KB) LCMS (266 KB)

Handling Instructions (2659 KB)
References

Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
DMSO 1 mM 3.3251 mL 16.6257 mL 33.2513 mL 83.1283 mL
5 mM 0.6650 mL 3.3251 mL 6.6503 mL 16.6257 mL
10 mM 0.3325 mL 1.6626 mL 3.3251 mL 8.3128 mL
15 mM 0.2217 mL 1.1084 mL 2.2168 mL 5.5419 mL
20 mM 0.1663 mL 0.8313 mL 1.6626 mL 4.1564 mL
25 mM 0.1330 mL 0.6650 mL 1.3301 mL 3.3251 mL
30 mM 0.1108 mL 0.5542 mL 1.1084 mL 2.7709 mL
40 mM 0.0831 mL 0.4156 mL 0.8313 mL 2.0782 mL
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QX77 Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

QX771798331-92-6QX 77QX-77AutophagyRab11MEFsLAMP2ACMApluripotencyESInhibitorinhibitorinhibit

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