Thiomyristoyl

Name: Thiomyristoyl
Brand: MedChemExpress
SKU: HY-101278
Rating: 5.0 (8 reviews)

Cat. No.: HY-101278 Purity: 99.74%: FAQs
Data Sheet SDS COA Handling Instructions

Molarity Calculator

Thiomyristoyl is a potent and specific SIRT2 inhibitor with an IC₅₀ of 28 nM.

For research use only. We do not sell to patients.

Thiomyristoyl Chemical Structure

CAS No. : 1429749-41-6

Size	Price	Stock	Quantity
Solid + Solvent (Highly Recommended)
10 mM * 1 mL in DMSO ready for reconstitution	USD 155	In-stock	Estimated Time of Arrival: December 31
Solution
10 mM * 1 mL in DMSO	USD 155	In-stock	Estimated Time of Arrival: December 31
Solid
1 mg	USD 66	In-stock	Estimated Time of Arrival: December 31
5 mg	USD 121	In-stock	Estimated Time of Arrival: December 31
10 mg	USD 165	In-stock	Estimated Time of Arrival: December 31
25 mg	USD 319	In-stock	Estimated Time of Arrival: December 31
50 mg	USD 539	In-stock	Estimated Time of Arrival: December 31
100 mg	USD 825	In-stock	Estimated Time of Arrival: December 31
200 mg		Get quote
500 mg		Get quote

or Bulk Inquiry

* Please select Quantity before adding items.

This product is a controlled substance and not for sale in your territory.

Customer Review

Based on 8 publication(s) in Google Scholar

5 Publications Citing Use of MCE Thiomyristoyl

: Thiomyristoyl purchased from MedChemExpress. Usage Cited in: Neoplasia. 2019 Mar 29;21(5):429-441. [Abstract]; After treatment with Tm (Thiomyristoyl), NAC, or a combination of both, the levels of ROS and proteins are detected.

: Thiomyristoyl purchased from MedChemExpress. Usage Cited in: Neoplasia. 2019 Mar 29;21(5):429-441. [Abstract]; cMYC, p-PDHA1, PHGDH, PSAT1 and PSPH protein levels in xenograft tissues from control and Tm (Thiomyristoyl) group are detected by staining and Western blotting.

: Thiomyristoyl purchased from MedChemExpress. Usage Cited in: Neoplasia. 2019 Mar 29;21(5):429-441. [Abstract]; HuCCT1 and RBE cells are treated with 0, 25, or 50 μm Tm (Thiomyristoyl) and subjected to western blotting

Featured Recommendations

•Related Screening Libraries:

•Related Small Molecules:

•Related Proteins:

View All Sirtuin Isoform Specific Products:

View All Isoforms

Sirtuin SIRT1 SIRT2 SIRT3 SIRT6 SIRT5 SIRT7

Biological Activity
Protocol
Purity & Documentation
References
Customer Review

Description

Thiomyristoyl is a potent and specific SIRT2 inhibitor with an IC₅₀ of 28 nM.

IC₅₀ & Target^[1]

SIRT2

28 nM (IC₅₀)

SIRT1

98 μM (IC₅₀)

