2. Metabolic Enzyme/Protease
  3. Liposome
  4. AA-T3A-C12

AA-T3A-C12 is an anisamide ligand-tethered lipidoid (AA-lipidoid). AA-T3A-C12 mediates great RNA delivery and transfection of activated fibroblasts.

For research use only. We do not sell to patients.

AA-T3A-C12 Chemical Structure

AA-T3A-C12 Chemical Structure

CAS No. : 2938207-23-7

Size Price Stock Quantity
Liquid + Solvent (Highly Recommended)
10 mM * 1 mL in DMSO
ready for reconstitution
 USD 548 In-stock
Solution
10 mM * 1 mL in DMSO USD 548 In-stock
Liquid
5 mg USD 290 In-stock
10 mg USD 470 In-stock
25 mg USD 950 In-stock
50 mg USD 1500 In-stock
100 mg USD 2450 In-stock
200 mg   Get quote  
500 mg   Get quote  

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Customer Review

Based on 1 publication(s) in Google Scholar

AA-T3A-C12 Related Antibodies

Top Publications Citing Use of Products

  • Biological Activity

  • Purity & Documentation

  • References

  • Customer Review

Description

AA-T3A-C12 is an anisamide ligand-tethered lipidoid (AA-lipidoid). AA-T3A-C12 mediates great RNA delivery and transfection of activated fibroblasts[1].

In Vitro

AA-T3A-C12/siRNA LNP can enhance uptake of siRNA LNP by TGF-β-stimulated 3T3 fibroblasts[1].
AA-T3A-C12 LNP enables robust gene knockdown in activated fibroblasts[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

AA-T3A-C12 Related Antibodies
In Vivo

AA-T3A-C12/siHSP47 LNP (siRNA dose of 5 μg/mouse, i.v., twice weekly for 2 weeks) significantly down-regulates HSP47 expression in fibrotic mice[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model: CCl4-induced fibrotic mice[1]
Dosage: siRNA dose of 5 μg/mouse
Administration: i.v., twice weekly for 2 weeks
Result: led to a 65% knockdown of HSP47 (in liver) compared to PBS treatment.
Molecular Weight

1059.72

Formula

C65H126N4O6
CAS No.
Appearance

Liquid

Color

Colorless to light yellow

SMILES

OC(CCCCCCCCCC)CN(CC(O)CCCCCCCCCC)CCCN(CCCNC(C1=CC=C(OC)C=C1)=O)CCCN(CC(O)CCCCCCCCCC)CC(O)CCCCCCCCCC
Shipping

Room temperature in continental US; may vary elsewhere.
Storage
Pure form -20°C 3 years
4°C 2 years
In solvent -80°C 6 months
-20°C 1 month
Solvent & Solubility
In Vitro: 

DMSO : 100 mg/mL (94.36 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Preparing
Stock Solutions
Concentration Solvent Mass 1 mg 5 mg 10 mg
1 mM 0.9436 mL 4.7182 mL 9.4365 mL
5 mM 0.1887 mL 0.9436 mL 1.8873 mL
View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

  • Molarity Calculator

  • Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

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Volume
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Molecular Weight *

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start)

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In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

  • Protocol 1

    Add each solvent one by one:  10% DMSO    40% PEG300    5% Tween-80    45% Saline

    Solubility: 2.5 mg/mL (2.36 mM); Clear solution; Need ultrasonic

    This protocol yields a clear solution of 2.5 mg/mL.

    Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.

    Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
  • Protocol 2

    Add each solvent one by one:  10% DMSO    90% Corn Oil

    Solubility: 2.5 mg/mL (2.36 mM); Clear solution; Need ultrasonic

    This protocol yields a clear solution of 2.5 mg/mL. If the continuous dosing period exceeds half a month, please choose this protocol carefully.

    Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL Corn oil, and mix evenly.

In Vivo Dissolution Calculator
Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

g

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Please enter your animal formula composition:
%
DMSO +
% 
+
%
Tween-80 +
%
Saline
Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).
Calculation results:
Working solution concentration: mg/mL
Method for preparing stock solution: mg drug dissolved in μL  DMSO (Stock solution concentration: mg/mL).
The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only. If necessary, please contact MedChemExpress (MCE).
Method for preparing in vivo working solution for animal experiments: Take μL DMSO stock solution, add μL . μL , mix evenly, next add μL Tween 80, mix evenly, then add μL Saline.
 If the continuous dosing period exceeds half a month, please choose this protocol carefully.
Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.
Purity & Documentation

Purity: ≥98.0%

SDS (251 KB)

COA (246 KB) HNMR (141 KB) RP-HPLC (176 KB)

Handling Instructions (2659 KB)
References

Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
DMSO 1 mM 0.9436 mL 4.7182 mL 9.4365 mL 23.5911 mL
5 mM 0.1887 mL 0.9436 mL 1.8873 mL 4.7182 mL
10 mM 0.0944 mL 0.4718 mL 0.9436 mL 2.3591 mL
15 mM 0.0629 mL 0.3145 mL 0.6291 mL 1.5727 mL
20 mM 0.0472 mL 0.2359 mL 0.4718 mL 1.1796 mL
25 mM 0.0377 mL 0.1887 mL 0.3775 mL 0.9436 mL
30 mM 0.0315 mL 0.1573 mL 0.3145 mL 0.7864 mL
40 mM 0.0236 mL 0.1180 mL 0.2359 mL 0.5898 mL
50 mM 0.0189 mL 0.0944 mL 0.1887 mL 0.4718 mL
60 mM 0.0157 mL 0.0786 mL 0.1573 mL 0.3932 mL
80 mM 0.0118 mL 0.0590 mL 0.1180 mL 0.2949 mL
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AA-T3A-C12 Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

AA-T3A-C122938207-23-7LiposomeLipidoidAA-lipidoid3T3fibroblastHSP47fibrotic miceInhibitorinhibitorinhibit

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