Antioxidant agent-15

Name: Antioxidant agent-15
Brand: MedChemExpress
SKU: HY-20808
Rating: 4.5 (1 reviews)

Cat. No.: HY-20808 Purity: 98.97%: FAQs
Data Sheet SDS COA Handling Instructions Technical Support

Antioxidant agent-15 (Compound 4) is a potent antioxidant inhibition activity, with the IC₅₀ of 15.44 nM. Antioxidant agent-15 inhibits tumor cell growth in Hela, Hep G2 and Caco-2 cells, with the IC₅₀ of 395.26, 400.4 and 24.6 nM, respectively.

For research use only. We do not sell to patients.

Antioxidant agent-15 Chemical Structure

CAS No. : 596-38-3

Size	Stock	Quantity
100 mg	In-stock	Estimated Time of Arrival: December 31
200 mg	Get quote
500 mg	Get quote

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Customer Review

Based on 1 publication(s) in Google Scholar

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•Related Screening Libraries:

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View All COX Isoform Specific Products:

View All Isoforms

COX COX-1 COX-2 COX-3

Biological Activity
Purity & Documentation
References
Customer Review

Description

IC₅₀ & Target

95.26, 400.4 and 24.6 nM (Hela、Hep G2 and Caco-2 cells)^[1]

Cellular Effect

Cell Line	Type	Value	Description	References
DU-145	IC₅₀	> 500 μM Compound: 4a	Cytotoxicity against human DU145 cells after 48 hrs Cytotoxicity against human DU145 cells after 48 hrs	[PMID: 20129790]
HeLa	IC₅₀	> 500 μM Compound: 4a	Cytotoxicity against human HeLa cells after 48 hrs Cytotoxicity against human HeLa cells after 48 hrs	[PMID: 20129790]
MCF7	IC₅₀	> 500 μM Compound: 4a	Cytotoxicity against human MCF7 cells after 48 hrs Cytotoxicity against human MCF7 cells after 48 hrs	[PMID: 20129790]

Molecular Weight

274.31

Formula

C₁₉H₁₄O₂

CAS No.

596-38-3

Appearance

Solid

Color

White to off-white

SMILES

OC1(C2=C(OC3=C1C=CC=C3)C=CC=C2)C4=CC=CC=C4

Shipping

Room temperature in continental US; may vary elsewhere.

Storage

Powder	-20°C	3 years
	4°C	2 years
In solvent	-80°C	6 months
	-20°C	1 month

Solvent & Solubility

In Vitro:

DMSO : 100 mg/mL (364.55 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Preparing
Stock Solutions

Concentration Solvent Mass	1 mg	5 mg	10 mg

1 mM	3.6455 mL	18.2276 mL	36.4551 mL
5 mM	0.7291 mL	3.6455 mL	7.2910 mL
10 mM	0.3646 mL	1.8228 mL	3.6455 mL

View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Molarity Calculator
Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

This equation is commonly abbreviated as: C₁V₁ = C₂V₂

Concentration _(start)

Volume _(start)

Concentration _(final)

Volume _(final)

In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

Protocol 1

Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline
Solubility: ≥ 2.5 mg/mL (9.11 mM); Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.
Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
Protocol 2

Add each solvent one by one: 10% DMSO 90% Corn Oil
Solubility: ≥ 2.5 mg/mL (9.11 mM); Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown). If the continuous dosing period exceeds half a month, please choose this protocol carefully.
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL Corn oil, and mix evenly.

In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Please enter your animal formula composition:

DMSO +

Tween-80 +

Saline

Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.

The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).

Calculation results:

Working solution concentration: mg/mL

Method for preparing stock solution: mg drug dissolved in μL DMSO (Stock solution concentration: mg/mL).

The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only. If necessary, please contact MedChemExpress (MCE).

Method for preparing in vivo working solution for animal experiments: Take μL DMSO stock solution, add μL . μL , mix evenly, next add μL Tween 80, mix evenly, then add μL Saline.

If the continuous dosing period exceeds half a month, please choose this protocol carefully.

Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.

Purity & Documentation

Purity: 98.97%

Select Batch:

Data Sheet (279 KB) SDS (393 KB)

COA (259 KB) HNMR (234 KB) LCMS (87 KB)

Handling Instructions (2659 KB)

References

[1]. Abualhasan M, et al. Biological Evaluation of Xanthene and Thioxanthene Derivatives as Antioxidant, Anticancer, and COX Inhibitors. ACS Omega. 2023;8(41):38597-38606. [Content Brief]

Complete Stock Solution Preparation Table

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg

DMSO	1 mM	3.6455 mL	18.2276 mL	36.4551 mL	91.1378 mL
	5 mM	0.7291 mL	3.6455 mL	7.2910 mL	18.2276 mL
	10 mM	0.3646 mL	1.8228 mL	3.6455 mL	9.1138 mL
	15 mM	0.2430 mL	1.2152 mL	2.4303 mL	6.0759 mL
	20 mM	0.1823 mL	0.9114 mL	1.8228 mL	4.5569 mL
	25 mM	0.1458 mL	0.7291 mL	1.4582 mL	3.6455 mL
	30 mM	0.1215 mL	0.6076 mL	1.2152 mL	3.0379 mL
	40 mM	0.0911 mL	0.4557 mL	0.9114 mL	2.2784 mL
	50 mM	0.0729 mL	0.3646 mL	0.7291 mL	1.8228 mL
	60 mM	0.0608 mL	0.3038 mL	0.6076 mL	1.5190 mL
	80 mM	0.0456 mL	0.2278 mL	0.4557 mL	1.1392 mL
	100 mM	0.0365 mL	0.1823 mL	0.3646 mL	0.9114 mL