2. Anti-infection
  3. Bacterial Antibiotic
  4. Tiamulin

Tiamulin  (Synonyms: Thiamutilin)

Cat. No.: HY-B2060 Purity: 98.07%
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Tiamulin (Thiamutilin) is a diterpenic antibiotic that is widely used in pigs and poultry for the control of infectious diseases. Tiamulin is effectively used in the study of airsacculitis, which is primarily caused by Mycoplasma spp.

For research use only. We do not sell to patients.

Tiamulin Chemical Structure

Tiamulin Chemical Structure

CAS No. : 55297-95-5

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250 mg USD 66 In-stock
1 g USD 132 In-stock
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Based on 1 publication(s) in Google Scholar

Other Forms of Tiamulin:

Tiamulin Related Antibodies

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  • Biological Activity

  • Purity & Documentation

  • References

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Description

Tiamulin (Thiamutilin) is a diterpenic antibiotic that is widely used in pigs and poultry for the control of infectious diseases. Tiamulin is effectively used in the study of airsacculitis, which is primarily caused by Mycoplasma spp[1].

In Vitro

Tiamulin is a semisynthetic derivative of the diterpene antibiotic Pleuromutilin and is effectively used in the study of airsacculitis, which is primarily caused by Mycoplasma spp[1].
Tiamulin is highly active in vitro against Mycoplasma strains (M. gallisepticum, M. synoviae, M. meleagridis, and M. iowae), Spirochaetes (Brachyspira hyodysenteriae, Brachyspira innocens, B. pilosicoli, B. intermedia), gram-positive bacteria (staphylococci, streptococci, Clostridia, Arcanobacterium spp), but less active against gram-negative bacteria (Pasteurella, Klebsiella, Haemophilus, Fusobacterium, Campylobacter, Bacteroides spp.)[1].
Tiamulin binds with the rRNA in the peptidyl transferase slot on the ribosome, in which it prevents the correct positioning of the CCA ends of tRNA for peptide transferase and subsequent protein production[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Tiamulin Related Antibodies
In Vivo

Tiamulin is highly effective in the treatment of avian intestinal spirochaetosis in breeder and layer hens at 25 mg/kg of BW per day over 5 d in artificial infection studies with B. pilosicoli and B. intermedia, respectively[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
Molecular Weight

493.74

Formula

C28H47NO4S
CAS No.
Appearance

Oil

Color

Light yellow to brown

SMILES

O=C(O[C@H]1[C@@]([C@H](C)CC2)(C)[C@@](C(CC3)=O)([H])[C@]32[C@@H](C)[C@H](O)[C@](C)(C=C)C1)CSCCN(CC)CC
Shipping

Room temperature in continental US; may vary elsewhere.
Storage
Pure form -20°C 3 years
4°C 2 years
In solvent -80°C 6 months
-20°C 1 month
Solvent & Solubility
In Vitro: 

DMSO : 100 mg/mL (202.54 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Preparing
Stock Solutions
Concentration Solvent Mass 1 mg 5 mg 10 mg
1 mM 2.0254 mL 10.1268 mL 20.2536 mL
5 mM 0.4051 mL 2.0254 mL 4.0507 mL
View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

  • Molarity Calculator

  • Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Mass
=
Concentration
×
Volume
×
Molecular Weight *

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start)

C1

×
Volume (start)

V1

=
Concentration (final)

C2

×
Volume (final)

V2

In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

  • Protocol 1

    Add each solvent one by one:  10% DMSO    40% PEG300    5% Tween-80    45% Saline

    Solubility: ≥ 5 mg/mL (10.13 mM); Clear solution

    This protocol yields a clear solution of ≥ 5 mg/mL (saturation unknown).

    Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (50.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.

    Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
  • Protocol 2

    Add each solvent one by one:  10% DMSO    90% Corn Oil

    Solubility: ≥ 5 mg/mL (10.13 mM); Clear solution

    This protocol yields a clear solution of ≥ 5 mg/mL (saturation unknown). If the continuous dosing period exceeds half a month, please choose this protocol carefully.

    Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (50.0 mg/mL) to 900 μL Corn oil, and mix evenly.

In Vivo Dissolution Calculator
Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

g

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Please enter your animal formula composition:
%
DMSO +
% 
+
%
Tween-80 +
%
Saline
Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).
Calculation results:
Working solution concentration: mg/mL
Method for preparing stock solution: mg drug dissolved in μL  DMSO (Stock solution concentration: mg/mL).
The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only. If necessary, please contact MedChemExpress (MCE).
Method for preparing in vivo working solution for animal experiments: Take μL DMSO stock solution, add μL . μL , mix evenly, next add μL Tween 80, mix evenly, then add μL Saline.
 If the continuous dosing period exceeds half a month, please choose this protocol carefully.
Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.
Purity & Documentation
References

Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
DMSO 1 mM 2.0254 mL 10.1268 mL 20.2536 mL 50.6339 mL
5 mM 0.4051 mL 2.0254 mL 4.0507 mL 10.1268 mL
10 mM 0.2025 mL 1.0127 mL 2.0254 mL 5.0634 mL
15 mM 0.1350 mL 0.6751 mL 1.3502 mL 3.3756 mL
20 mM 0.1013 mL 0.5063 mL 1.0127 mL 2.5317 mL
25 mM 0.0810 mL 0.4051 mL 0.8101 mL 2.0254 mL
30 mM 0.0675 mL 0.3376 mL 0.6751 mL 1.6878 mL
40 mM 0.0506 mL 0.2532 mL 0.5063 mL 1.2658 mL
50 mM 0.0405 mL 0.2025 mL 0.4051 mL 1.0127 mL
60 mM 0.0338 mL 0.1688 mL 0.3376 mL 0.8439 mL
80 mM 0.0253 mL 0.1266 mL 0.2532 mL 0.6329 mL
100 mM 0.0203 mL 0.1013 mL 0.2025 mL 0.5063 mL
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Tiamulin Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

Tiamulin55297-95-5ThiamutilinBacterialAntibioticveterinaryswinedysenteryenzooticpneumoniaInhibitorinhibitorinhibit

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