Tirapazamine (Synonyms: SR259075; SR4233; Win59075; SML 0552; SR 259075; Tirazone)

Name: Tirapazamine
Brand: MedChemExpress
SKU: HY-13767
Rating: 5.0 (10 reviews)

Cat. No.: HY-13767 Purity: 99.73%: FAQs
Data Sheet SDS COA Handling Instructions

Molarity Calculator

Tirapazamine (SR259075) is an anticancer agent that shows selective cytotoxicity for hypoxic cells in solid tumors, thereby inducing single-and double-strand breaks in DNA, base damage, and cell death. Tirapazamine is an anticancer and bioreductive agent.Tirapazamine (SR259075) can enhance the cytotoxic effects of ionizing radiation in hypoxic cells.

For research use only. We do not sell to patients.

Tirapazamine Chemical Structure

CAS No. : 27314-97-2

Size	Price	Stock	Quantity

Free Sample (0.1 - 0.5 mg)		Apply Now
Solid + Solvent (Highly Recommended)
10 mM * 1 mL in DMSO ready for reconstitution	USD 87	In-stock	Estimated Time of Arrival: December 31
Solution
10 mM * 1 mL in DMSO	USD 87	In-stock	Estimated Time of Arrival: December 31
Solid
50 mg	USD 79	In-stock	Estimated Time of Arrival: December 31
100 mg	USD 145	In-stock	Estimated Time of Arrival: December 31
200 mg	USD 264	In-stock	Estimated Time of Arrival: December 31
500 mg		Get quote
1 g		Get quote

or Bulk Inquiry

* Please select Quantity before adding items.

This product is a controlled substance and not for sale in your territory.

Customer Review

Based on 10 publication(s) in Google Scholar

10 Publications Citing Use of MCE Tirapazamine

Featured Recommendations

•Related Screening Libraries:

•Related Small Molecules:

•Related Proteins:

