ASK1-IN-6

Cat. No.: HY-160939 Purity: 98.13%: FAQs
Data Sheet SDS COA Handling Instructions Technical Support

ASK1-IN-6 (Compound 32) is a selective inhibitor for apoptosis signal-regulating kinase 1 (ASK1), with an IC₅₀ of 25 nM. ASK1-IN-6 is blood brain barrier penetrate(rat Cl/Cl_u is 1.6/56 L/h/kg and K_p,uu is 0.46). ASK1-IN-6 exhibits anti-inflammatory avtivity and ameliorates the Alzheimer’s Disease in Tg4510 mouse model.

For research use only. We do not sell to patients.

ASK1-IN-6 Chemical Structure

CAS No. : 2407451-82-3

Size		Stock
Solid + Solvent (Highly Recommended)
10 mM * 1 mL in DMSO ready for reconstitution		Check price and availability
Solution
10 mM * 1 mL in DMSO		Check price and availability
Solid
5 mg		Check price and availability
10 mg		Check price and availability
25 mg		Check price and availability
50 mg		Check price and availability
100 mg		Check price and availability

* Please select Quantity before adding items.

This product is a controlled substance and not for sale in your territory.

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References
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Description

Molecular Weight

410.33

Formula

C₁₇H₁₄F₄N₆O₂

CAS No.

2407451-82-3

Appearance

Solid

Color

White to off-white

SMILES

FC(F)(F)[C@@H](N1C(C2=CC=CC(NC(C3=C(N=CC(F)=C3)OC)=O)=N2)=NN=C1)C

Shipping

Room temperature in continental US; may vary elsewhere.

Storage

Powder	-20°C	3 years
	4°C	2 years
In solvent	-80°C	6 months
	-20°C	1 month

Solvent & Solubility

In Vitro:

DMSO : 33.33 mg/mL (81.23 mM; ultrasonic and warming and heat to 60°C; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Preparing
Stock Solutions

Concentration Solvent Mass	1 mg	5 mg	10 mg

1 mM	2.4371 mL	12.1853 mL	24.3706 mL
5 mM	0.4874 mL	2.4371 mL	4.8741 mL
10 mM	0.2437 mL	1.2185 mL	2.4371 mL

View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Molarity Calculator
Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

This equation is commonly abbreviated as: C₁V₁ = C₂V₂

Concentration _(start)

Volume _(start)

Concentration _(final)

Volume _(final)

In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

Protocol 1

Add each solvent one by one: 10% DMSO 90% Corn Oil
Solubility: ≥ 2.5 mg/mL (6.09 mM); Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown). If the continuous dosing period exceeds half a month, please choose this protocol carefully.
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL Corn oil, and mix evenly.

In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Please enter your animal formula composition:

DMSO +

Tween-80 +

Saline

Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.

The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).

Calculation results:

Working solution concentration: mg/mL

Method for preparing stock solution: mg drug dissolved in μL DMSO (Stock solution concentration: mg/mL).

The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only. If necessary, please contact MedChemExpress (MCE).

Method for preparing in vivo working solution for animal experiments: Take μL DMSO stock solution, add μL . μL , mix evenly, next add μL Tween 80, mix evenly, then add μL Saline.

If the continuous dosing period exceeds half a month, please choose this protocol carefully.

Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.

Purity & Documentation

Purity: 98.13%

Select Batch:

Data Sheet (273 KB) SDS (251 KB)

COA (251 KB) HNMR (136 KB) RP-HPLC (248 KB) LCMS (109 KB)

Handling Instructions (2659 KB)

References

[1]. Jones JH, et al., Discovery of Potent, Selective, and Brain-Penetrant Apoptosis Signal-Regulating Kinase 1 (ASK1) Inhibitors that Modulate Brain Inflammation In Vivo. J Med Chem. 2021 Oct 28;64(20):15402-15419. [Content Brief]

Complete Stock Solution Preparation Table

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg

DMSO	1 mM	2.4371 mL	12.1853 mL	24.3706 mL	60.9266 mL
	5 mM	0.4874 mL	2.4371 mL	4.8741 mL	12.1853 mL
	10 mM	0.2437 mL	1.2185 mL	2.4371 mL	6.0927 mL
	15 mM	0.1625 mL	0.8124 mL	1.6247 mL	4.0618 mL
	20 mM	0.1219 mL	0.6093 mL	1.2185 mL	3.0463 mL
	25 mM	0.0975 mL	0.4874 mL	0.9748 mL	2.4371 mL
	30 mM	0.0812 mL	0.4062 mL	0.8124 mL	2.0309 mL
	40 mM	0.0609 mL	0.3046 mL	0.6093 mL	1.5232 mL
	50 mM	0.0487 mL	0.2437 mL	0.4874 mL	1.2185 mL
	60 mM	0.0406 mL	0.2031 mL	0.4062 mL	1.0154 mL
	80 mM	0.0305 mL	0.1523 mL	0.3046 mL	0.7616 mL

ASK1-IN-6 Related Classifications

Neurological Disease Inflammation/Immunology

Help & FAQs

Do most proteins show cross-species activity?
Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

ASK1-IN-62407451-82-3MAP3KMAP kinase kinase kinase, MEKK, MAPKKKselectiveAlzheimer's DiseaseTg4510Inhibitorinhibitorinhibit

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ASK1-IN-6

ASK1-IN-6 Related Antibodies

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