Thymidine (Synonyms: DThyd; NSC 21548)

Name: Thymidine
Brand: MedChemExpress
SKU: HY-N1150
Rating: 5.0 (8 reviews)

Cat. No.: HY-N1150 Purity: 99.96%: FAQs
Data Sheet SDS COA Handling Instructions

Molarity Calculator

Thymidine, a specific precursor of deoxyribonucleic acid, is used as a cell synchronizing agent. Thymidine is a DNA synthesis inhibitor that can arrest cell at G1/S boundary, prior to DNA replication.

For research use only. We do not sell to patients.

Thymidine Chemical Structure

CAS No. : 50-89-5

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Free Sample (0.1 - 0.5 mg)		Apply Now
500 mg	USD 55	In-stock	Estimated Time of Arrival: December 31
1 g	USD 66	In-stock	Estimated Time of Arrival: December 31
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10 g		Get quote

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Customer Review

Based on 8 publication(s) in Google Scholar

Other Forms of Thymidine:

8 Publications Citing Use of MCE Thymidine

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•Related Screening Libraries:

•Related Small Molecules:

•Related Proteins:

Biological Activity
Purity & Documentation
References
Customer Review

Description

IC₅₀ & Target^[2]

Microbial Metabolite

DNA Synthesis

Human Endogenous Metabolite

Cellular Effect

Cell Line	Type	Value	Description	References
CCRF-CEM	CC₅₀	10.6 μM Compound: 1i	Cytotoxicity in human CEM/0 cells assessed as reduction in cell viability incubated for 4 to 5 days Cytotoxicity in human CEM/0 cells assessed as reduction in cell viability incubated for 4 to 5 days	[PMID: 32515595]
HeLa	IC₅₀	4000 μM Compound: 1	Inhibitory concentration against HeLa cell proliferation after 72 hours of incubation Inhibitory concentration against HeLa cell proliferation after 72 hours of incubation	[PMID: 15582414]
HepG2	CC₅₀	2100 μM Compound: beta-L-dT	Cytotoxicity against human HepG2 cells after 3 days by MTT assay Cytotoxicity against human HepG2 cells after 3 days by MTT assay	[PMID: 17404006]
HepG2 2.2.15	EC₅₀	0.3 μM Compound: beta-L-dT	Antiviral activity against HBV in HepG2.2.15 cells assessed as decrease in extracellular viral DNA level by RT-PCR Antiviral activity against HBV in HepG2.2.15 cells assessed as decrease in extracellular viral DNA level by RT-PCR	[PMID: 17404006]
HL-60	CC₅₀	1760 μM Compound: beta-L-dT	Cytotoxicity against human HL60 cells after 3 days by MTT assay Cytotoxicity against human HL60 cells after 3 days by MTT assay	[PMID: 17404006]
Vero	IC₅₀	0.3 nM Compound: Thymidine	Anti-Herpes simplex virus type-1 activity in vero cells using plaque inhibition assay Anti-Herpes simplex virus type-1 activity in vero cells using plaque inhibition assay	[PMID: 10464017]
Vero	IC₅₀	0.4 nM Compound: thymidine	Anti-Herpes simplex virus type-1 activity in vero cells using plaque inhibition assay Anti-Herpes simplex virus type-1 activity in vero cells using plaque inhibition assay	[PMID: 10464017]

In Vivo

Thymidine (500 mg/kg; i.p.; twice a day) completely reverses both Methotrexate- and Tomudex-induced deletion of both CD4⁺Vβ8⁺ and CD8⁺Vβ8⁺ T cells ^[3].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model:	8-12 weeks BALB/c mice^[3]
Dosage:	500 mg/kg
Administration:	i.p.; twice a day
Result:	Completely abrogates Methotrexate- and Tomudex-induced deletion of Vβ8⁺ T cells after SEB injection.

Clinical Trial

Molecular Weight

242.23

Formula

C₁₀H₁₄N₂O₅

CAS No.

50-89-5

Appearance

Solid

Color

White to off-white

SMILES

O[C@H]1C[C@H](N2C(NC(C(C)=C2)=O)=O)O[C@@H]1CO

Structure Classification

Others

Initial Source

Microorganisms

Shipping

Room temperature in continental US; may vary elsewhere.

