2. Metabolic Enzyme/Protease
  3. Phosphodiesterase (PDE)
  4. (R)-(-)-Rolipram

(R)-(-)-Rolipram  (Synonyms: (R)-Rolipram; (-)-Rolipram)

Cat. No.: HY-16900A Purity: 99.89%
COA Handling Instructions

Molarity Calculator

(R)-(-)-Rolipram is the R-enantiomer of Rolipram. Rolipram is a selective inhibitor of phosphodiesterases PDE4 with IC50 of 3 nM, 130 nM and 240 nM for PDE4A, PDE4B, and PDE4D, respectively.

For research use only. We do not sell to patients.

(R)-(-)-Rolipram Chemical Structure

(R)-(-)-Rolipram Chemical Structure

CAS No. : 85416-75-7

Size Price Stock Quantity
Solid + Solvent (Highly Recommended)
10 mM * 1 mL in DMSO
ready for reconstitution
 USD 133 In-stock
Solution
10 mM * 1 mL in DMSO USD 133 In-stock
Solid
1 mg USD 31 In-stock
5 mg USD 78 In-stock
10 mg USD 121 In-stock
50 mg USD 429 In-stock
100 mg   Get quote  
200 mg   Get quote  

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This product is a controlled substance and not for sale in your territory.

Customer Review

Based on 3 publication(s) in Google Scholar

Other Forms of (R)-(-)-Rolipram:

(R)-(-)-Rolipram Related Antibodies

Top Publications Citing Use of Products

2 Publications Citing Use of MCE (R)-(-)-Rolipram

WB

    (R)-(-)-Rolipram purchased from MedChemExpress. Usage Cited in: Immunity. 2016 Oct 18;45(4):944.  [Abstract]

    LPS-primed BMDMs are treated with different doses of (R)-(-)-Rolipram, and then stimulated with Nigericin for 45 min. Supernatants (SN) and cell extracts (Lysate) are analyzed by immunoblotting. The phosphodiesterase inhibitors isobutylmethylxanthine (IBMX) and (R)-(-)-rolipram suppresses caspase-1 cleavage and IL-1β secretion in a dose-dependent manner.

    View All Phosphodiesterase (PDE) Isoform Specific Products:

    View All Isoforms
    PDE1 PDE2 PDE3 PDE4 PDE5 PDE6 PDE7 PDE9 PDE10 PDE11 PDE12 PDE8 Autotaxin

    • Biological Activity

    • Purity & Documentation

    • References

    • Customer Review

    Description

    (R)-(-)-Rolipram is the R-enantiomer of Rolipram. Rolipram is a selective inhibitor of phosphodiesterases PDE4 with IC50 of 3 nM, 130 nM and 240 nM for PDE4A, PDE4B, and PDE4D, respectively.

    In Vitro

    The increase of cAMP synthesis with the adenylate cyclase activator forskolin or the decrease of cAMP hydrolysis by the phosphodiesterase inhibitors Isobutylmethylxanthine (IBMX) and (R)-(-)-Rolipram (MedChem Express) suppresses caspase-1 cleavage and IL-1β secretion in a dose-dependent manner[1].

    MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

    (R)-(-)-Rolipram Related Antibodies
    Molecular Weight

    275.34

    Formula

    C16H21NO3
    CAS No.
    Appearance

    Solid

    Color

    Off-white to light yellow

    SMILES

    O=C1NC[C@@H](C2=CC=C(OC)C(OC3CCCC3)=C2)C1
    Shipping

    Room temperature in continental US; may vary elsewhere.
    Storage
    Powder -20°C 3 years
    4°C 2 years
    In solvent -80°C 2 years
    -20°C 1 year
    Solvent & Solubility
    In Vitro: 

    DMSO : 100 mg/mL (363.19 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

    Preparing
    Stock Solutions
    Concentration Solvent Mass 1 mg 5 mg 10 mg
    1 mM 3.6319 mL 18.1594 mL 36.3187 mL
    5 mM 0.7264 mL 3.6319 mL 7.2637 mL
    View the Complete Stock Solution Preparation Table

    * Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
    Storage method and period of stock solution: -80°C, 2 years; -20°C, 1 year. When stored at -80°C, please use it within 2 years. When stored at -20°C, please use it within 1 year.