Biological Activity
Purity & Documentation
References
Customer Review

Description

Cellular Effect

Cell Line	Type	Value	Description	References
A549	IC₅₀	> 50 μM Compound: TPZ	Antiproliferative activity against human A549 cells assessed as inhibition of cell proliferation under normoxia conditions after 72 hrs by SRB assay Antiproliferative activity against human A549 cells assessed as inhibition of cell proliferation under normoxia conditions after 72 hrs by SRB assay	[PMID: 25468044]
A549	IC₅₀	1.93 μM Compound: TPZ	Cytotoxicity against human A549 cells for 72 hrs under hypoxic condition by MTT assay Cytotoxicity against human A549 cells for 72 hrs under hypoxic condition by MTT assay	[PMID: 21281992]
A549	GI₅₀	6.8 μM Compound: Tirapazamine	Cytotoxicity against human A549 cells incubated for 4 hrs in anoxic condition followed by oxic exposure for 48 hrs by sulforhodamine B assay Cytotoxicity against human A549 cells incubated for 4 hrs in anoxic condition followed by oxic exposure for 48 hrs by sulforhodamine B assay	[PMID: 32196334]
A549	IC₅₀	6.9 μM Compound: TPZ	Antiproliferative activity against human A549 cells assessed as inhibition of cell proliferation under hypoxia conditions after 72 hrs by SRB assay Antiproliferative activity against human A549 cells assessed as inhibition of cell proliferation under hypoxia conditions after 72 hrs by SRB assay	[PMID: 25468044]
A549	GI₅₀	63 μM Compound: Tirapazamine	Cytotoxicity against human A549 cells incubated for 52 hrs in oxic condition by sulforhodamine B assay Cytotoxicity against human A549 cells incubated for 52 hrs in oxic condition by sulforhodamine B assay	[PMID: 32196334]
A549	IC₅₀	7.43 μM Compound: TPZ	Cytotoxicity against human A549 cells for 72 hrs under normoxic condition by MTT assay Cytotoxicity against human A549 cells for 72 hrs under normoxic condition by MTT assay	[PMID: 21281992]
COLO 205	IC₅₀	47.7 μM Compound: Tirapazamine	Cytotoxicity against human COLO205 cells measured after 48 hrs under hypoxic condition by CCK8 assay Cytotoxicity against human COLO205 cells measured after 48 hrs under hypoxic condition by CCK8 assay	[PMID: 27140429]
H9c2	EC₅₀	> 100 μM Compound: 10; TPZ	Cytotoxicity against rat H9c2 cells assessed as reduction in cell viability incubated for 24 hrs under 19% O2 condition by Hoechst 33342 staining based microscopic analysis Cytotoxicity against rat H9c2 cells assessed as reduction in cell viability incubated for 24 hrs under 19% O2 condition by Hoechst 33342 staining based microscopic analysis	[PMID: 34124675]
HCT-116	IC₅₀	11.9 μM Compound: Tirapazamine	Cytotoxicity against human HCT116 cells measured after 48 hrs under hypoxic condition by CCK8 assay Cytotoxicity against human HCT116 cells measured after 48 hrs under hypoxic condition by CCK8 assay	[PMID: 27140429]
HCT-116	IC₅₀	72.7 μM Compound: Tirapazamine	Cytotoxicity against human HCT116 cells measured after 18 hrs under hypoxic condition by CCK8 assay Cytotoxicity against human HCT116 cells measured after 18 hrs under hypoxic condition by CCK8 assay	[PMID: 27140429]
HepG2	IC₅₀	19.1 μM Compound: TPZ	Cytotoxicity against human HepG2 cells after 48 hrs by MTT assay Cytotoxicity against human HepG2 cells after 48 hrs by MTT assay	[PMID: 16777409]
HepG2	IC₅₀	56.6 μg/mL Compound: TPZ	Growth inhibition of human HepG2 cells after 48 hrs by SRB assay Growth inhibition of human HepG2 cells after 48 hrs by SRB assay	[PMID: 20236735]
HL-60	IC₅₀	16.1 μM Compound: TPZ	Cytotoxicity against human HL60 cells in normoxia after 48 hrs Cytotoxicity against human HL60 cells in normoxia after 48 hrs	[PMID: 16777409]
HL-60	IC₅₀	2.31 μM Compound: TPZ	Ratio of cytotoxicity against human HL60 cells in normoxia to hypoxia after 48 hrs Ratio of cytotoxicity against human HL60 cells in normoxia to hypoxia after 48 hrs	[PMID: 16777409]
HL-60	IC₅₀	7 μM Compound: TPZ	Cytotoxicity against human HL60 cells after 48 hrs by MTT assay Cytotoxicity against human HL60 cells after 48 hrs by MTT assay	[PMID: 16777409]
