2. MAPK/ERK Pathway Apoptosis
  3. JNK Apoptosis
  4. AEG3482

AEG3482 is a potent antiapoptotic compound that inhibits Jun kinase (JNK) activity through induced expression of heat shock protein 70 (HSP70). AEG3482 directly binds HSP90, thereby facilitating HSF1-dependent expression of HSP70 and HSP25.

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AEG3482 Chemical Structure

AEG3482 Chemical Structure

CAS No. : 63735-71-7

Size Price Stock Quantity
Solid + Solvent (Highly Recommended)
10 mM * 1 mL in DMSO
ready for reconstitution
 USD 110 In-stock
Solution
10 mM * 1 mL in DMSO USD 110 In-stock
Solid
5 mg USD 100 In-stock
10 mg USD 160 In-stock
25 mg USD 295 In-stock
50 mg USD 450 In-stock
100 mg USD 680 In-stock
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Customer Review

Based on 1 publication(s) in Google Scholar

AEG3482 Related Antibodies

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  • Biological Activity

  • Purity & Documentation

  • References

  • Customer Review

Description

AEG3482 is a potent antiapoptotic compound that inhibits Jun kinase (JNK) activity through induced expression of heat shock protein 70 (HSP70). AEG3482 directly binds HSP90, thereby facilitating HSF1-dependent expression of HSP70 and HSP25[1].

IC50 & Target

JNK[1]
In Vitro

AEG3482 (0.3-30 μM; 2 d) inhibits nerve growth factor (NGF) withdrawal-induced death in SCG neurons, with an EC50 of ～20 μM[1].
AEG3482 (1-80 μM; 40 h) inhibits p75NTR- and NRAGE- mediated apoptosis of PC12 cells in a dose-dependent manner[1].
AEG3482 (10-40 μM; 30 h) inhibits p75NTR- and NRAGE-mediated JNK activation in PC12 cells[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

AEG3482 Related Antibodies

Apoptosis Analysis[1]

Cell Line: PC12 cells
Concentration: 1, 2.5, 5, 10, 20, 40, 80 μM
Incubation Time: 40 hours
Result: Reduced p75NTR- or NRAGE-induced cell death by greater than 90% at the concentration of 40 μM.
Exerted a slight toxic effect in cells infected with the LacZ control at the concentration of 80 μM.

Western Blot Analysis[1]

Cell Line: PC12 cells
Concentration: 10, 20, 40 μM
Incubation Time: 30 hours
Result: Attenuated p75NTR- and NRAGE-induced c-Jun phosphorylation and caspase-3 cleavage, and the levels of c-Jun protein.
Molecular Weight

280.33

Formula

C10H8N4O2S2
CAS No.
Appearance

Solid

Color

White to light yellow

SMILES

O=S(C1=NN2C(S1)=NC(C3=CC=CC=C3)=C2)(N)=O
Shipping

Room temperature in continental US; may vary elsewhere.
Storage
Powder -20°C 3 years
4°C 2 years
In solvent -80°C 6 months
-20°C 1 month
Solvent & Solubility
In Vitro: 

DMSO : 250 mg/mL (891.81 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Preparing
Stock Solutions
Concentration Solvent Mass 1 mg 5 mg 10 mg
1 mM 3.5672 mL 17.8361 mL 35.6722 mL
5 mM 0.7134 mL 3.5672 mL 7.1344 mL
View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

  • Molarity Calculator

  • Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Mass
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Concentration
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Volume
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Molecular Weight *

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start)

C1

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Volume (start)

V1

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Concentration (final)

C2

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Volume (final)

V2

In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

  • Protocol 1

    Add each solvent one by one:  10% DMSO    40% PEG300    5% Tween-80    45% Saline

    Solubility: ≥ 2.08 mg/mL (7.42 mM); Clear solution

    This protocol yields a clear solution of ≥ 2.08 mg/mL (saturation unknown).

    Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (20.8 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.

    Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
  • Protocol 2

    Add each solvent one by one:  10% DMSO    90% Corn Oil

    Solubility: ≥ 2.08 mg/mL (7.42 mM); Clear solution

    This protocol yields a clear solution of ≥ 2.08 mg/mL (saturation unknown). If the continuous dosing period exceeds half a month, please choose this protocol carefully.

    Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (20.8 mg/mL) to 900 μL Corn oil, and mix evenly.

In Vivo Dissolution Calculator
Please enter the basic information of animal experiments:

Dosage

mg/kg

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(per animal)

g

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Please enter your animal formula composition:
%
DMSO +
% 
+
%
Tween-80 +
%
Saline
Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).
Calculation results:
Working solution concentration: mg/mL
Method for preparing stock solution: mg drug dissolved in μL  DMSO (Stock solution concentration: mg/mL).
The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only. If necessary, please contact MedChemExpress (MCE).
Method for preparing in vivo working solution for animal experiments: Take μL DMSO stock solution, add μL . μL , mix evenly, next add μL Tween 80, mix evenly, then add μL Saline.
 If the continuous dosing period exceeds half a month, please choose this protocol carefully.
Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.
Purity & Documentation
References

Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
DMSO 1 mM 3.5672 mL 17.8361 mL 35.6722 mL 89.1806 mL
5 mM 0.7134 mL 3.5672 mL 7.1344 mL 17.8361 mL
10 mM 0.3567 mL 1.7836 mL 3.5672 mL 8.9181 mL
15 mM 0.2378 mL 1.1891 mL 2.3781 mL 5.9454 mL
20 mM 0.1784 mL 0.8918 mL 1.7836 mL 4.4590 mL
25 mM 0.1427 mL 0.7134 mL 1.4269 mL 3.5672 mL
30 mM 0.1189 mL 0.5945 mL 1.1891 mL 2.9727 mL
40 mM 0.0892 mL 0.4459 mL 0.8918 mL 2.2295 mL
50 mM 0.0713 mL 0.3567 mL 0.7134 mL 1.7836 mL
60 mM 0.0595 mL 0.2973 mL 0.5945 mL 1.4863 mL
80 mM 0.0446 mL 0.2230 mL 0.4459 mL 1.1148 mL
100 mM 0.0357 mL 0.1784 mL 0.3567 mL 0.8918 mL
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AEG3482 Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

AEG348263735-71-7AEG 3482AEG-3482JNKApoptosisc-Jun N-terminal kinaseantiapoptoticJunkinaseheatshockprotein70HSP70HSP90HSF1Inhibitorinhibitorinhibit

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