2. Epigenetics PI3K/Akt/mTOR
  3. AMPK
  4. AMPK-IN-3

AMPK-IN-3 (compound 67) is a potent and selective AMPK inhibitor with IC50s of 60.7, 107 and 3820 nM for AMPK (α2), AMPK (α1) and KDR, respectively. AMPK-IN-3 inhibits AMPK does not affect cell viability or cause significant cytotoxicity in K562 cells. AMPK-IN-3 can be used in study of cancer.

For research use only. We do not sell to patients.

AMPK-IN-3 Chemical Structure

AMPK-IN-3 Chemical Structure

CAS No. : 2417674-27-0

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Based on 1 publication(s) in Google Scholar

AMPK-IN-3 Related Antibodies

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1 Publications Citing Use of MCE AMPK-IN-3

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NUAK1 NUAK2 AMPK AMPK α1β1γ1 AMPK α2β1γ1 AMPK α1β2γ1 AMPK α2β2γ1

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Description

AMPK-IN-3 (compound 67) is a potent and selective AMPK inhibitor with IC50s of 60.7, 107 and 3820 nM for AMPK (α2), AMPK (α1) and KDR, respectively. AMPK-IN-3 inhibits AMPK does not affect cell viability or cause significant cytotoxicity in K562 cells. AMPK-IN-3 can be used in study of cancer[1].

IC50 & Target[1]

AMPK (α2)

60.7 nM (IC50)

AMPK (α1)

107 nM (IC50)

KDR

3820 nM (IC50)

Cellular Effect
Cell Line Type Value Description References
K562 EC50
20.45 μM
Compound: 67
Cytotoxicity against human K562 cells assessed as reduction in cell viability incubated for 48 hrs by celltox-green assay
Cytotoxicity against human K562 cells assessed as reduction in cell viability incubated for 48 hrs by celltox-green assay
[PMID: 32334266]
In Vitro

AMPK-IN-3 (100 nM) shows inhibition values for AMPK(α2), FLT1, JAK1 JH2-pseudokinase and AMPK(α1) for 64%, 43%, 41% and 29%, respectively[1].
AMPK-IN-3 (0.195313, 0.78125, 3.125, 12.5, 50 µM; 2 h) decreases the level of p-ACC in K562 cells[1].
AMPK-IN-3 (1-100 µM; 24, 48, 72 h) shows potent inhibition of cellular AMPK activity but not affect cell viability[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

AMPK-IN-3 Related Antibodies

Cell Viability Assay[1]

Cell Line: K562 cells
Concentration: 0.195313, 0.78125, 3.125, 12.5, 50 µM
Incubation Time: 2 h
Result: Decreased cellular levels of p-ACC(Ser79) in K562 cells.

Cell Viability Assay[1]

Cell Line: K562 cells
Concentration: 1-100 µM
Incubation Time: 24, 48, 72 h
Result: Showed no measurable impact on cell viability in K562 cells cultured under hypoxic conditions for 72 hours.
Molecular Weight

451.56

Formula

C25H33N5O3
CAS No.
Appearance

Solid

Color

Yellow to brown

SMILES

O=C(/C(C1=C2)=C\C3=C(C(C(NCCN(CC)CC)=O)=C(N3)C)C)NC1=CC=C2CCC(N)=O
Shipping

Room temperature in continental US; may vary elsewhere.
Storage
Powder -20°C 3 years
4°C 2 years
In solvent -80°C 6 months
-20°C 1 month
Solvent & Solubility
In Vitro: 

DMSO : 115 mg/mL (254.67 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Preparing
Stock Solutions
Concentration Solvent Mass 1 mg 5 mg 10 mg
1 mM 2.2145 mL 11.0727 mL 22.1455 mL
5 mM 0.4429 mL 2.2145 mL 4.4291 mL
View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

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Purity & Documentation

Purity: 98.51%

SDS (251 KB)

COA (246 KB) HNMR (240 KB) LCMS (122 KB)

Handling Instructions (2659 KB)
References

Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
DMSO 1 mM 2.2145 mL 11.0727 mL 22.1455 mL 55.3636 mL
5 mM 0.4429 mL 2.2145 mL 4.4291 mL 11.0727 mL
10 mM 0.2215 mL 1.1073 mL 2.2145 mL 5.5364 mL
15 mM 0.1476 mL 0.7382 mL 1.4764 mL 3.6909 mL
20 mM 0.1107 mL 0.5536 mL 1.1073 mL 2.7682 mL
25 mM 0.0886 mL 0.4429 mL 0.8858 mL 2.2145 mL
30 mM 0.0738 mL 0.3691 mL 0.7382 mL 1.8455 mL
40 mM 0.0554 mL 0.2768 mL 0.5536 mL 1.3841 mL
50 mM 0.0443 mL 0.2215 mL 0.4429 mL 1.1073 mL
60 mM 0.0369 mL 0.1845 mL 0.3691 mL 0.9227 mL
80 mM 0.0277 mL 0.1384 mL 0.2768 mL 0.6920 mL
100 mM 0.0221 mL 0.1107 mL 0.2215 mL 0.5536 mL
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AMPK-IN-3 Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

AMPK-IN-32417674-27-0AMPKAMP-activated protein kinaseK562cancerAMPK (α2)AMPK (α1)Inhibitorinhibitorinhibit

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