HL-60	IC₅₀	7 μM Compound: TPZ	Cytotoxicity against human HL60 cells in hypoxia after 48 hrs Cytotoxicity against human HL60 cells in hypoxia after 48 hrs	[PMID: 16777409]
HT-29	IC₅₀	> 50 μM Compound: TPZ	Antiproliferative activity against human HT-29 cells assessed as inhibition of cell proliferation under normoxia conditions after 72 hrs by SRB assay Antiproliferative activity against human HT-29 cells assessed as inhibition of cell proliferation under normoxia conditions after 72 hrs by SRB assay	[PMID: 25468044]
HT-29	IC₅₀	> 50 μM Compound: TPZ	Cytotoxicity against human HT-29 cells after 72 hrs under normoxia conditions by SRB assay Cytotoxicity against human HT-29 cells after 72 hrs under normoxia conditions by SRB assay	[PMID: 25462282]
HT-29	IC₅₀	387 μM Compound: TPZ	Antiproliferative activity against human HT-29 cells assessed as inhibition of cell growth under normoxia condition by SRB assay Antiproliferative activity against human HT-29 cells assessed as inhibition of cell growth under normoxia condition by SRB assay	[PMID: 28851503]
HT-29	IC₅₀	5.1 μM Compound: 1, TPZ	Cytotoxicity against human HT29 cells under hypoxic conditions after 4 hrs Cytotoxicity against human HT29 cells under hypoxic conditions after 4 hrs	[PMID: 18001018]
HT-29	IC₅₀	5.1 μM Compound: 1, TPZ	Cytotoxicity against human HT-29 cells after 4 hrs under strict hypoxic condition Cytotoxicity against human HT-29 cells after 4 hrs under strict hypoxic condition	[PMID: 18847185]
HT-29	IC₅₀	6.2 μM Compound: TPZ	Antiproliferative activity against human HT-29 cells assessed as inhibition of cell growth under hypoxia condition by SRB assay Antiproliferative activity against human HT-29 cells assessed as inhibition of cell growth under hypoxia condition by SRB assay	[PMID: 28851503]
HT-29	IC₅₀	9.45 μM Compound: TPZ	Antiproliferative activity against human HT-29 cells assessed as inhibition of cell proliferation under hypoxia conditions after 72 hrs by SRB assay Antiproliferative activity against human HT-29 cells assessed as inhibition of cell proliferation under hypoxia conditions after 72 hrs by SRB assay	[PMID: 25468044]
HT-29	IC₅₀	9.45 μM Compound: TPZ	Cytotoxicity against human HT-29 cells after 72 hrs under hypoxia conditions by SRB assay Cytotoxicity against human HT-29 cells after 72 hrs under hypoxia conditions by SRB assay	[PMID: 25462282]
K562	IC₅₀	1.81 μM Compound: TPZ	Cytotoxicity against human K562 cells for 72 hrs under hypoxic condition by MTT assay Cytotoxicity against human K562 cells for 72 hrs under hypoxic condition by MTT assay	[PMID: 21281992]
K562	IC₅₀	19.41 μM Compound: TPZ	Cytotoxicity against human K562 cells for 72 hrs under normoxic condition by MTT assay Cytotoxicity against human K562 cells for 72 hrs under normoxic condition by MTT assay	[PMID: 21281992]
K562	IC₅₀	5.2 μM Compound: TPZ	Cytotoxicity against human K562 cells after 48 hrs by MTT assay Cytotoxicity against human K562 cells after 48 hrs by MTT assay	[PMID: 16777409]
KB	IC₅₀	18.71 μM Compound: TPZ	Cytotoxicity against human KB cells for 72 hrs under hypoxic condition by MTT assay Cytotoxicity against human KB cells for 72 hrs under hypoxic condition by MTT assay	[PMID: 21281992]
KB	IC₅₀	6.29 μM Compound: TPZ	Cytotoxicity against human KB cells for 72 hrs under normoxic condition by MTT assay Cytotoxicity against human KB cells for 72 hrs under normoxic condition by MTT assay	[PMID: 21281992]
MDA-MB-468	IC₅₀	1.304 μM Compound: Tirapazamine	Cytotoxicity against human MDA-MB-468 cells incubated for 4 hrs under hypoxic condition followed by compound washout and measured after 5 days by SRB assay Cytotoxicity against human MDA-MB-468 cells incubated for 4 hrs under hypoxic condition followed by compound washout and measured after 5 days by SRB assay	[PMID: 30885680]
MDA-MB-468	IC₅₀	105.2 μM Compound: Tirapazamine	Cytotoxicity against human MDA-MB-468 cells incubated for 4 hrs under aerobic condition followed by compound washout and measured after 5 days by SRB assay Cytotoxicity against human MDA-MB-468 cells incubated for 4 hrs under aerobic condition followed by compound washout and measured after 5 days by SRB assay	[PMID: 30885680]