Storage

Powder	-20°C	3 years
	4°C	2 years
In solvent	-80°C	6 months
	-20°C	1 month

Solvent & Solubility

In Vitro:

DMSO : 50 mg/mL (206.42 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

H₂O : 33.33 mg/mL (137.60 mM; Need ultrasonic)

Preparing
Stock Solutions

Concentration Solvent Mass	1 mg	5 mg	10 mg

1 mM	4.1283 mL	20.6415 mL	41.2831 mL
5 mM	0.8257 mL	4.1283 mL	8.2566 mL
10 mM	0.4128 mL	2.0642 mL	4.1283 mL

View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

Molarity Calculator
Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

This equation is commonly abbreviated as: C₁V₁ = C₂V₂

Concentration _(start)

Volume _(start)

Concentration _(final)

Volume _(final)

In Vivo:

For the following dissolution methods, please prepare the working solution directly. It is recommended to prepare fresh solutions and use them promptly within a short period of time.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

Protocol 1

Add each solvent one by one: PBS
Solubility: 20 mg/mL (82.57 mM); Clear solution; Need ultrasonic and warming and heat to 60°C

In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Calculation results:

Working solution concentration: mg/mL

This product has good water solubility, please refer to the measured solubility data in water/PBS/Saline for details.

The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only.If necessary, please contact MedChemExpress (MCE).

Purity & Documentation

Purity: 99.96%

Select Batch:

Data Sheet (278 KB) SDS (393 KB)

COA (252 KB) RP-HPLC (243 KB)

Handling Instructions (2659 KB)

References

[1]. Chen G, et al. Cell Synchronization by Double Thymidine Block. Bio Protoc. 2018 Sep 5;8(17). [Content Brief]

[2]. FIRKET H, et al. Autoradiographic visualization of synthesis of deoxyribonucleic acid in tissue culture with tritium-labelled thymidine. Nature. 1958 Jan 24;181(4604):274-5.FIRKET H, et al. Autoradiographic visualization of synthesis of deoxyribonucleic acid in tissue culture with tritium-labelled thymidine. Nature. 1958 Jan 24;181(4604):274-5. [Content Brief]

[3]. Izeradjene K, et al. Inhibition of thymidine synthesis by folate analogues induces a Fas-Fas ligand-independentdeletion of superantigen-reactive peripheral T cells. Int Immunol. 2001 Jan;13(1):85-93. [Content Brief]

Complete Stock Solution Preparation Table

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg

H₂O / DMSO	1 mM	4.1283 mL	20.6415 mL	41.2831 mL	103.2077 mL
	5 mM	0.8257 mL	4.1283 mL	8.2566 mL	20.6415 mL
	10 mM	0.4128 mL	2.0642 mL	4.1283 mL	10.3208 mL
	15 mM	0.2752 mL	1.3761 mL	2.7522 mL	6.8805 mL
	20 mM	0.2064 mL	1.0321 mL	2.0642 mL	5.1604 mL
	25 mM	0.1651 mL	0.8257 mL	1.6513 mL	4.1283 mL
	30 mM	0.1376 mL	0.6881 mL	1.3761 mL	3.4403 mL
	40 mM	0.1032 mL	0.5160 mL	1.0321 mL	2.5802 mL
	50 mM	0.0826 mL	0.4128 mL	0.8257 mL	2.0642 mL
	60 mM	0.0688 mL	0.3440 mL	0.6881 mL	1.7201 mL
	80 mM	0.0516 mL	0.2580 mL	0.5160 mL	1.2901 mL
	100 mM	0.0413 mL	0.2064 mL	0.4128 mL	1.0321 mL

* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

Thymidine Related Classifications

Help & FAQs

Do most proteins show cross-species activity?
Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

Thymidine50-89-5DThyd NSC 21548NSC21548NSC 21548NSC-21548DNA/RNA SynthesisEndogenous MetaboliteOrthopoxvirusprecursorsynchronizingarrestDNAreplicationInhibitorinhibitorinhibit

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Thymidine (Synonyms: DThyd; NSC 21548)

Customer Review

Other Forms of Thymidine:

Thymidine Related Antibodies

8 Publications Citing Use of MCE Thymidine

Featured Recommendations

•Related Screening Libraries:

•Related Small Molecules:

•Related Proteins:

Biological Activity

Purity & Documentation

References

Customer Review

Molarity Calculator

Dilution Calculator

Complete Stock Solution Preparation Table

Thymidine Related Classifications

Keywords:

Your Recently Viewed Products:

Customer Review

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SAFETY DATA SHEET (SDS)

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