Tiamulin fumarate (Synonyms: Thiamutilin fumarate)

Cat. No.: HY-B2060A Purity: ≥98.0%: FAQs
Data Sheet SDS COA Handling Instructions

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Tiamulin (Thiamutilin) fumarate is a diterpenic antibiotic that is widely used in pigs and poultry for the control of infectious diseases. Tiamulin fumarate is effectively used in the study of airsacculitis, which is primarily caused by Mycoplasma spp.

For research use only. We do not sell to patients.

Tiamulin fumarate Chemical Structure

CAS No. : 55297-96-6

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Solid + Solvent (Highly Recommended)
10 mM * 1 mL in DMSO ready for reconstitution	USD 73	In-stock	Estimated Time of Arrival: December 31
Solution
10 mM * 1 mL in DMSO	USD 73	In-stock	Estimated Time of Arrival: December 31
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250 mg	USD 66	In-stock	Estimated Time of Arrival: December 31
1 g	USD 132	In-stock	Estimated Time of Arrival: December 31
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Other Forms of Tiamulin fumarate:

Tiamulin Get quote
Tiamulin fumarate (Standard) Get quote

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Biological Activity
Purity & Documentation
References
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Description

In Vitro

Tiamulin fumarate is a semisynthetic derivative of the diterpene antibiotic Pleuromutilin and is effectively used in the study of airsacculitis, which is primarily caused by Mycoplasma spp^[1].
Tiamulin fumarate is highly active in vitro against Mycoplasma strains (M. gallisepticum, M. synoviae, M. meleagridis, and M. iowae), Spirochaetes (Brachyspira hyodysenteriae, Brachyspira innocens, B. pilosicoli, B. intermedia), gram-positive bacteria (staphylococci, streptococci, Clostridia, Arcanobacterium spp), but less active against gram-negative bacteria (Pasteurella, Klebsiella, Haemophilus, Fusobacterium, Campylobacter, Bacteroides spp.)^[1].
Tiamulin fumarate binds with the rRNA in the peptidyl transferase slot on the ribosome, in which it prevents the correct positioning of the CCA ends of tRNA for peptide transferase and subsequent protein production^[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

In Vivo

Tiamulin fumarate is highly effective in the treatment of avian intestinal spirochaetosis in breeder and layer hens at 25 mg/kg of BW per day over 5 d in artificial infection studies with B. pilosicoli and B. intermedia, respectively^[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Molecular Weight

609.81

Formula

C₃₂H₅₁NO₈S

CAS No.

55297-96-6

Appearance

Solid

Color

White to off-white

SMILES

O=C(O[C@H]1[C@]([C@H](C)CC2)(C)[C@@](C(CC3)=O)([H])[C@]32[C@@H](C)[C@H](O)[C@](C)(C=C)C1)CSCCN(CC)CC.O=C(O)/C=C/C(O)=O

Shipping

Room temperature in continental US; may vary elsewhere.

Storage

4°C, sealed storage, away from moisture

*In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)

Solvent & Solubility

In Vitro:

DMSO : ≥ 100 mg/mL (163.99 mM; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

H₂O : 100 mg/mL (163.99 mM; Need ultrasonic)

*"≥" means soluble, but saturation unknown.

Preparing
Stock Solutions

Concentration Solvent Mass	1 mg	5 mg	10 mg

1 mM	1.6399 mL	8.1993 mL	16.3986 mL
5 mM	0.3280 mL	1.6399 mL	3.2797 mL
10 mM	0.1640 mL	0.8199 mL	1.6399 mL

View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

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Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

This equation is commonly abbreviated as: C₁V₁ = C₂V₂

Concentration _(start)

Volume _(start)

Concentration _(final)

Volume _(final)

In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

Protocol 1

Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline
Solubility: ≥ 2.5 mg/mL (4.10 mM); Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.
Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
Protocol 2

Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in Saline)
Solubility: ≥ 2.5 mg/mL (4.10 mM); Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.
Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
Protocol 3

Add each solvent one by one: 10% DMSO 90% Corn Oil
Solubility: ≥ 2.5 mg/mL (4.10 mM); Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown). If the continuous dosing period exceeds half a month, please choose this protocol carefully.
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL Corn oil, and mix evenly.

For the following dissolution methods, please prepare the working solution directly. It is recommended to prepare fresh solutions and use them promptly within a short period of time.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

Protocol 1

Add each solvent one by one: PBS
Solubility: 100 mg/mL (163.99 mM); Clear solution; Need ultrasonic

In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Calculation results:

Working solution concentration: mg/mL

This product has good water solubility, please refer to the measured solubility data in water/PBS/Saline for details.

The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only.If necessary, please contact MedChemExpress (MCE).

Purity & Documentation

Purity: ≥98.0%

Select Batch:

Data Sheet (278 KB) SDS (394 KB)

COA (258 KB) HNMR (283 KB)

Handling Instructions (2659 KB)

References

[1]. Dreyfuss J, et al. Metabolism of tritium- and carbon-14-labeled tiamulin in dogs, rats, and pigs. J Antibiot (Tokyo). 1979 May;32(5):496-503. [Content Brief]

Complete Stock Solution Preparation Table

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg

DMSO / H₂O	1 mM	1.6399 mL	8.1993 mL	16.3986 mL	40.9964 mL
	5 mM	0.3280 mL	1.6399 mL	3.2797 mL	8.1993 mL
	10 mM	0.1640 mL	0.8199 mL	1.6399 mL	4.0996 mL
	15 mM	0.1093 mL	0.5466 mL	1.0932 mL	2.7331 mL
	20 mM	0.0820 mL	0.4100 mL	0.8199 mL	2.0498 mL
	25 mM	0.0656 mL	0.3280 mL	0.6559 mL	1.6399 mL
	30 mM	0.0547 mL	0.2733 mL	0.5466 mL	1.3665 mL
	40 mM	0.0410 mL	0.2050 mL	0.4100 mL	1.0249 mL
	50 mM	0.0328 mL	0.1640 mL	0.3280 mL	0.8199 mL
	60 mM	0.0273 mL	0.1367 mL	0.2733 mL	0.6833 mL
	80 mM	0.0205 mL	0.1025 mL	0.2050 mL	0.5125 mL
	100 mM	0.0164 mL	0.0820 mL	0.1640 mL	0.4100 mL

* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

Tiamulin fumarate Related Classifications

Help & FAQs

Do most proteins show cross-species activity?
Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

Tiamulin55297-96-6ThiamutilinBacterialAntibioticInhibitorinhibitorinhibit

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