MDCK	IC₅₀	< 50 μM Compound: Tirapazamine	Cytotoxicity against carbonic anhydrase 9 knockdown MDCK cells assessed as reduction in cell viability after 2 hrs under hypoxic condition by Alamar blue assay Cytotoxicity against carbonic anhydrase 9 knockdown MDCK cells assessed as reduction in cell viability after 2 hrs under hypoxic condition by Alamar blue assay	[PMID: 27823879]
MDCK	IC₅₀	> 300 μM Compound: Tirapazamine	Cytotoxicity against carbonic anhydrase 9 knockdown MDCK cells assessed as reduction in cell viability preincubated for 2 hrs followed by 72 hrs incubation under normoxic condition by Alamar blue assay Cytotoxicity against carbonic anhydrase 9 knockdown MDCK cells assessed as reduction in cell viability preincubated for 2 hrs followed by 72 hrs incubation under normoxic condition by Alamar blue assay	[PMID: 27823879]
MDCK	IC₅₀	> 300 μM Compound: Tirapazamine	Cytotoxicity against MDCK cells expressing carbonic anhydrase 9 assessed as reduction in cell viability preincubated for 2 hrs followed by 72 hrs incubation under normoxic condition by Alamar blue assay Cytotoxicity against MDCK cells expressing carbonic anhydrase 9 assessed as reduction in cell viability preincubated for 2 hrs followed by 72 hrs incubation under normoxic condition by Alamar blue assay	[PMID: 27823879]
MDCK	IC₅₀	95 μM Compound: Tirapazamine	Cytotoxicity against MDCK cells expressing carbonic anhydrase 9 assessed as reduction in cell viability after 2 hrs under hypoxic condition by Alamar blue assay Cytotoxicity against MDCK cells expressing carbonic anhydrase 9 assessed as reduction in cell viability after 2 hrs under hypoxic condition by Alamar blue assay	[PMID: 27823879]
MOLM-13	EC₅₀	22 μM Compound: 10; TPZ	Cytotoxicity against human MOLM13 cells assessed as reduction in cell viability incubated for 24 hrs under 2% O2 condition by Hoechst 33342 staining based microscopic analysis Cytotoxicity against human MOLM13 cells assessed as reduction in cell viability incubated for 24 hrs under 2% O2 condition by Hoechst 33342 staining based microscopic analysis	[PMID: 34124675]
MOLM-13	EC₅₀	22 μM Compound: 4; TPZ	Antiproliferative activity against human MOLM13 cells after 24 hrs under 2% O2 condition by Hoechst 33342 staining-based UV-microscopic analysis Antiproliferative activity against human MOLM13 cells after 24 hrs under 2% O2 condition by Hoechst 33342 staining-based UV-microscopic analysis	[PMID: 28284865]
MOLM-13	EC₅₀	95 μM Compound: 10; TPZ	Cytotoxicity against human MOLM13 cells assessed as reduction in cell viability incubated for 24 hrs under 19% O2 condition by Hoechst 33342 staining based microscopic analysis Cytotoxicity against human MOLM13 cells assessed as reduction in cell viability incubated for 24 hrs under 19% O2 condition by Hoechst 33342 staining based microscopic analysis	[PMID: 34124675]
MOLM-13	EC₅₀	95 μM Compound: 4; TPZ	Antiproliferative activity against human MOLM13 cells after 24 hrs under 19% O2 condition by Hoechst 33342 staining-based UV-microscopic analysis Antiproliferative activity against human MOLM13 cells after 24 hrs under 19% O2 condition by Hoechst 33342 staining-based UV-microscopic analysis	[PMID: 28284865]
MOLT-4	IC₅₀	15.8 μM Compound: TPZ	Cytotoxicity against human Molt4 cells in normoxia after 48 hrs Cytotoxicity against human Molt4 cells in normoxia after 48 hrs	[PMID: 16777409]
MOLT-4	IC₅₀	3.43 μM Compound: TPZ	Ratio of cytotoxicity against human Molt4 cells in normoxia to hypoxia after 48 hrs Ratio of cytotoxicity against human Molt4 cells in normoxia to hypoxia after 48 hrs	[PMID: 16777409]
MOLT-4	IC₅₀	4.6 μM Compound: TPZ	Cytotoxicity against human Molt4 cells after 48 hrs by MTT assay Cytotoxicity against human Molt4 cells after 48 hrs by MTT assay	[PMID: 16777409]
MOLT-4	IC₅₀	4.6 μM Compound: TPZ	Cytotoxicity against human Molt4 cells in hypoxia after 48 hrs Cytotoxicity against human Molt4 cells in hypoxia after 48 hrs	[PMID: 16777409]
