Allicin (Synonyms: Diallyl thiosulfinate)

Name: Allicin
Brand: MedChemExpress
SKU: HY-N0315
Rating: 5.0 (6 reviews)

Cat. No.: HY-N0315 Purity: 97.74%: FAQs
Data Sheet SDS COA Handling Instructions

Molarity Calculator

Allicin (diallyl thiosulfinate) is isolated from garlic including Diallyl monosulfide, Diallyl disulfide, Diallyl trisulfide, Diallyl tetrasulfide, and Methyl allyl disulphide etc. They accounts for 98% of the extract. Allicin (diallyl thiosulfinate) has highly potent antimicrobial activity, and inhibits growth of a variety of microorganisms, among them antibiotic-resistant strains.

For research use only. We do not sell to patients.

Allicin Chemical Structure

CAS No. : 539-86-6

Size	Price	Stock	Quantity
Liquid + Solvent (Highly Recommended)
10 mM * 1 mL in DMSO ready for reconstitution	USD 77	In-stock	Estimated Time of Arrival: December 31
Solution
10 mM * 1 mL in DMSO	USD 77	In-stock	Estimated Time of Arrival: December 31
Liquid
50 mg	USD 70	In-stock	Estimated Time of Arrival: December 31
100 mg	USD 105	In-stock	Estimated Time of Arrival: December 31
200 mg		Get quote
500 mg		Get quote

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Based on 6 publication(s) in Google Scholar

6 Publications Citing Use of MCE Allicin

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Biological Activity
Purity & Documentation
References
Customer Review

Description

Cellular Effect

Cell Line	Type	Value	Description	References
Daudi	IC₅₀	35.3 μM Compound: Allicin	Antiproliferative activity against human Daudi cells assessed as [methyl-3H]thymidine incorporation after 16 hrs by XTT assay Antiproliferative activity against human Daudi cells assessed as [methyl-3H]thymidine incorporation after 16 hrs by XTT assay	[PMID: 18467007]
HeLa	IC₅₀	103.5 μM Compound: Allicin	Antiproliferative activity against human HeLa cells assessed as [methyl-3H]thymidine incorporation after 16 hrs by XTT assay Antiproliferative activity against human HeLa cells assessed as [methyl-3H]thymidine incorporation after 16 hrs by XTT assay	[PMID: 18467007]
HepG2	IC₅₀	39.4 μM Compound: 120	Antimalarial activity against liver stages of Plasmodium berghei assessed as human HepG2 cells assessed as inhibition of hepatic parasite growth after invasion of hepatocytes incubated for 48 hrs followed by compound wash out measured after 2 days by RT-P Antimalarial activity against liver stages of Plasmodium berghei assessed as human HepG2 cells assessed as inhibition of hepatic parasite growth after invasion of hepatocytes incubated for 48 hrs followed by compound wash out measured after 2 days by RT-P	[PMID: 22122518]
HepG2	IC₅₀	40 μM Compound: 120	Antimalarial activity against liver stages of Plasmodium yoelii 17XNL expressing GFP infected in human HepG2 cells transfected with CD81 assessed as growth inhibition of hepatic parasite after 40 hrs by flow cytometry Antimalarial activity against liver stages of Plasmodium yoelii 17XNL expressing GFP infected in human HepG2 cells transfected with CD81 assessed as growth inhibition of hepatic parasite after 40 hrs by flow cytometry	[PMID: 22122518]
HepG2	IC₅₀	6.8 μM Compound: 120	Antimalarial activity against liver stages of Plasmodium berghei infected in human HepG2 cells assessed as growth inhibition of hepatic parasite before invasion of hepatocytes incubated for 48 hrs followed by compound wash out measured after 3 days by RT- Antimalarial activity against liver stages of Plasmodium berghei infected in human HepG2 cells assessed as growth inhibition of hepatic parasite before invasion of hepatocytes incubated for 48 hrs followed by compound wash out measured after 3 days by RT-	[PMID: 22122518]
HL-60	IC₅₀	5.5 μM Compound: Allicin	Antiproliferative activity against human HL60 cells assessed as [methyl-3H]thymidine incorporation after 16 hrs by XTT assay Antiproliferative activity against human HL60 cells assessed as [methyl-3H]thymidine incorporation after 16 hrs by XTT assay	[PMID: 18467007]
HT-29	IC₅₀	83.8 μM Compound: Allicin	Antiproliferative activity against multidrug-resistant human HT-29 cells assessed as [methyl-3H]thymidine incorporation after 16 hrs by XTT assay Antiproliferative activity against multidrug-resistant human HT-29 cells assessed as [methyl-3H]thymidine incorporation after 16 hrs by XTT assay	[PMID: 18467007]
Kelly	ED₅₀	9.21 μM Compound: Allicin	Antiproliferative activity against human Kelly cells after 24 hrs Antiproliferative activity against human Kelly cells after 24 hrs	[PMID: 32786875]
NCI-N87	IC₅₀	16.8 μM Compound: Allicin	Antiproliferative activity against human NCI-N87 cells assessed as [methyl-3H]thymidine incorporation after 16 hrs by XTT assay Antiproliferative activity against human NCI-N87 cells assessed as [methyl-3H]thymidine incorporation after 16 hrs by XTT assay	[PMID: 18467007]
SGC-7901	IC₅₀	0.5 μM Compound: Allicin	Inhibition of telomerase in human SGC7901 cells by TRAP-PCR-ELISA Inhibition of telomerase in human SGC7901 cells by TRAP-PCR-ELISA	10.1039/C0MD00241K
SK-N-AS	ED₅₀	19.48 μM Compound: Allicin	Antiproliferative activity against human SK-N-AS cells after 24 hrs Antiproliferative activity against human SK-N-AS cells after 24 hrs	[PMID: 32786875]
SK-N-BE(2)-M17	ED₅₀	10.27 μM Compound: Allicin	Antiproliferative activity against human SK-N-BE(2)-M17 cells after 24 hrs Antiproliferative activity against human SK-N-BE(2)-M17 cells after 24 hrs	[PMID: 32786875]
SK-N-FI	ED₅₀	18.06 μM Compound: Allicin	Antiproliferative activity against human SK-N-FI cells after 24 hrs Antiproliferative activity against human SK-N-FI cells after 24 hrs	[PMID: 32786875]
U-937	IC₅₀	19.7 μM Compound: Allicin	Antiproliferative activity against human U937 cells assessed as [methyl-3H]thymidine incorporation after 16 hrs by XTT assay Antiproliferative activity against human U937 cells assessed as [methyl-3H]thymidine incorporation after 16 hrs by XTT assay	[PMID: 18467007]

