(±)-BI-D

Name: (±)-BI-D
Brand: MedChemExpress
SKU: HY-18601
Rating: 5.0 (6 reviews)

Cat. No.: HY-18601 Purity: 98.06%: FAQs
Data Sheet SDS COA Handling Instructions

Molarity Calculator

(±)-BI-D is a potent ALLINI(An allosteric IN inhibitor) that binds integrase at the LEDGF/p75 binding site.

For research use only. We do not sell to patients.

(±)-BI-D Chemical Structure

CAS No. : 1416258-16-6

Size	Price	Stock	Quantity
Solid + Solvent (Highly Recommended)
10 mM * 1 mL in DMSO ready for reconstitution	USD 374	In-stock	Estimated Time of Arrival: December 31
Solution
10 mM * 1 mL in DMSO	USD 374	In-stock	Estimated Time of Arrival: December 31
Solid
5 mg	USD 340	In-stock	Estimated Time of Arrival: December 31
10 mg	USD 540	In-stock	Estimated Time of Arrival: December 31
50 mg	USD 1190	In-stock	Estimated Time of Arrival: December 31
100 mg	USD 1790	In-stock	Estimated Time of Arrival: December 31
200 mg		Get quote
500 mg		Get quote

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Customer Review

Based on 6 publication(s) in Google Scholar

5 Publications Citing Use of MCE (±)-BI-D

: (±)-BI-D purchased from MedChemExpress. Usage Cited in: Cell Host Microbe. 2018 Sep 12;24(3):392-404.e8. [Abstract]; Representative MICDDRP images (scale bar, 5 µm), with quantitative assessment of 196 to 284 foci shown to the right for n=2 independent experiments, each conducted in duplicate.

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HIV HIV-1 HIV-2

Biological Activity
Purity & Documentation
References
Customer Review

Description

(±)-BI-D is a potent ALLINI(An allosteric IN inhibitor) that binds integrase at the LEDGF/p75 binding site. IC50 value: 2.4–2.9 μM(HIV-Luc infection of WT and Hdgfrp2 KO cells) [1] Target: integrase inhibitor in vitro: Approximately 2.4–2.9 μM of BI-D was required to inhibit 50% of HIV-Luc infection of WT and Hdgfrp2 KO cells, while the IC50 decreased dramatically, to 160–200 nM, in Psip1 and double-KO cells [1].

Molecular Weight

405.49

Formula

C₂₅H₂₇NO₄

CAS No.

1416258-16-6

Appearance

Solid

Color

Light yellow to yellow

SMILES

OC(C(OC(C)(C)C)C1=C(C(C=CC=C2)=C2N=C1C)C3=CC(CCCO4)=C4C=C3)=O

Shipping

Room temperature in continental US; may vary elsewhere.

Storage

Powder	-20°C	3 years
	4°C	2 years
In solvent	-80°C	2 years
	-20°C	1 year

Solvent & Solubility

In Vitro:

DMSO : ≥ 100 mg/mL (246.62 mM; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

*"≥" means soluble, but saturation unknown.

Preparing
Stock Solutions

Concentration Solvent Mass	1 mg	5 mg	10 mg

1 mM	2.4662 mL	12.3308 mL	24.6615 mL
5 mM	0.4932 mL	2.4662 mL	4.9323 mL
10 mM	0.2466 mL	1.2331 mL	2.4662 mL

View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 2 years; -20°C, 1 year. When stored at -80°C, please use it within 2 years. When stored at -20°C, please use it within 1 year.

Molarity Calculator
Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

This equation is commonly abbreviated as: C₁V₁ = C₂V₂

Concentration _(start)

Volume _(start)

Concentration _(final)

Volume _(final)

In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

Protocol 1

Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline
Solubility: ≥ 2.5 mg/mL (6.17 mM); Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.
Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
Protocol 2

Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in Saline)
Solubility: ≥ 2.5 mg/mL (6.17 mM); Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.
Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
Protocol 3

Add each solvent one by one: 10% DMSO 90% Corn Oil
Solubility: ≥ 2.5 mg/mL (6.17 mM); Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown). If the continuous dosing period exceeds half a month, please choose this protocol carefully.
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL Corn oil, and mix evenly.

In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Please enter your animal formula composition:

DMSO +

Tween-80 +

Saline

Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.

The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).

Calculation results:

Working solution concentration: mg/mL

Method for preparing stock solution: mg drug dissolved in μL DMSO (Stock solution concentration: mg/mL).

The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only. If necessary, please contact MedChemExpress (MCE).

Method for preparing in vivo working solution for animal experiments: Take μL DMSO stock solution, add μL . μL , mix evenly, next add μL Tween 80, mix evenly, then add μL Saline.

If the continuous dosing period exceeds half a month, please choose this protocol carefully.

Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.

Purity & Documentation

Purity: 98.06%

Select Batch:

Data Sheet (286 KB) SDS (251 KB)

COA (265 KB) RP-HPLC (242 KB)

Handling Instructions (2659 KB)

References

[1]. Wang H, et al. HRP2 determines the efficiency and specificity of HIV-1 integration in LEDGF/p75 knockout cells but does not contribute to the antiviral activity of a potent LEDGF/p75-binding site integrase inhibitor. Nucleic Acids Res. 2012 Dec;40(22):11518-30. [Content Brief]

[2]. Fader LD, et al. Minimizing the Contribution of Enterohepatic Recirculation to Clearance in Rat for the NCINI Class of Inhibitors of HIV. ACS Med Chem Lett. 2014 Apr 16;5(6):711-6. [Content Brief]

Complete Stock Solution Preparation Table

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg

DMSO	1 mM	2.4662 mL	12.3308 mL	24.6615 mL	61.6538 mL
	5 mM	0.4932 mL	2.4662 mL	4.9323 mL	12.3308 mL
	10 mM	0.2466 mL	1.2331 mL	2.4662 mL	6.1654 mL
	15 mM	0.1644 mL	0.8221 mL	1.6441 mL	4.1103 mL
	20 mM	0.1233 mL	0.6165 mL	1.2331 mL	3.0827 mL
	25 mM	0.0986 mL	0.4932 mL	0.9865 mL	2.4662 mL
	30 mM	0.0822 mL	0.4110 mL	0.8221 mL	2.0551 mL
	40 mM	0.0617 mL	0.3083 mL	0.6165 mL	1.5413 mL
	50 mM	0.0493 mL	0.2466 mL	0.4932 mL	1.2331 mL
	60 mM	0.0411 mL	0.2055 mL	0.4110 mL	1.0276 mL
	80 mM	0.0308 mL	0.1541 mL	0.3083 mL	0.7707 mL
	100 mM	0.0247 mL	0.1233 mL	0.2466 mL	0.6165 mL

(±)-BI-D Related Classifications

Infection

Help & FAQs

Do most proteins show cross-species activity?
Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

(±)-BI-D1416258-16-6HIVHIV IntegraseHuman immunodeficiency virusInhibitorinhibitorinhibit

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(±)-BI-D

Customer Review

(±)-BI-D Related Antibodies

5 Publications Citing Use of MCE (±)-BI-D

Featured Recommendations

•Related Screening Libraries:

•Related Small Molecules:

•Related Proteins:

View All HIV Isoform Specific Products:

Biological Activity

Purity & Documentation

References

Customer Review

Molarity Calculator

Dilution Calculator

Complete Stock Solution Preparation Table

(±)-BI-D Related Classifications

Keywords:

Your Recently Viewed Products:

Customer Review

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