NCI-H460	IC₅₀	> 100 μM Compound: tirapazamine	Cytotoxicity against human H460 cells under normoxic condition after 2 hrs by Alamar blue staining assay Cytotoxicity against human H460 cells under normoxic condition after 2 hrs by Alamar blue staining assay	[PMID: 18257544]
NCI-H460	IC₅₀	11 μM Compound: tirapazamine	Cytotoxicity against human H460 cells under hypoxic condition after 2 hrs by Alamar blue staining assay Cytotoxicity against human H460 cells under hypoxic condition after 2 hrs by Alamar blue staining assay	[PMID: 18257544]
NRK	EC₅₀	> 100 μM Compound: 10; TPZ	Cytotoxicity against rat NRK cells assessed as reduction in cell viability incubated for 24 hrs under 19% O2 condition by Hoechst 33342 staining based microscopic analysis Cytotoxicity against rat NRK cells assessed as reduction in cell viability incubated for 24 hrs under 19% O2 condition by Hoechst 33342 staining based microscopic analysis	[PMID: 34124675]
NRK	EC₅₀	35 μM Compound: 10; TPZ	Cytotoxicity against rat NRK cells assessed as reduction in cell viability incubated for 24 hrs under 2% O2 condition by Hoechst 33342 staining based microscopic analysis Cytotoxicity against rat NRK cells assessed as reduction in cell viability incubated for 24 hrs under 2% O2 condition by Hoechst 33342 staining based microscopic analysis	[PMID: 34124675]
PC-3	IC₅₀	1.17 μM Compound: TPZ	Cytotoxicity against human PC3 cells for 72 hrs under hypoxic condition by MTT assay Cytotoxicity against human PC3 cells for 72 hrs under hypoxic condition by MTT assay	[PMID: 21281992]
PC-3	IC₅₀	20.97 μM Compound: TPZ	Cytotoxicity against human PC3 cells for 72 hrs under normoxic condition by MTT assay Cytotoxicity against human PC3 cells for 72 hrs under normoxic condition by MTT assay	[PMID: 21281992]
PC-3	IC₅₀	22.3 μM Compound: TPZ	Cytotoxicity against human PC3 cells after 48 hrs by MTT assay Cytotoxicity against human PC3 cells after 48 hrs by MTT assay	[PMID: 16777409]
SiHa	IC₅₀	2.5 μM Compound: 1, TPZ	Cytotoxicity against human SiHa cells under hypoxic conditions after 4 hrs Cytotoxicity against human SiHa cells under hypoxic conditions after 4 hrs	[PMID: 18001018]
SiHa	IC₅₀	2.5 μM Compound: 1, TPZ	Cytotoxicity against human SiHa cells after 4 hrs under strict hypoxic condition Cytotoxicity against human SiHa cells after 4 hrs under strict hypoxic condition	[PMID: 18847185]
SMMC-7721	IC₅₀	32.79 μM Compound: TPZ	Cytotoxicity against human SMMC7721 cells for 72 hrs under normoxic condition by MTT assay Cytotoxicity against human SMMC7721 cells for 72 hrs under normoxic condition by MTT assay	[PMID: 21281992]
SMMC-7721	IC₅₀	4.75 μM Compound: TPZ	Cytotoxicity against human SMMC7721 cells for 72 hrs under hypoxic condition by MTT assay Cytotoxicity against human SMMC7721 cells for 72 hrs under hypoxic condition by MTT assay	[PMID: 21281992]
SW-620	IC₅₀	1.2 μM Compound: Tirapazamine	Cytotoxicity against human SW620 cells incubated for 4 hrs under hypoxic condition followed by compound washout and measured after 5 days by SRB assay Cytotoxicity against human SW620 cells incubated for 4 hrs under hypoxic condition followed by compound washout and measured after 5 days by SRB assay	[PMID: 30885680]
SW-620	IC₅₀	91.03 μM Compound: Tirapazamine	Cytotoxicity against human SW620 cells incubated for 4 hrs under aerobic condition followed by compound washout and measured after 5 days by SRB assay Cytotoxicity against human SW620 cells incubated for 4 hrs under aerobic condition followed by compound washout and measured after 5 days by SRB assay	[PMID: 30885680]
U-251	IC₅₀	71.2 μg/mL Compound: TPZ	Growth inhibition of human U251 cells after 48 hrs by SRB assay Growth inhibition of human U251 cells after 48 hrs by SRB assay	[PMID: 20236735]
Vero	CC₅₀	8 μg/mL Compound: TPZ	Cytotoxicity against african green monkey Vero cells after 72 hrs by CellTiterGlo assay Cytotoxicity against african green monkey Vero cells after 72 hrs by CellTiterGlo assay	[PMID: 22691154]