In Vitro

Allicin exhibits comparable MICs (32-64 μg/mL) toward methicillin-resistant S. aureus ATCC 43300, the type strain S. aureus DSM 20231, E. coli DSM 30083, A. baumannii DSM 30007, and C. albicans DSM 1386^[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Clinical Trial

Molecular Weight

162.27

Formula

C₆H₁₀OS₂

CAS No.

539-86-6

Appearance

Liquid (Density: 1.112 g/cm³)

Color

Colorless to light yellow

SMILES

C=CCS(SCC=C)=O

Structure Classification

Others

Initial Source

Shipping

Room temperature in continental US; may vary elsewhere.

Storage

4°C, sealed storage, away from moisture and light

*In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light)

Solvent & Solubility

In Vitro:

DMSO : 100 mg/mL (616.26 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Ethanol : 70 mg/mL (431.38 mM; Need ultrasonic)

Preparing
Stock Solutions

Concentration Solvent Mass	1 mg	5 mg	10 mg

1 mM	6.1626 mL	30.8128 mL	61.6257 mL
5 mM	1.2325 mL	6.1626 mL	12.3251 mL
10 mM	0.6163 mL	3.0813 mL	6.1626 mL

View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Molarity Calculator
Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

This equation is commonly abbreviated as: C₁V₁ = C₂V₂

Concentration _(start)

Volume _(start)

Concentration _(final)

Volume _(final)

In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

Protocol 1

Add each solvent one by one: 10% EtOH 40% PEG300 5% Tween-80 45% Saline
Solubility: ≥ 3.5 mg/mL (21.57 mM); Clear solution

This protocol yields a clear solution of ≥ 3.5 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL EtOH stock solution (35.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.
Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
Protocol 2

Add each solvent one by one: 10% EtOH 90% (20% SBE-β-CD in Saline)
Solubility: ≥ 3.5 mg/mL (21.57 mM); Clear solution

This protocol yields a clear solution of ≥ 3.5 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL EtOH stock solution (35.0 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.
Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
Protocol 3

