AChE-IN-65

Cat. No.: HY-164130: FAQs
Data Sheet Handling Instructions

Molarity Calculator

AChE-IN-65 (Compound DABA_1) is a mixed inhibitor for acetylcholinesterase (AChE), with a K_i of 556.4 μM for Electrophorus electricus AChE.

For research use only. We do not sell to patients.

AChE-IN-65 Chemical Structure

CAS No. : 98704-22-4

Size	Price	Stock
5 mg	USD 108	Ask For Quote & Lead Time
10 mg	USD 162	Ask For Quote & Lead Time
25 mg	USD 324	Ask For Quote & Lead Time

* Please select Quantity before adding items.

This product is a controlled substance and not for sale in your territory.

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Biological Activity
Purity & Documentation
References
Customer Review

Description

AChE-IN-65 (Compound DABA_1) is a mixed inhibitor for acetylcholinesterase (AChE), with a K_i of 556.4 μM for Electrophorus electricus AChE.

Molecular Weight

312.23

Formula

C₁₅H₈N₂O₆

CAS No.

98704-22-4

Appearance

Solid

Color

White to off-white

SMILES

O=C(C1=CC(N2C(C=CC2=O)=O)=CC(N3C(C=CC3=O)=O)=C1)O

Shipping

Room temperature in continental US; may vary elsewhere.

Storage

-20°C, sealed storage, away from moisture and light

*In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light)

Solvent & Solubility

In Vitro:

DMSO : 100 mg/mL (320.28 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Preparing
Stock Solutions

Concentration Solvent Mass	1 mg	5 mg	10 mg

1 mM	3.2028 mL	16.0138 mL	32.0277 mL
5 mM	0.6406 mL	3.2028 mL	6.4055 mL
10 mM	0.3203 mL	1.6014 mL	3.2028 mL

View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Molarity Calculator
Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

This equation is commonly abbreviated as: C₁V₁ = C₂V₂

Concentration _(start)

Volume _(start)

Concentration _(final)

Volume _(final)

In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

Protocol 1

Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline
Solubility: ≥ 2.5 mg/mL (8.01 mM); Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.
Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
Protocol 2

Add each solvent one by one: 10% DMSO 90% Corn Oil
Solubility: ≥ 2.5 mg/mL (8.01 mM); Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown). If the continuous dosing period exceeds half a month, please choose this protocol carefully.
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL Corn oil, and mix evenly.

In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Please enter your animal formula composition:

DMSO +

Tween-80 +

Saline

Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.

The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).

Calculation results:

Working solution concentration: mg/mL

Method for preparing stock solution: mg drug dissolved in μL DMSO (Stock solution concentration: mg/mL).

*In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light)

The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only. If necessary, please contact MedChemExpress (MCE).

Method for preparing in vivo working solution for animal experiments: Take μL DMSO stock solution, add μL . μL , mix evenly, next add μL Tween 80, mix evenly, then add μL Saline.

If the continuous dosing period exceeds half a month, please choose this protocol carefully.

Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.

Purity & Documentation

Handling Instructions (2659 KB)

References

[1]. Altamirano‐Espino J A, et al. Acetylcholinesterase inhibition (potential anti‐Alzheimer effects) by aminobenzoic acid derivatives: synthesis, in vitro and in silico evaluation[J]. ChemistrySelect, 2020, 5(44): 14177-14182.

Complete Stock Solution Preparation Table

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg

DMSO	1 mM	3.2028 mL	16.0138 mL	32.0277 mL	80.0692 mL
	5 mM	0.6406 mL	3.2028 mL	6.4055 mL	16.0138 mL
	10 mM	0.3203 mL	1.6014 mL	3.2028 mL	8.0069 mL
	15 mM	0.2135 mL	1.0676 mL	2.1352 mL	5.3379 mL
	20 mM	0.1601 mL	0.8007 mL	1.6014 mL	4.0035 mL
	25 mM	0.1281 mL	0.6406 mL	1.2811 mL	3.2028 mL
	30 mM	0.1068 mL	0.5338 mL	1.0676 mL	2.6690 mL
	40 mM	0.0801 mL	0.4003 mL	0.8007 mL	2.0017 mL
	50 mM	0.0641 mL	0.3203 mL	0.6406 mL	1.6014 mL
	60 mM	0.0534 mL	0.2669 mL	0.5338 mL	1.3345 mL
	80 mM	0.0400 mL	0.2002 mL	0.4003 mL	1.0009 mL
	100 mM	0.0320 mL	0.1601 mL	0.3203 mL	0.8007 mL

AChE-IN-65 Related Classifications

Neurological Disease

Help & FAQs

Do most proteins show cross-species activity?
Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

AChE-IN-6598704-22-4Cholinesterase (ChE)mixedAlzheimer's DiseaseInhibitorinhibitorinhibit

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