In Vitro

Tirapazamine (SR259075) is the optimal drug for combination therapy using Pba^[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Cell Viability Assay^[1]

Cell Line:	SCC7 cells
Concentration:	1mg
Incubation Time:	24 h
Result:	Showed synergism with Pba at ED50, ED90 and ED95.

In Vivo

Tirapazamine (SR259075) (1mg; intravenously injected; twice at a 24-h interval) shows a synergetic effect to kill tumor cells because TPZ was activated under the hypoxic conditions that originated from the PDT with Pba and laser irradiation^[1].
.

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model:	C3H/HeN mice^[1].
Dosage:	1mg
Administration:	Tirapazamine (1mg; intravenously injected; twice at a 24-h interval)
Result:	Suppressed the tumors of mice by using laser irradiation.

Clinical Trial

Molecular Weight

178.15

Formula

C₇H₆N₄O₂

CAS No.

27314-97-2

Appearance

Solid

Color

Orange to red

SMILES

NC1=[N+]([O-])C2=CC=CC=C2[N+]([O-])=N1

Shipping

Room temperature in continental US; may vary elsewhere.

Storage

Powder	-20°C	3 years
	4°C	2 years
In solvent	-80°C	6 months
	-20°C	1 month

Solvent & Solubility

In Vitro:

DMSO : 62.5 mg/mL (350.83 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Preparing
Stock Solutions

Concentration Solvent Mass	1 mg	5 mg	10 mg

1 mM	5.6132 mL	28.0662 mL	56.1325 mL
5 mM	1.1226 mL	5.6132 mL	11.2265 mL
10 mM	0.5613 mL	2.8066 mL	5.6132 mL

View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Molarity Calculator
Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

This equation is commonly abbreviated as: C₁V₁ = C₂V₂

Concentration _(start)

Volume _(start)

Concentration _(final)

Volume _(final)

In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

Protocol 1

Add each solvent one by one: 10% DMSO 90% Corn Oil
Solubility: 2.5 mg/mL (14.03 mM); Suspended solution; Need ultrasonic

This protocol yields a suspended solution of 2.5 mg/mL. Suspended solution can be used for oral and intraperitoneal injection.
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL Corn oil, and mix evenly.
Protocol 2

Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline
Solubility: ≥ 2.08 mg/mL (11.68 mM); Clear solution

This protocol yields a clear solution of ≥ 2.08 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (20.8 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.
Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
Protocol 3

Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in Saline)
Solubility: ≥ 2.08 mg/mL (11.68 mM); Clear solution

This protocol yields a clear solution of ≥ 2.08 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (20.8 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.
Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.

For the following dissolution methods, please prepare the working solution directly. It is recommended to prepare fresh solutions and use them promptly within a short period of time.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

Protocol 1

Add each solvent one by one: 50% PEG300 50% Saline
Solubility: 10 mg/mL (56.13 mM); Suspended solution; Need ultrasonic

In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Please enter your animal formula composition:

DMSO +

Tween-80 +

Saline

Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.

The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).

Calculation results:

Working solution concentration: mg/mL

Method for preparing stock solution: mg drug dissolved in μL DMSO (Stock solution concentration: mg/mL).

The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only. If necessary, please contact MedChemExpress (MCE).

Method for preparing in vivo working solution for animal experiments: Take μL DMSO stock solution, add μL . μL , mix evenly, next add μL Tween 80, mix evenly, then add μL Saline.

If the continuous dosing period exceeds half a month, please choose this protocol carefully.

Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.

