(R)-(-)-Rolipram (Synonyms: (R)-Rolipram; (-)-Rolipram)

Name: (R)-(-)-Rolipram
Brand: MedChemExpress
SKU: HY-16900A
Rating: 5.0 (3 reviews)

Cat. No.: HY-16900A Purity: 99.89%: FAQs
Data Sheet SDS COA Handling Instructions

Molarity Calculator

(R)-(-)-Rolipram is the R-enantiomer of Rolipram. Rolipram is a selective inhibitor of phosphodiesterases PDE4 with IC₅₀ of 3 nM, 130 nM and 240 nM for PDE4A, PDE4B, and PDE4D, respectively.

For research use only. We do not sell to patients.

(R)-(-)-Rolipram Chemical Structure

CAS No. : 85416-75-7

Size	Price	Stock	Quantity

Free Sample (0.1 - 0.5 mg)		Apply Now
Solid + Solvent (Highly Recommended)
10 mM * 1 mL in DMSO ready for reconstitution	USD 133	In-stock	Estimated Time of Arrival: December 31
Solution
10 mM * 1 mL in DMSO	USD 133	In-stock	Estimated Time of Arrival: December 31
Solid
5 mg	USD 78	In-stock	Estimated Time of Arrival: December 31
10 mg	USD 121	In-stock	Estimated Time of Arrival: December 31
50 mg	USD 429	In-stock	Estimated Time of Arrival: December 31
100 mg		Get quote
200 mg		Get quote

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Customer Review

Based on 3 publication(s) in Google Scholar

Other Forms of (R)-(-)-Rolipram:

Rolipram In-stock
(S)-(+)-Rolipram In-stock

2 Publications Citing Use of MCE (R)-(-)-Rolipram

: (R)-(-)-Rolipram purchased from MedChemExpress. Usage Cited in: Immunity. 2016 Oct 18;45(4):944. [Abstract]; LPS-primed BMDMs are treated with different doses of (R)-(-)-Rolipram, and then stimulated with Nigericin for 45 min. Supernatants (SN) and cell extracts (Lysate) are analyzed by immunoblotting. The phosphodiesterase inhibitors isobutylmethylxanthine (IBMX) and (R)-(-)-rolipram suppresses caspase-1 cleavage and IL-1β secretion in a dose-dependent manner.

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View All Isoforms

PDE1 PDE2 PDE3 PDE4 PDE5 PDE6 PDE7 PDE9 PDE10 PDE11 PDE12 PDE8 Autotaxin

Biological Activity
Purity & Documentation
References
Customer Review

Description

Cellular Effect

Cell Line	Type	Value	Description	References
Sf9	IC₅₀	231 nM Compound: CHEMBL430893	Inhibition of human N-terminal His6-tagged C-terminal FLAG-tagged PDE4B2 expressed in baculovirus infected sf9 cells using cAMP as substrate by microplate reader analysis Inhibition of human N-terminal His6-tagged C-terminal FLAG-tagged PDE4B2 expressed in baculovirus infected sf9 cells using cAMP as substrate by microplate reader analysis	[PMID: 26908025]

In Vitro

The increase of cAMP synthesis with the adenylate cyclase activator forskolin or the decrease of cAMP hydrolysis by the phosphodiesterase inhibitors Isobutylmethylxanthine (IBMX) and (R)-(-)-Rolipram (MedChem Express) suppresses caspase-1 cleavage and IL-1β secretion in a dose-dependent manner^[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Molecular Weight

275.34

Formula

C₁₆H₂₁NO₃

CAS No.

85416-75-7

Appearance

Solid

Color

Off-white to light yellow

SMILES

O=C1NC[C@@H](C2=CC=C(OC)C(OC3CCCC3)=C2)C1

Shipping

Room temperature in continental US; may vary elsewhere.

Storage

Powder	-20°C	3 years
	4°C	2 years
In solvent	-80°C	2 years
	-20°C	1 year

Solvent & Solubility

In Vitro:

DMSO : 100 mg/mL (363.19 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Preparing
Stock Solutions

Concentration Solvent Mass	1 mg	5 mg	10 mg

1 mM	3.6319 mL	18.1594 mL	36.3187 mL
5 mM	0.7264 mL	3.6319 mL	7.2637 mL
10 mM	0.3632 mL	1.8159 mL	3.6319 mL

View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 2 years; -20°C, 1 year. When stored at -80°C, please use it within 2 years. When stored at -20°C, please use it within 1 year.