Add each solvent one by one: 10% EtOH 90% Corn Oil
Solubility: ≥ 3.5 mg/mL (21.57 mM); Clear solution

This protocol yields a clear solution of ≥ 3.5 mg/mL (saturation unknown). If the continuous dosing period exceeds half a month, please choose this protocol carefully.
Taking 1 mL working solution as an example, add 100 μL EtOH stock solution (35.0 mg/mL) to 900 μL Corn oil, and mix evenly.
Protocol 4

Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline
Solubility: 3.25 mg/mL (20.03 mM); Suspended solution; Need ultrasonic

This protocol yields a suspended solution of 3.25 mg/mL. Suspended solution can be used for oral and intraperitoneal injection.
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (32.5 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.
Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
Protocol 5

Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in Saline)
Solubility: ≥ 3.25 mg/mL (20.03 mM); Clear solution

This protocol yields a clear solution of ≥ 3.25 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (32.5 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.
Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
Protocol 6

Add each solvent one by one: 10% DMSO 90% Corn Oil
Solubility: ≥ 3.25 mg/mL (20.03 mM); Clear solution

This protocol yields a clear solution of ≥ 3.25 mg/mL (saturation unknown). If the continuous dosing period exceeds half a month, please choose this protocol carefully.
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (32.5 mg/mL) to 900 μL Corn oil, and mix evenly.

In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Please enter your animal formula composition:

DMSO +

Tween-80 +

Saline

Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.

The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).

Calculation results:

Working solution concentration: mg/mL

Method for preparing stock solution: mg drug dissolved in μL DMSO (Stock solution concentration: mg/mL).

*In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light)

The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only. If necessary, please contact MedChemExpress (MCE).

Method for preparing in vivo working solution for animal experiments: Take μL DMSO stock solution, add μL . μL , mix evenly, next add μL Tween 80, mix evenly, then add μL Saline.

If the continuous dosing period exceeds half a month, please choose this protocol carefully.

Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.

Purity & Documentation

Purity: ≥98.0%

Select Batch:

Data Sheet (271 KB) SDS (392 KB)

COA (304 KB) RP-HPLC (202 KB)

Handling Instructions (2659 KB)

References

[1]. Rahman, et al. Allicin and Other Functional Active Components in Garlic: Health Benefits and Bioavailability[J]. International Journal of Food Properties, 2007, 10(2):245-268.

[2]. Müller A, et al. Allicin Induces Thiol Stress in Bacteria through S-Allylmercapto Modification of ProteinCysteines. J Biol Chem. 2016 May 27;291(22):11477-90. [Content Brief]

Complete Stock Solution Preparation Table

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg

Ethanol / DMSO	1 mM	6.1626 mL	30.8128 mL	61.6257 mL	154.0642 mL
	5 mM	1.2325 mL	6.1626 mL	12.3251 mL	30.8128 mL
	10 mM	0.6163 mL	3.0813 mL	6.1626 mL	15.4064 mL
	15 mM	0.4108 mL	2.0542 mL	4.1084 mL	10.2709 mL
	20 mM	0.3081 mL	1.5406 mL	3.0813 mL	7.7032 mL
	25 mM	0.2465 mL	1.2325 mL	2.4650 mL	6.1626 mL
	30 mM	0.2054 mL	1.0271 mL	2.0542 mL	5.1355 mL
	40 mM	0.1541 mL	0.7703 mL	1.5406 mL	3.8516 mL
	50 mM	0.1233 mL	0.6163 mL	1.2325 mL	3.0813 mL
	60 mM	0.1027 mL	0.5135 mL	1.0271 mL	2.5677 mL
	80 mM	0.0770 mL	0.3852 mL	0.7703 mL	1.9258 mL
	100 mM	0.0616 mL	0.3081 mL	0.6163 mL	1.5406 mL

Allicin Related Classifications

Infection

Help & FAQs

Do most proteins show cross-species activity?
Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

Allicin539-86-6Diallyl thiosulfinateBacterialAntibioticInhibitorinhibitorinhibit

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Allicin (Synonyms: Diallyl thiosulfinate)

Customer Review

Allicin Related Antibodies

6 Publications Citing Use of MCE Allicin

Featured Recommendations

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View All Antibiotic Isoform Specific Products:

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References

Customer Review

Allicin Related Antibodies

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Complete Stock Solution Preparation Table

Allicin Related Classifications

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