A-3 hydrochloride

Name: A-3 hydrochloride
Brand: MedChemExpress
SKU: HY-125957
Rating: 4.5 (1 reviews)

Cat. No.: HY-125957 Purity: 99.75%: FAQs
Data Sheet SDS COA Handling Instructions

Molarity Calculator

A-3 hydrochloride is a potent, cell-permeable, reversible, ATP-competitive non-selective antagonist of various kinases. It against PKA (K_i=4.3 μM), casein kinase II (K_i=5.1 μM) and myosin light chain kinase (MLCK) (K_i=7.4 μM). A-3 hydrochloride also inhibits PKC and casein kinase I with K_i values of 47 μM and 80 μM, respectively.

For research use only. We do not sell to patients.

A-3 hydrochloride Chemical Structure

CAS No. : 78957-85-4

Size	Price	Stock	Quantity

Free Sample (0.1 - 0.5 mg)		Apply Now
Solid + Solvent (Highly Recommended)
10 mM * 1 mL in DMSO ready for reconstitution	USD 132	In-stock	Estimated Time of Arrival: December 31
Solution
10 mM * 1 mL in DMSO	USD 132	In-stock	Estimated Time of Arrival: December 31
Solid
5 mg	USD 77	In-stock	Estimated Time of Arrival: December 31
10 mg	USD 120	In-stock	Estimated Time of Arrival: December 31
50 mg	USD 350	In-stock	Estimated Time of Arrival: December 31
100 mg	USD 560	In-stock	Estimated Time of Arrival: December 31
200 mg		Get quote
500 mg		Get quote

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Customer Review

Based on 1 publication(s) in Google Scholar

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•Related Screening Libraries:

•Related Small Molecules:

•Related Proteins:

View All PKA Isoform Specific Products:

View All Isoforms

PKA PKAR

View All Casein Kinase Isoform Specific Products:

View All Isoforms

CK1 CK2

View All CaMK Isoform Specific Products:

View All Isoforms

CaMK III CaMK II

View All PKC Isoform Specific Products:

View All Isoforms

PKC PKCα PKCβ PKCγ PKCδ PKCε PKCη PKCζ PKCθ PKCμ PKC-ι ℩ PKCι

Biological Activity
Purity & Documentation
References
Customer Review

Description

IC₅₀ & Target^[1]

CK1

80 μM (Ki)

CK2

5.1 μM (Ki)

PKC

47 μM (Ki)

PKA

4.3 μM (Ki)

In Vitro

A-3 hydrochloride inhibits MLC-kinase competitively with respect to ATP and that the K_i value is 7.4 μM. A-3 is also a competitive inhibitor of cAMP-dependent protein kinase, cGMP-dependent protein kinase, protein kinase C, casein kinase I, and casein kinase II, with respect to ATP, exhibits K_i values of 4.3 μM, 3.8 μM, 47 μM, 80 μM, and 5.1 μM, respectively^[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Molecular Weight

321.22

Formula

C₁₂H₁₄Cl₂N₂O₂S

CAS No.

78957-85-4

Appearance

Solid

Color

Light yellow to light brown

SMILES

O=S(C1=C2C=CC=C(Cl)C2=CC=C1)(NCCN)=O.[H]Cl

Shipping

Room temperature in continental US; may vary elsewhere.

Storage

4°C, sealed storage, away from moisture

*In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)

Solvent & Solubility

In Vitro:

DMSO : 125 mg/mL (389.14 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

H₂O : 50 mg/mL (155.66 mM; Need ultrasonic)

Preparing
Stock Solutions

Concentration Solvent Mass	1 mg	5 mg	10 mg

1 mM	3.1131 mL	15.5657 mL	31.1313 mL
5 mM	0.6226 mL	3.1131 mL	6.2263 mL
10 mM	0.3113 mL	1.5566 mL	3.1131 mL

View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

Molarity Calculator
Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

This equation is commonly abbreviated as: C₁V₁ = C₂V₂

Concentration _(start)

Volume _(start)

Concentration _(final)

Volume _(final)

In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

Protocol 1

Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline
Solubility: ≥ 2.08 mg/mL (6.48 mM); Clear solution

This protocol yields a clear solution of ≥ 2.08 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (20.8 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.
Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
Protocol 2

Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in Saline)
Solubility: ≥ 2.08 mg/mL (6.48 mM); Clear solution

This protocol yields a clear solution of ≥ 2.08 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (20.8 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.
Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.

In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Calculation results:

Working solution concentration: mg/mL

This product has good water solubility, please refer to the measured solubility data in water/PBS/Saline for details.

The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only.If necessary, please contact MedChemExpress (MCE).

Purity & Documentation

Purity: 99.75%

Select Batch:

Data Sheet (274 KB) SDS (393 KB)

COA (250 KB) LCMS (92 KB)

Handling Instructions (2659 KB)

References

[1]. Inagaki M, et al. Naphthalenesulfonamides as calmodulin antagonists and protein kinase inhibitors. Mol Pharmacol. 1986 Jun;29(6):577-81. [Content Brief]

Complete Stock Solution Preparation Table

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg

H₂O / DMSO	1 mM	3.1131 mL	15.5657 mL	31.1313 mL	77.8283 mL
	5 mM	0.6226 mL	3.1131 mL	6.2263 mL	15.5657 mL
	10 mM	0.3113 mL	1.5566 mL	3.1131 mL	7.7828 mL
	15 mM	0.2075 mL	1.0377 mL	2.0754 mL	5.1886 mL
	20 mM	0.1557 mL	0.7783 mL	1.5566 mL	3.8914 mL
	25 mM	0.1245 mL	0.6226 mL	1.2453 mL	3.1131 mL
	30 mM	0.1038 mL	0.5189 mL	1.0377 mL	2.5943 mL
	40 mM	0.0778 mL	0.3891 mL	0.7783 mL	1.9457 mL
	50 mM	0.0623 mL	0.3113 mL	0.6226 mL	1.5566 mL
	60 mM	0.0519 mL	0.2594 mL	0.5189 mL	1.2971 mL
	80 mM	0.0389 mL	0.1946 mL	0.3891 mL	0.9729 mL
	100 mM	0.0311 mL	0.1557 mL	0.3113 mL	0.7783 mL

* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.