    • Molarity Calculator

    • Dilution Calculator

    Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

    Mass
    =
    Concentration
    ×
    Volume
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    Molecular Weight *

    Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

    This equation is commonly abbreviated as: C1V1 = C2V2

    Concentration (start)

    C1

    ×
    Volume (start)

    V1

    =
    Concentration (final)

    C2

    ×
    Volume (final)

    V2

    In Vivo:

    Select the appropriate dissolution method based on your experimental animal and administration route.

    For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
    To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
    The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

    • Protocol 1

      Add each solvent one by one:  10% DMSO    40% PEG300    5% Tween-80    45% Saline

      Solubility: ≥ 2.5 mg/mL (9.08 mM); Clear solution

      This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).

      Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.

      Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
    • Protocol 2

      Add each solvent one by one:  10% DMSO    90% (20% SBE-β-CD in Saline)

      Solubility: ≥ 2.5 mg/mL (9.08 mM); Clear solution

      This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).

      Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.

      Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
    In Vivo Dissolution Calculator
    Please enter the basic information of animal experiments:

    Dosage

    mg/kg

    Animal weight
    (per animal)

    g

    Dosing volume
    (per animal)

    μL

    Number of animals

    Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
    Please enter your animal formula composition:
    %
    DMSO +
    % 
    +
    %
    Tween-80 +
    %
    Saline
    Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
    The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).
    Calculation results:
    Working solution concentration: mg/mL
    Method for preparing stock solution: mg drug dissolved in μL  DMSO (Stock solution concentration: mg/mL).
    The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only. If necessary, please contact MedChemExpress (MCE).
    Method for preparing in vivo working solution for animal experiments: Take μL DMSO stock solution, add μL . μL , mix evenly, next add μL Tween 80, mix evenly, then add μL Saline.
     If the continuous dosing period exceeds half a month, please choose this protocol carefully.
    Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.
    Purity & Documentation

    Purity: 99.89%

    COA (248 KB) HNMR (239 KB) LCMS (257 KB)

    Handling Instructions (2659 KB)
    References

    Complete Stock Solution Preparation Table

    * Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
    Storage method and period of stock solution: -80°C, 2 years; -20°C, 1 year. When stored at -80°C, please use it within 2 years. When stored at -20°C, please use it within 1 year.

    Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
    DMSO 1 mM 3.6319 mL 18.1594 mL 36.3187 mL 90.7968 mL
    5 mM 0.7264 mL 3.6319 mL 7.2637 mL 18.1594 mL
    10 mM 0.3632 mL 1.8159 mL 3.6319 mL 9.0797 mL
    15 mM 0.2421 mL 1.2106 mL 2.4212 mL 6.0531 mL
    20 mM 0.1816 mL 0.9080 mL 1.8159 mL 4.5398 mL
    25 mM 0.1453 mL 0.7264 mL 1.4527 mL 3.6319 mL
    30 mM 0.1211 mL 0.6053 mL 1.2106 mL 3.0266 mL
    40 mM 0.0908 mL 0.4540 mL 0.9080 mL 2.2699 mL
    50 mM 0.0726 mL 0.3632 mL 0.7264 mL 1.8159 mL
    60 mM 0.0605 mL 0.3027 mL 0.6053 mL 1.5133 mL
    80 mM 0.0454 mL 0.2270 mL 0.4540 mL 1.1350 mL
    100 mM 0.0363 mL 0.1816 mL 0.3632 mL 0.9080 mL
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    (R)-(-)-Rolipram Related Classifications

    Help & FAQs
    • Do most proteins show cross-species activity?

      Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

    Keywords:

    (R)-(-)-Rolipram85416-75-7(R)-Rolipram (-)-RolipramPhosphodiesterase (PDE)Inhibitorinhibitorinhibit

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