Cellular Effect

Cell Line	Type	Value	Description	References
A549	GI₅₀	17.3 μM Compound: TM	Growth inhibition of human A549 cells after 72 hrs by CellTiter-Blue assay Growth inhibition of human A549 cells after 72 hrs by CellTiter-Blue assay	[PMID: 30986062]
BXPC-3	GI₅₀	13.3 μM Compound: TM	Growth inhibition of human BxPC3 cells after 72 hrs by CellTiter-Blue assay Growth inhibition of human BxPC3 cells after 72 hrs by CellTiter-Blue assay	[PMID: 30986062]
HCT-116	GI₅₀	131.6 μM Compound: TM	Growth inhibition of human HCT116 cells after 72 hrs by CellTiter-Blue assay Growth inhibition of human HCT116 cells after 72 hrs by CellTiter-Blue assay	[PMID: 30986062]
HCT-116	GI₅₀	16.7 μM Compound: TM	Inhibition of anchorage-independent cell growth in human HCT116 cells assessed as reduction in colony formation incubated for 5 days followed by compound re-addition and measured after 9 to 11 days by nitro blue tetrazolium chloride-based soft agar colony Inhibition of anchorage-independent cell growth in human HCT116 cells assessed as reduction in colony formation incubated for 5 days followed by compound re-addition and measured after 9 to 11 days by nitro blue tetrazolium chloride-based soft agar colony	[PMID: 30986062]
MCF7	GI₅₀	37 μM Compound: TM	Growth inhibition of human MCF7 cells after 72 hrs by CellTiter-Blue assay Growth inhibition of human MCF7 cells after 72 hrs by CellTiter-Blue assay	[PMID: 30986062]
MDA-MB-231	GI₅₀	42.8 μM Compound: TM	Growth inhibition of human MDA-MB-231 cells after 72 hrs by CellTiter-Blue assay Growth inhibition of human MDA-MB-231 cells after 72 hrs by CellTiter-Blue assay	[PMID: 30986062]
MDA-MB-468	GI₅₀	15.7 μM Compound: TM	Growth inhibition of human MDA-MB-468 cells after 72 hrs by CellTiter-Blue assay Growth inhibition of human MDA-MB-468 cells after 72 hrs by CellTiter-Blue assay	[PMID: 30986062]
NCI-H23	GI₅₀	16.4 μM Compound: TM	Growth inhibition of human NCI-H23 cells after 72 hrs by CellTiter-Blue assay Growth inhibition of human NCI-H23 cells after 72 hrs by CellTiter-Blue assay	[PMID: 30986062]
SW948	GI₅₀	19.2 μM Compound: TM	Growth inhibition of human SW948 cells after 72 hrs by CellTiter-Blue assay Growth inhibition of human SW948 cells after 72 hrs by CellTiter-Blue assay	[PMID: 30986062]

In Vitro

Thiomyristoyl (TM) is a potent SIRT2-specific inhibitor with broad anticancer activity but little effect on non-cancerous cells. SIRT2-inhibition promotes c-Myc ubiquitination and degradation, suggesting the therapeutic potential of TM to target certain c-Myc-driven cancers. TM could inhibit SIRT2 with an IC₅₀ of 28 nM, but inhibits SIRT1 with an IC₅₀ value of 98 μM and does not inhibit SIRT3 even at 200 μM. TM inhibits three human breast cancer cell lines, MCF-7, MDA-MB-468, and MDA-MB-231^[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

In Vivo

TM inhibits tumor growth in mouse models of breast cancer. TM does not cause significant toxicity in mice and no significant weight loss is observed in TM-treated mice. S5H, the acetyl-a-tubulin level is moderately but statistically significantly increased in tumors from TM-treated mice compared with those from vehicle-treated mice, suggesting that TM indeed inhibits SIRT2 in vivo^[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Molecular Weight

581.85

Formula

C₃₄H₅₁N₃O₃S

CAS No.

1429749-41-6

Appearance

Solid

Color

White to off-white

SMILES

O=C(OCC1=CC=CC=C1)N[C@H](C(NC2=CC=CC=C2)=O)CCCCNC(CCCCCCCCCCCCC)=S

Shipping

Room temperature in continental US; may vary elsewhere.

Storage

Powder	-20°C	3 years
	4°C	2 years
In solvent	-80°C	2 years
	-20°C	1 year

Solvent & Solubility

In Vitro:

DMSO : ≥ 32 mg/mL (55.00 mM; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Ethanol : 15.29 mg/mL (26.28 mM; Need ultrasonic)

*"≥" means soluble, but saturation unknown.

Preparing
Stock Solutions

Concentration Solvent Mass	1 mg	5 mg	10 mg

1 mM	1.7187 mL	8.5933 mL	17.1866 mL
5 mM	0.3437 mL	1.7187 mL	3.4373 mL
10 mM	0.1719 mL	0.8593 mL	1.7187 mL

View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 2 years; -20°C, 1 year. When stored at -80°C, please use it within 2 years. When stored at -20°C, please use it within 1 year.

Molarity Calculator
Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

This equation is commonly abbreviated as: C₁V₁ = C₂V₂

Concentration _(start)

Volume _(start)

Concentration _(final)

Volume _(final)

In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

Protocol 1

Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline
Solubility: ≥ 2.5 mg/mL (4.30 mM); Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.
Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
Protocol 2

Add each solvent one by one: 10% DMSO 90% Corn Oil
Solubility: ≥ 2.5 mg/mL (4.30 mM); Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown). If the continuous dosing period exceeds half a month, please choose this protocol carefully.
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL Corn oil, and mix evenly.

