A68930 hydrochloride

Name: A68930 hydrochloride
Brand: MedChemExpress
SKU: HY-103431
Rating: 4.5 (1 reviews)

Cat. No.: HY-103431 Purity: ≥98.0%: FAQs
Data Sheet SDS COA Handling Instructions

Molarity Calculator

A68930 hydrochloride, as a dopamine D1 receptor agonist, can be used for the research of bronchiectasis.

For research use only. We do not sell to patients.

A68930 hydrochloride Chemical Structure

CAS No. : 130465-39-3

Size	Price	Stock	Quantity
1 mg	USD 110	In-stock	Estimated Time of Arrival: December 31
5 mg	USD 330	In-stock	Estimated Time of Arrival: December 31
10 mg		Get quote
50 mg		Get quote

or Bulk Inquiry

* Please select Quantity before adding items.

This product is a controlled substance and not for sale in your territory.

Customer Review

Based on 1 publication(s) in Google Scholar

Other Forms of A68930 hydrochloride:

A68930 Get quote

Featured Recommendations

•Related Screening Libraries:

•Related Small Molecules:

•Related Proteins:

View All Dopamine Receptor Isoform Specific Products:

View All Isoforms

Dopamine Receptor D₁ Receptor D₂ Receptor D₃ Receptor D₄ Receptor D₅ Receptor

Biological Activity
Purity & Documentation
References
Customer Review

Description

A68930 hydrochloride, as a dopamine D1 receptor agonist, can be used for the research of bronchiectasis^[1].

IC₅₀ & Target^[1]

D₁ Receptor

In Vitro

A68930 hydrochloride (1 μM; 5-60 minutes; 16HBE14o- or NCI-H292 cells) significantly increases phosphorylation of cAMP response element binding (CREB) protein^[1].
A68930 hydrochloride (1 μM; 48 hours; NCI-H292 cells) induces MUC5AC mRNA expression and increases the mRNA data of MUC5AC and MUC5AC protein expression^[1].
A68930 hydrochloride (1 μM; 20 minutes; NCI-H292 cells) significantly increases intracellular cAMP levels^[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Western Blot Analysis^[1]

Cell Line:	16HBE14o- or NCI-H292 cells
Concentration:	1 μM
Incubation Time:	5~60 minutes
Result:	Significantly increased phosphorylation of CREB.

RT-PCR^[1]

Cell Line:	NCI-H292 cells
Concentration:	1 μM
Incubation Time:	48 hours
Result:	Induced MUC5AC mRNA expression.

Immunofluorescence^[1]

Cell Line:	NCI-H292 cells
Concentration:	1 μM
Incubation Time:	48 hours
Result:	The mRNA data of MUC5AC, MUC5AC protein expression were increased.

Molecular Weight

307.77

Formula

C₁₆H₁₈ClNO₃

CAS No.

130465-39-3

Appearance

Solid

Color

White to yellow

SMILES

OC1=C2C[C@@H](C3=CC=CC=C3)O[C@@H](CN)C2=CC=C1O.[H]Cl

Shipping

Room temperature in continental US; may vary elsewhere.

Storage

4°C, sealed storage, away from moisture

*In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)

Solvent & Solubility

In Vitro:

DMSO : 100 mg/mL (324.92 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Preparing
Stock Solutions

Concentration Solvent Mass	1 mg	5 mg	10 mg

1 mM	3.2492 mL	16.2459 mL	32.4918 mL
5 mM	0.6498 mL	3.2492 mL	6.4984 mL
10 mM	0.3249 mL	1.6246 mL	3.2492 mL

View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Molarity Calculator
Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

This equation is commonly abbreviated as: C₁V₁ = C₂V₂

Concentration _(start)

Volume _(start)

Concentration _(final)

Volume _(final)

In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

Protocol 1

Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline
Solubility: ≥ 2.5 mg/mL (8.12 mM); Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.
Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
Protocol 2

Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in Saline)
Solubility: ≥ 2.5 mg/mL (8.12 mM); Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.
Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
Protocol 3

Add each solvent one by one: 10% DMSO 90% Corn Oil
Solubility: ≥ 2.5 mg/mL (8.12 mM); Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown). If the continuous dosing period exceeds half a month, please choose this protocol carefully.
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL Corn oil, and mix evenly.

In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Please enter your animal formula composition:

DMSO +

Tween-80 +

Saline

Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.

The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).

Calculation results:

Working solution concentration: mg/mL

Method for preparing stock solution: mg drug dissolved in μL DMSO (Stock solution concentration: mg/mL).

*In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)

The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only. If necessary, please contact MedChemExpress (MCE).

Method for preparing in vivo working solution for animal experiments: Take μL DMSO stock solution, add μL . μL , mix evenly, next add μL Tween 80, mix evenly, then add μL Saline.

If the continuous dosing period exceeds half a month, please choose this protocol carefully.

Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.

Purity & Documentation

Purity: ≥98.0%

Select Batch:

Data Sheet (270 KB) SDS (252 KB)

COA (247 KB)

Handling Instructions (2659 KB)

References

[1]. Matsuyama N, et al. The dopamine D1 receptor is expressed and induces CREB phosphorylation and MUC5AC expression in human airway epithelium. Respir Res. 2018;19(1):53. Published 2018 Apr 2. [Content Brief]

Complete Stock Solution Preparation Table

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg

DMSO	1 mM	3.2492 mL	16.2459 mL	32.4918 mL	81.2295 mL
	5 mM	0.6498 mL	3.2492 mL	6.4984 mL	16.2459 mL
	10 mM	0.3249 mL	1.6246 mL	3.2492 mL	8.1229 mL
	15 mM	0.2166 mL	1.0831 mL	2.1661 mL	5.4153 mL
	20 mM	0.1625 mL	0.8123 mL	1.6246 mL	4.0615 mL
	25 mM	0.1300 mL	0.6498 mL	1.2997 mL	3.2492 mL
	30 mM	0.1083 mL	0.5415 mL	1.0831 mL	2.7076 mL
	40 mM	0.0812 mL	0.4061 mL	0.8123 mL	2.0307 mL
	50 mM	0.0650 mL	0.3249 mL	0.6498 mL	1.6246 mL
	60 mM	0.0542 mL	0.2708 mL	0.5415 mL	1.3538 mL
	80 mM	0.0406 mL	0.2031 mL	0.4061 mL	1.0154 mL
	100 mM	0.0325 mL	0.1625 mL	0.3249 mL	0.8123 mL