Purity & Documentation

Purity: 99.73%

Select Batch:

Data Sheet (278 KB) SDS (393 KB)

COA (250 KB) LCMS (111 KB)

Handling Instructions (2659 KB)

References

[1]. Cai TY, et al. Tirapazamine sensitizes hepatocellular carcinoma cells to topoisomerase I inhibitors via cooperative modulation of hypoxia-inducible factor-1α. Mol Cancer Ther. 2014 Mar;13(3):630-42. [Content Brief]

[2]. Sliwinska J, et al. Tirapazamine-doxorubicin interaction referring to heart oxidative stress and Ca2? balance protein levels. Oxid Med Cell Longev. 2012;2012:890826. [Content Brief]

[3]. Lee, Donghyun, et al. Optimized Combination of Photodynamic Therapy and Chemotherapy Using Gelatin Nanoparticles Containing Tirapazamine and Pheophorbide a. ACS applied materials & interfaces vol. 13,9 (2021): 10812-10821. [Content Brief]

[4]. Romero, José, et al. Electronic structure and reactivity of tirapazamine as a radiosensitizer. Journal of molecular modeling vol. 27,6 177. 22 May. 2021. [Content Brief]

Complete Stock Solution Preparation Table

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg

DMSO	1 mM	5.6132 mL	28.0662 mL	56.1325 mL	140.3312 mL
	5 mM	1.1226 mL	5.6132 mL	11.2265 mL	28.0662 mL
	10 mM	0.5613 mL	2.8066 mL	5.6132 mL	14.0331 mL
	15 mM	0.3742 mL	1.8711 mL	3.7422 mL	9.3554 mL
	20 mM	0.2807 mL	1.4033 mL	2.8066 mL	7.0166 mL
	25 mM	0.2245 mL	1.1226 mL	2.2453 mL	5.6132 mL
	30 mM	0.1871 mL	0.9355 mL	1.8711 mL	4.6777 mL
	40 mM	0.1403 mL	0.7017 mL	1.4033 mL	3.5083 mL
	50 mM	0.1123 mL	0.5613 mL	1.1226 mL	2.8066 mL
	60 mM	0.0936 mL	0.4678 mL	0.9355 mL	2.3389 mL
	80 mM	0.0702 mL	0.3508 mL	0.7017 mL	1.7541 mL
	100 mM	0.0561 mL	0.2807 mL	0.5613 mL	1.4033 mL

Tirapazamine Related Classifications

Help & FAQs

Do most proteins show cross-species activity?
Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Please select a local distributor ナミキ商事株式会社コスモ・バイオ株式会社
Product Name			Salutation
Applicant Name *			Email Address *
Phone Number *			Organization Name *
Department *			Requested quantity *
Country or Region *
Remarks

Product Name			Lot #
Applicant Name *			Email Address *
Phone Number			Department *
Organization Name *			Country or Region *
Remarks

Tirapazamine (Synonyms: SR259075; SR4233; Win59075; SML 0552; SR 259075; Tirazone)

Customer Review

10 Publications Citing Use of MCE Tirapazamine

Featured Recommendations

•Related Screening Libraries:

•Related Small Molecules:

•Related Proteins:

Biological Activity

Purity & Documentation

References

Customer Review

Molarity Calculator

Dilution Calculator

Complete Stock Solution Preparation Table

Tirapazamine Related Classifications

Keywords:

Your Recently Viewed Products:

Name
Email *

	Sorry, but the email address you supplied was invalid.

Tirapazamine (Synonyms: SR259075; SR4233; Win59075; SML 0552; SR 259075; Tirazone)

Customer Review

Tirapazamine Related Antibodies

10 Publications Citing Use of MCE Tirapazamine

Featured Recommendations

•Related Screening Libraries:

•Related Small Molecules:

•Related Proteins:

Biological Activity

Purity & Documentation

References

Customer Review

Tirapazamine Related Antibodies

Molarity Calculator

Dilution Calculator

Complete Stock Solution Preparation Table

Tirapazamine Related Classifications

Keywords:

Your Recently Viewed Products:

Customer Review

Request for HNMR Report

SAFETY DATA SHEET (SDS)

Request for HNMR Report