Molarity Calculator
Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

This equation is commonly abbreviated as: C₁V₁ = C₂V₂

Concentration _(start)

Volume _(start)

Concentration _(final)

Volume _(final)

In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

Protocol 1

Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline
Solubility: ≥ 2.5 mg/mL (9.08 mM); Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.
Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
Protocol 2

Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in Saline)
Solubility: ≥ 2.5 mg/mL (9.08 mM); Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.
Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
Protocol 3

Add each solvent one by one: 10% DMSO 90% Corn Oil
Solubility: ≥ 2.5 mg/mL (9.08 mM); Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown). If the continuous dosing period exceeds half a month, please choose this protocol carefully.
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL Corn oil, and mix evenly.

In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Please enter your animal formula composition:

DMSO +

Tween-80 +

Saline

Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.

The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).

Calculation results:

Working solution concentration: mg/mL

Method for preparing stock solution: mg drug dissolved in μL DMSO (Stock solution concentration: mg/mL).

The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only. If necessary, please contact MedChemExpress (MCE).

Method for preparing in vivo working solution for animal experiments: Take μL DMSO stock solution, add μL . μL , mix evenly, next add μL Tween 80, mix evenly, then add μL Saline.

If the continuous dosing period exceeds half a month, please choose this protocol carefully.

Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.

Purity & Documentation

Purity: 99.89%

Select Batch:

Data Sheet (273 KB) SDS (252 KB)

COA (248 KB) HNMR (239 KB) LCMS (257 KB)

Handling Instructions (2659 KB)

References

[1]. Guo C, et al. Bile Acids Control Inflammation and Metabolic Disorder through Inhibition of NLRP3 Inflammasome. Immunity. 2016 Oct 18;45(4):944. [Content Brief]

Complete Stock Solution Preparation Table

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg

DMSO	1 mM	3.6319 mL	18.1594 mL	36.3187 mL	90.7968 mL
	5 mM	0.7264 mL	3.6319 mL	7.2637 mL	18.1594 mL
	10 mM	0.3632 mL	1.8159 mL	3.6319 mL	9.0797 mL
	15 mM	0.2421 mL	1.2106 mL	2.4212 mL	6.0531 mL
	20 mM	0.1816 mL	0.9080 mL	1.8159 mL	4.5398 mL
	25 mM	0.1453 mL	0.7264 mL	1.4527 mL	3.6319 mL
	30 mM	0.1211 mL	0.6053 mL	1.2106 mL	3.0266 mL
	40 mM	0.0908 mL	0.4540 mL	0.9080 mL	2.2699 mL
	50 mM	0.0726 mL	0.3632 mL	0.7264 mL	1.8159 mL
	60 mM	0.0605 mL	0.3027 mL	0.6053 mL	1.5133 mL
	80 mM	0.0454 mL	0.2270 mL	0.4540 mL	1.1350 mL
	100 mM	0.0363 mL	0.1816 mL	0.3632 mL	0.9080 mL

(R)-(-)-Rolipram Related Classifications

Neurological Disease

Help & FAQs

Do most proteins show cross-species activity?
Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

(R)-(-)-Rolipram85416-75-7(R)-Rolipram (-)-RolipramPhosphodiesterase (PDE)Inhibitorinhibitorinhibit

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(R)-(-)-Rolipram (Synonyms: (R)-Rolipram; (-)-Rolipram)

Customer Review

Other Forms of (R)-(-)-Rolipram:

(R)-(-)-Rolipram Related Antibodies

2 Publications Citing Use of MCE (R)-(-)-Rolipram

Featured Recommendations

•Related Screening Libraries:

•Related Small Molecules:

•Related Proteins:

View All Phosphodiesterase (PDE) Isoform Specific Products:

Biological Activity

Purity & Documentation

References

Customer Review

(R)-(-)-Rolipram Related Antibodies

Molarity Calculator

Dilution Calculator

Complete Stock Solution Preparation Table

(R)-(-)-Rolipram Related Classifications

Keywords:

Your Recently Viewed Products:

Customer Review

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