In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Please enter your animal formula composition:

DMSO +

Tween-80 +

Saline

Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.

The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).

Calculation results:

Working solution concentration: mg/mL

Method for preparing stock solution: mg drug dissolved in μL DMSO (Stock solution concentration: mg/mL).

The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only. If necessary, please contact MedChemExpress (MCE).

Method for preparing in vivo working solution for animal experiments: Take μL DMSO stock solution, add μL . μL , mix evenly, next add μL Tween 80, mix evenly, then add μL Saline.

If the continuous dosing period exceeds half a month, please choose this protocol carefully.

Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.

Purity & Documentation

Purity: 99.74%

Select Batch:

Data Sheet (270 KB) SDS (701 KB)

COA (241 KB) RP-HPLC (210 KB) LCMS (94 KB)

Handling Instructions (2659 KB)

References

[1]. Jing H, et al. A SIRT2-Selective Inhibitor Promotes c-Myc Oncoprotein Degradation and Exhibits Broad Anticancer Activity. Cancer Cell. 2016 Mar 14;29(3):297-310. [Content Brief]

Cell Assay
^[1]

Cells are seeded into 96-well plates at 3,000–4,000 cells per well. After 24 hr, test compounds (Thiomyristoyl) are added to cells to final concentrations ranging from 1 to 50 μM. Cells are then incubated for 72 hr and cell viability is measured using the CellTiter-Blue viability assay. Relative cell viability in the presence of test compounds is normalized to the vehicle-treated controls after background subtraction. GraphPad Prism software is used to determine the IC₅₀ values. Knockdown of SIRT1-7 in various cell lines is achieved by lentiviral infection^[1].

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

References

[1]. Jing H, et al. A SIRT2-Selective Inhibitor Promotes c-Myc Oncoprotein Degradation and Exhibits Broad Anticancer Activity. Cancer Cell. 2016 Mar 14;29(3):297-310. [Content Brief]

Complete Stock Solution Preparation Table

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg

Ethanol / DMSO	1 mM	1.7187 mL	8.5933 mL	17.1866 mL	42.9664 mL
	5 mM	0.3437 mL	1.7187 mL	3.4373 mL	8.5933 mL
	10 mM	0.1719 mL	0.8593 mL	1.7187 mL	4.2966 mL
	15 mM	0.1146 mL	0.5729 mL	1.1458 mL	2.8644 mL
	20 mM	0.0859 mL	0.4297 mL	0.8593 mL	2.1483 mL
	25 mM	0.0687 mL	0.3437 mL	0.6875 mL	1.7187 mL
DMSO	30 mM	0.0573 mL	0.2864 mL	0.5729 mL	1.4322 mL
	40 mM	0.0430 mL	0.2148 mL	0.4297 mL	1.0742 mL
	50 mM	0.0344 mL	0.1719 mL	0.3437 mL	0.8593 mL

Thiomyristoyl Related Classifications

Help & FAQs

Do most proteins show cross-species activity?
Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

Thiomyristoyl1429749-41-6SirtuinInhibitorinhibitorinhibit

Your Recently Viewed Products:

Product Name

Salutation

Applicant Name *

Email Address *

Phone Number *

Organization Name *

Department *

Requested quantity *

Country or Region *

Remarks

Product Name

Lot #

Applicant Name *

Email Address *

Phone Number

Department *

Organization Name *

Country or Region *

Remarks

Name
Email *

	Sorry, but the email address you supplied was invalid.

Thiomyristoyl

Customer Review

Thiomyristoyl Related Antibodies

5 Publications Citing Use of MCE Thiomyristoyl

Featured Recommendations

•Related Screening Libraries:

•Related Small Molecules:

•Related Proteins:

View All Sirtuin Isoform Specific Products:

Biological Activity

Protocol

Purity & Documentation

References

Customer Review

Thiomyristoyl Related Antibodies

Molarity Calculator

Dilution Calculator

Complete Stock Solution Preparation Table

Thiomyristoyl Related Classifications

Keywords:

Your Recently Viewed Products:

Customer Review

Request for HNMR Report

SAFETY DATA SHEET (SDS)

Request for HNMR Report